Histone H3.1 Protein (His tag)

Produktnummer ABIN5505656

Produktdetails

Target: Histone H3.1 (HIST1H3B) (Histone Cluster 1, H3b (HIST1H3B))

Protein-Typ: Recombinant

Spezies: Human

Quelle: Escherichia coli (E. coli)

Aufreinigungstag / Konjugat: Dieses Histone H3.1 Protein ist gelabelt mit His tag.

Applikation: Antibody Production (AbP), Standard (STD)

Produktmerkmale:

• Recombinant human Purified recombinant protein of Human histone cluster 1, H3b (HIST1H3B), full length, with N-terminal HIS tag, expressed in E. coli, 50 μg (full length, N-term HIS tag) protein expressed in E.coli.
• Produced with end-sequenced ORF clone

Aufreinigung: Purified

Reinheit: > 80 % as determined by SDS-PAGE and Coomassie blue staining

Anwendungsinformationen

Applikationshinweise: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays

Kommentare:

The tag is located at the N-terminal.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 50 μg/mL

Buffer: 25 mM Tris, pH 8.0, 150 mM NaCl, 10 % glycerol, 1 % Sarkosyl.

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

Antigendetails

Target: Histone H3.1 (HIST1H3B) (Histone Cluster 1, H3b (HIST1H3B))

Andere Bezeichnung: histone cluster 1, H3b (HIST1H3B Produkte)

Synonyme: H3/l Protein, H3FL Protein, H3-53 Protein, histone cluster 1 H3 family member b Protein, histone cluster 1, H3b Protein, HIST1H3B Protein, Hist1h3b Protein

Hintergrund: Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3.

Molekulargewicht: 15.2 kDa

NCBI Accession: NP_003528