Protein Kinase C, iota (PRKCI) (Active) Protein

Details zu Produkt Nr. ABIN411912, Anbieter: Anmelden zum Anzeigen
Proteinname
  • DXS1179E
  • PKCI
  • nPKC-iota
  • aPKC[l]
  • hal
  • has
  • PRKCI
  • PKC
  • 2310021H13Rik
  • AI427505
  • PKClambda
  • Pkci
  • Pkcl
  • Prkcl
  • aPKClambda
  • mKIAA4165
  • protein kinase C iota
  • protein kinase C, iota
  • PRKCI
  • prkci
  • Prkci
Spezies
Human
11
1
1
Quelle
Baculovirus infected Insect Cells
5
2
2
1
1
1
1
Protein-Typ
Recombinant
Biologische Aktivität
Active
Applikation
Functional Studies (Func), SDS-PAGE (SDS), Western Blotting (WB)
Optionen
Hersteller
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Hersteller Produkt- Nr.
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Verwendungszweck >90% Pure active Recombinant PKC I.
Produktmerkmale Protein Source: Baculovirus (Sf9 insect cells)
Reinheit SDS-PAGE: ≥90 %
Hintergrund PKC ι is a member of the protein kinase C family of serine-threonine kinases. The amino acid sequence of PKC ι showed greatest homology to PKC zeta, with 72 % identity overall rising to 84 % in the catalytic domain. In contrast, the homology of PKC ι to the other isoforms is less pronounced, with < 53 % identity even in the highly conserved catalytic region. PKC ι transcript is present predominantly in lung and brain, but also expressed at lower levels in many tissues including pancreatic islets. PKC ι is stimulated by tumor necrosis factor α (TNF-α) and is required for the activation of NF-kB by this cytokine. Cell transfections with a PKC ι dominant negative mutant abolished TNF-α-induced NFkB-dependent transcription. PKC ι can modify vulnerability of neural cells to apoptosis induced by amyloid β-peptide (ABP), a cytotoxic peptide linked to neuronal degeneration in Alzheimer's disease. Associated with the increased resistance to apoptosis are improved mitochondrial function and reduced activity of caspases. In addition, ABP-induced increases in levels of oxidative stress and intracellular calcium levels were attenuated in cells overexpressing PKC ι.
Alternate Names: PKC, Protein kinase C iota
Molekulargewicht ~98.0 kDa
Gen-ID 5584
NCBI Accession NM_002740
Pathways Neurotrophin Signalübertragung, Cell-Cell Junction Organization, Tube Formation
Applikationshinweise Optimal working dilution should be determined by the investigator.
Kommentare

Activity Specification: 664 nmol phosphate incorporated into CREBtide per minute per mg protein at 30°C for 15 minutes using a final concentration of 50 μM ATP (0.83 μCi/assay in 25μl reaction volume).

Beschränkungen Nur für Forschungszwecke einsetzbar
Format Liquid
Konzentration 0.1 μg/μL
Buffer Recombinant protein in storage buffer (50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, 25 % glycerol).
Konservierungsmittel Dithiothreitol (DTT)
Vorsichtsmaßnahmen This product contains dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handhabung Centrifuge the vial prior to opening.
Lagerung -80 °C
Informationen zur Lagerung -80°C
Haltbarkeit 12 months
Bilder des Herstellers
SDS-PAGE (SDS) image for Protein Kinase C, iota (PRKCI) (Active) protein (ABIN411912) Protein Kinase C, iota (PRKCI) (Active) protein
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