Functional Studies (Func), Antibody Production (AbP), Protein Interaction (PI), Standard (STD)
Spezifität
Optimal preservation of protein structure, post-translational modifications and functions.
Produktmerkmale
Recombinant human TGF-alpha (TGFA) (transcript variant 1) protein expressed in E.coli.
Produced with end-sequenced ORF clone
Tested for bioactivity.
Reinheit
> 98 % , as determined by Coomassie stained SDS-PAGE.
Endotoxin-Niveau
Less than 0.01 ng per μg protein as determined by the LAL method.
Biological Activity Comment
The ED50 is 0.1 - 0.4 ng/ml, corresponding to a specific activity of 0.25 - 1.0 x 10^7 units/mg, determined by the dose dependent stimulation of BALB/c-3T3 mouse embryonic fibroblast cells proliferation.
Crystallography grade
TGFA
Spezies: Human
Wirt: Insektenzellen
Recombinant
>95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
WB, SDS, ELISA, Crys
Crystallography grade
TGFA
Spezies: Human
Wirt: Insektenzellen
Recombinant
>95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
WB, SDS, ELISA, Crys
TGFA
Spezies: Human
Wirt: Escherichia coli (E. coli)
Recombinant
>95 % as determined by SDS-PAGE
Func
Active
Applikationshinweise
Recombinant human proteins can be used for: Native antigens for optimized antibody production Positive controls in ELISA and other antibody assays Protein-protein interaction In vitro biochemical assays and cell-based functional assays
Beschränkungen
Nur für Forschungszwecke einsetzbar
Konzentration
> 50 μg/mL
Buffer
1 x PBS
Lagerung
-80 °C
Informationen zur Lagerung
Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
TGF alpha is a mitogenic polypeptide that is able to bind to the EGF receptor/EGFR and to act synergistically with TGF beta to promote anchorage-independent cell proliferation in soft agar. [UniProtKB/Swiss-Prot Function]