Protein Kinase C, delta (PKCd) (Active) Protein

Details zu Produkt Nr. ABIN411946, Anbieter: Anmelden zum Anzeigen
Proteinname
  • MAY1
  • PKCD
  • nPKC-delta
  • AI385711
  • D14Ertd420e
  • PKC[d]
  • PKCdelta
  • Pkcd
  • fb09d10
  • fv43b11
  • prkcd
  • wu:fb09d10
  • wu:fv43b11
  • zgc:56558
  • PKC-delta
  • may1
  • npkc-delta
  • pkc-delta2
  • pkcd
  • tpa-1
  • protein kinase C delta
  • protein kinase C, delta
  • protein kinase C, delta a
  • protein kinase C delta L homeolog
  • PRKCD
  • Prkcd
  • prkcda
  • prkcd.L
  • prkcd
Spezies
Human
15
5
2
Quelle
Baculovirus infected Insect Cells
9
4
3
2
1
1
1
Protein-Typ
Recombinant
Biologische Aktivität
Active
Applikation
Functional Studies (Func), SDS-PAGE (SDS), Western Blotting (WB)
Optionen
Hersteller
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Hersteller Produkt- Nr.
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Verwendungszweck >80% Pure active Recombinant PKC delta.
Produktmerkmale Protein Source: Baculovirus (Sf9 insect cells)
Reinheit SDS-PAGE: ≥80 %
Hintergrund Protein kinase C delta (PKC delta) is a member of the protein kinase C (PKC) family of serine-threonine kinases. It is a 79 kd protein kinase that shows strict dependence on the presence of phospholipids, but shows no activation by Ca2+ (1). Phosphatidylinositol is the most potent activator of PKC delta. Apparent kinetic constants for synthetic oligopeptides (MBP4-14, EGFR peptide and epsilon-peptide) are quite different between PKC delta and other PKCs. Northern blot analysis indicated that PKC delta is widely distributed in almost all the tissues and is a major isoform of PKC expressed in hemopoietic cells (2). PKC delta is involved in fundamental cellular functions regulated by diacylglycerols and mimicked by phorbol esters. PKC delta is partially associated with the insoluble fraction in cells even in the absence of phorbol 12-myristate 13-acetate (PMA). Upon PMA stimulation, both it translocate to the insoluble fraction of cell homogenates (3). Overexpression of PKC-delta induces significant changes in morphology and causes the cells to grow more slowly and to a decreased cell density in confluent cultures. These changes are accentuated by treatment with PMA. None of the PKC-delta overexpressers grow in soft agar with or without PMA.
Alternate Names: PKC, Protein kinase C delta
Molekulargewicht 104.0 kDa
Gen-ID 5580
NCBI Accession NM_006254
Pathways Interferon-gamma Pathway, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Thyroid Hormone Synthesis, Regulation of Actin Filament Polymerization, Carbohydrate Homeostasis, Myometrial Relaxation and Contraction, M Phase, G-protein mediated Events, Dicarboxylic Acid Transport, Positive Regulation of Response to DNA Damage Stimulus, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling
Applikationshinweise Optimal working dilution should be determined by the investigator.
Kommentare

Activity Specification: 302 nmol phosphate incorporated into CREBtide substrate per minute per mg protein at 30°C for 15 minutes using a final concentration of 50 μM ATP (0.83 μCi/assay).

Beschränkungen Nur für Forschungszwecke einsetzbar
Format Liquid
Konzentration 0.1 μg/μL
Buffer Recombinant protein in storage buffer (50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, 25 % glycerol).
Konservierungsmittel Dithiothreitol (DTT)
Vorsichtsmaßnahmen This product contains dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handhabung Centrifuge the vial prior to opening.
Lagerung -80 °C
Informationen zur Lagerung -80°C
Haltbarkeit 12 months
Bilder des Herstellers
SDS-PAGE (SDS) image for Protein Kinase C, delta (PKCd) (Active) protein (ABIN411946) Protein Kinase C, delta (PKCd) (Active) protein
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