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IL12A & IL27B (AA 20-189) (Active) protein (His tag,AVI tag,Biotin)

Spezies: Human Wirt: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Func Active
Produktnummer ABIN4949030
  • Target
    IL12A & IL27B
    Protein-Typ
    Recombinant
    Biologische Aktivität
    Active
    Proteineigenschaft
    AA 20-189
    Spezies
    • 4
    • 3
    • 1
    • 1
    Human
    Quelle
    • 5
    • 1
    HEK-293 Cells
    Aufreinigungstag / Konjugat
    His tag,AVI tag,Biotin
    Applikation
    Functional Studies (Func)
    Marke
    MABSol®,PrecisionAvi
    Sequenz
    AA 20-189
    Spezifität
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
    Produktmerkmale
    Biotinylated Human IL23A & IL12B Heterodimer Protein, produced by co-expression of IL23A and IL12B, has a calculated MW of 21.9 kDa (IL23A) & 34.7 kDa (IL12B). Subunit IL23A is fused with a polyhistidine tag at the N-terminus and subunit IL12B contains no tag. The predicted N-terminus is His (IL23A) & Ile 23 (IL12B). The reducing (R) protein migrates as 26 kDa (IL23A) & 40-45 kDa (IL12B) respectively due to glycosylation.
    Reinheit
    >95 % as determined by SDS-PAGE.
    Endotoxin-Niveau
    Less than 1.0 EU per μg by the LAL method.
  • Kommentare

    Ready-to-use AvitagTM biotinylated protein:
    The product is exclusively produced using the AvitagTM technology. Briefly, a unique 15 amino acid peptide, the Avi tag, is introduced into the recombinant protein during expression vector construction. The single lysine residue in the Avi tag is enzymatically biotinylated by the E. Coli biotin ligase BirA.

    This single-point enzymatic labeling technique brings many advantages for commonly used binding assays. The biotinylation happens on the lysine residue of Avi tag, and therefore does NOT interfere with the target protein's natural binding activities. In addition, when immobilized on an avidin-coated surface, the protein orientation is uniform because the position of the Avi tag in the protein is precisely controlled.

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Lyophilized
    Buffer
    PBS, pH 7.4
    Handhabung
    Please avoid repeated freeze-thaw cycles.
    Lagerung
    -20 °C
  • Target
    IL12A & IL27B
    Andere Bezeichnung
    IL23A & IL12B
    Hintergrund
    Interleukin-23 subunit alpha (IL-23 alpha) can associates with IL12B to form the IL-23 interleukin, a heterodimeric cytokine which functions in innate and adaptive immunity. IL-23 may constitute with IL-17 an acute response to infection in peripheral tissues. IL-23 binds to a heterodimeric receptor complex composed of IL12RB1 and IL23R, activates the Jak-Stat signaling cascade, stimulates memory rather than naive T-cells and promotes production of proinflammatory cytokines. IL-23 induces autoimmune inflammation and thus may be responsible for autoimmune inflammatory diseases and may be important for tumorigenesis.
    Molekulargewicht
    21.9 kDa (IL23A) & 34.7 kDa (IL12B)
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