IGFBP3 Protein (Transcript Variant 2)

Produktnummer ABIN5505673

Produktdetails

Target: IGFBP3 (Insulin-Like Growth Factor Binding Protein 3 (IGFBP3))

Protein-Typ: Recombinant

Biologische Aktivität: Active

Proteineigenschaft: Transcript Variant 2

Spezies: Human

Quelle: Escherichia coli (E. coli)

Applikation: Functional Studies (Func), Antibody Production (AbP), Protein Interaction (PI), Standard (STD)

Spezifität: Optimal preservation of protein structure, post-translational modifications and functions.

Produktmerkmale:

• Recombinant human IGFBP3 (transcript variant 2) protein expressed in E. coli.
• Produced with end-sequenced ORF clone
• Tested for bioactivity.

Reinheit: > 95 % as determined by SDS-PAGE and Coomassie blue staining

Endotoxin-Niveau: Endotoxin level is <0.1 ng/μg of protein (<1EU/μg).

Biological Activity Comment: ED50 was determined by its ability to inhibit IGF-II induced proliferation of MCF-7. The expected ED50 for this effect is less than or equal to 0.2 ug/ml in presence of 15 ng/ml of human IGF-II.

Anwendungsinformationen

Applikationshinweise: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays
Protein-protein interaction
In vitro biochemical assays and cell-based functional assays

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: Lyophilized from a 0.2 μM filtered solution of 20 mM phosphate buffer,100 mM NaCl, pH 7.2

Handhabung: Resuspend the protein in the desired concentration in proper buffer

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

Antigendetails

Target: IGFBP3 (Insulin-Like Growth Factor Binding Protein 3 (IGFBP3))

Andere Bezeichnung: IGFBP3 (IGFBP3 Produkte)

Synonyme: BP-53 Protein, IBP3 Protein, AI649005 Protein, IGFBP-3 Protein, IGgfbp3 Protein, IGF-BP3 Protein, zgc:91788 Protein, IGFBP3 Protein, igfbp3 Protein, insulin like growth factor binding protein 3 Protein, insulin-like growth factor binding protein 3 Protein, IGFBP3 Protein, Igfbp3 Protein, igfbp3 Protein, IGFBP-3 Protein, LOC100305016 Protein

Hintergrund: This gene is a member of the insulin-like growth factor binding protein (IGFBP) family and encodes a protein with an IGFBP domain and a thyroglobulin type-I domain. The protein forms a ternary complex with insulin-like growth factor acid-labile subunit (IGFALS) and either insulin-like growth factor (IGF) I or II. In this form, it circulates in the plasma, prolonging the half-life of IGFs and altering their interaction with cell surface receptors. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.

Molekulargewicht: 28.8 kDa

NCBI Accession: NP_000589

Pathways: Myometrial Relaxation and Contraction, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development, Regulation of Carbohydrate Metabolic Process, Autophagie, Smooth Muscle Cell Migration, Growth Factor Binding