serum/glucocorticoid Regulated Kinase 1 (SGK1) (Active) Protein

Details zu Produkt Nr. ABIN411955, Anbieter: Anmelden zum Anzeigen
Proteinname
  • SGK
  • Sgk
  • cb1083
  • sgk
  • wu:fc20a09
  • serum/glucocorticoid regulated kinase 1
  • SGK1
  • Sgk1
  • sgk1
Spezies
Human
16
2
1
1
1
1
1
1
1
1
Quelle
Baculovirus infected Insect Cells
7
6
3
3
2
1
1
1
1
Protein-Typ
Recombinant
Biologische Aktivität
Active
Applikation
Functional Studies (Func), SDS-PAGE (SDS), Western Blotting (WB)
Optionen
Hersteller
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Verwendungszweck >95% Pure active Recombinant SGK1.
Produktmerkmale Protein Source: Baculovirus (Sf9 insect cells)
Reinheit SDS-PAGE: ≥95 %
Hintergrund SGK1 is a member of the serum- and glucocorticoid-induced protein kinase that is activated in vitro by 3-phosphoinositide-dependent protein kinase-1 (PDK1) and in vivo in response to signals that activate phosphatidylinositol (PI) 3-kinase (1). SGK1, mRNA is expressed in all tissues and the levels of SGK1 mRNA is increased by stimulation with serum or dexamethasone. SGK1 promotes cell survival by phosphorylating and inactivating FKHRL1. SGK and Akt display differences with respect to the efficacy with which they phosphorylate the three regulatory sites on FKHRL1. While both kinases can phosphorylate Thr-32, SGK1 displays a marked preference for Ser-315 whereas Akt favors Ser-253. These findings suggest that SGK1 and Akt may coordinately regulate the function of FKHRL1 by phosphorylating this transcription factor at distinct sites. Like PKB, SGK1 preferentially phosphorylate Ser and Thr residues that lie in Arg-Xaa-Arg-Xaa-Xaa-Ser/Thr motifs. SGK1 gene has recently been identified as an important aldosterone-induced protein kinase that mediates trafficking of the renal epithelial Na(+) channel (ENaC) to the cell membrane. Thus, SGK1 is an appealing candidate for blood pressure regulation and possibly essential hypertension (3).
Alternate Names: SGK, serum/glucocorticoid regulated kinase 1
Molekulargewicht 73.0 kDa
Gen-ID 6446
UniProt O00141
Pathways MAPK Signalweg, Notch Signalweg, Steroid Hormone Mediated Signaling Pathway
Applikationshinweise Optimal working dilution should be determined by the investigator.
Kommentare

Activity Specification: 65 nmol phosphate incorporated into Akt/SGK substrate per minute per mg protein at 30°C for 15 minutes using a final concentration of 50 μM ATP (0.83 μCi/assay).

Beschränkungen Nur für Forschungszwecke einsetzbar
Format Liquid
Konzentration 0.1 μg/μL
Buffer Recombinant proteins in storage buffer (50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, 25 % glycerol).
Konservierungsmittel Dithiothreitol (DTT)
Vorsichtsmaßnahmen This product contains dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handhabung Centrifuge the vial prior to opening.
Lagerung -80 °C
Informationen zur Lagerung -80°C
Haltbarkeit 12 months
Bilder des Herstellers
SDS-PAGE (SDS) image for serum/glucocorticoid Regulated Kinase 1 (SGK1) (Active) protein (ABIN411955) serum/glucocorticoid Regulated Kinase 1 (SGK1) (Active) protein
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