Western Blotting (WB), SDS-PAGE (SDS), ELISA, Mass Spectrometry (MS)
Verwendungszweck
Recombinant Human IDE Protein is produced by our Mammalian expression system and the target gene encoding Met42-Leu1019 is expressed with a 6His tag at the C-terminus.
Crystallography grade
IDE
Spezies: Human
Wirt: Tobacco (Nicotiana tabacum)
Recombinant
>80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
WB, SDS, ELISA
IDE
Spezies: Ratte
Wirt: Escherichia coli (E. coli)
Recombinant
> 95 %
WB, SDS, PC, Imm
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Liquid
Buffer
Supplied as a 0.2 μM filtered solution of 20 mM Tris-HCl, 150 mM NaCl, 0.05 % Brij35, 10 % Glycerol, pH 7.5.
Handhabung
Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/mL. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
Lagerung
-20 °C
Informationen zur Lagerung
Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months.