V-Set Domain Containing T Cell Activation Inhibitor 1 (VTCN1) (AA 29-258) (Active) protein (AVI tag,Biotin,His tag)

Details zu Produkt Nr. ABIN4949004, Anbieter: Anmelden zum Anzeigen
Proteinname
  • B7h4
  • B7s1
  • B7x
  • BC032925
  • B7-H4
  • B7H4
  • B7S1
  • B7X
  • B7h.5
  • PRO1291
  • VCTN1
  • RGD1311204
  • V set domain containing T cell activation inhibitor 1 L homeolog
  • V-set domain containing T cell activation inhibitor 1
  • V-set domain containing T-cell activation inhibitor 1
  • V set domain containing T cell activation inhibitor 1
  • vtcn1.L
  • VTCN1
  • vtcn1
  • Vtcn1
Proteineigenschaft
AA 29-258
7
3
3
3
2
1
1
1
1
1
1
1
1
Spezies
Human
24
7
3
2
Quelle
HEK-293 Cells
14
11
5
2
1
1
1
1
Protein-Typ
Recombinant
Biologische Aktivität
Active
Aufreinigungstag / Konjugat
Dieses VTCN1 Protein ist gelabelt mit AVI tag,Biotin,His tag.
Applikation
Functional Studies (Func)
Optionen
Hersteller
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Hersteller Produkt- Nr.
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Marke MABSol®,PrecisionAvi
Spezifität Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
Produktmerkmale This protein carries an Avi tag (Avitag™) at the C-terminus, followed by a polyhistidine tag. The protein has a calculated MW of 28 kDa. The protein migrates as 45-65 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Reinheit >90 % as determined by SDS-PAGE.
Endotoxin-Niveau Less than 1.0 EU per μg by the LAL method.
Hintergrund V-set domain-containing T-cell activation inhibitor 1 (VTCN1) is also known as Immune costimulatory protein B7-H4, Protein B7S1, T-cell costimulatory molecule B7x, B7H4, which belongs to the immunoglobulin superfamily and BTN/MOG family. VTCN1 contains two Ig-like V-type (immunoglobulin-like) domains. The expression of VTCN1 is up-regulated by IL6 and IL10 and is inhibited by GM-CSF and IL4 on antigen-presenting cells (APCs). VTCN1 / B7-H4 negatively regulates T-cell-mediated immune response by inhibiting T-cell activation, proliferation, cytokine production and development of cytotoxicity. VTCN1 involved in promoting epithelial cell transformation.
Molekulargewicht 28 kDa
NCBI Accession NP_078902
UniProt Q7Z7D3
Kommentare

Ready-to-use AvitagTM biotinylated protein:
The product is exclusively produced using the AvitagTM technology. Briefly, a unique 15 amino acid peptide, the Avi tag, is introduced into the recombinant protein during expression vector construction. The single lysine residue in the Avi tag is enzymatically biotinylated by the E. Coli biotin ligase BirA.

This single-point enzymatic labeling technique brings many advantages for commonly used binding assays. The biotinylation happens on the lysine residue of Avi tag, and therefore does NOT interfere with the target protein's natural binding activities. In addition, when immobilized on an avidin-coated surface, the protein orientation is uniform because the position of the Avi tag in the protein is precisely controlled.

Beschränkungen Nur für Forschungszwecke einsetzbar
Format Lyophilized
Rekonstitution Please see Certificate of Analysis for specific instructions. For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Buffer PBS, pH 7.4
Lagerung -20 °C
Bilder des Herstellers
SDS-PAGE (SDS) image for V-Set Domain Containing T Cell Activation Inhibitor 1 (VTCN1) (AA 29-258) (Active) protein (AVI tag,Biotin,His tag) (ABIN4949004) Biotinylated Human B7-H4 (recommended for biopanning) on SDS-PAGE under reducing (R) ...
 image for V-Set Domain Containing T Cell Activation Inhibitor 1 (VTCN1) (AA 29-258) (Active) protein (AVI tag,Biotin,His tag) (ABIN4949004) Measured by its binding ability in a functional ELISA. Immobilized Anti-B7-H4 mAb,Hum...
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