Neuregulin 1 (NRG1) (AA 1-525) (Active) protein (His tag)

Details zu Produkt Nr. ABIN3216389, Anbieter: Anmelden zum Anzeigen
Proteinname
  • GDF-15
  • MIC-1
  • MIC1
  • NAG-1
  • PDF
  • PLAB
  • PTGFB
  • 6030402G23Rik
  • ARIA
  • D230005F13Rik
  • GGF
  • GGFII
  • HRG
  • HRGalpha
  • Hgl
  • NDF
  • SMDF
  • GGF2
  • HGL
  • HRG1
  • HRGA
  • MST131
  • NRG-1
  • heregulin
  • nrg1-A
  • pro-neuregulin-1
  • NRG1
  • wu:fm70e01
  • DKFZp459A1838
  • AI265597
  • AW413091
  • D16jh2
  • D18020
  • Hprg
  • Hrgp
  • growth differentiation factor 15
  • neuregulin 1
  • neuregulin 1 L homeolog
  • histidine-rich glycoprotein
  • GDF15
  • Nrg1
  • NRG1
  • nrg1.L
  • nrg1
  • Hrg
Proteineigenschaft
AA 1-525
5
5
4
3
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
Spezies
Human
37
8
5
3
1
Quelle
Human Cells
28
14
6
2
2
1
Protein-Typ
Recombinant
Biologische Aktivität
Active
Aufreinigungstag / Konjugat
Dieses Neuregulin 1 Protein ist gelabelt mit His tag.
Applikation
Functional Studies (Func), SDS-PAGE (SDS)
Optionen
Hersteller
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Hersteller Produkt- Nr.
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Produktmerkmale The secreted recombinant human HPRG comprises 518 amino acids with a predicted molecular mass of 59 kDa. As a result of glycosylation, rhHPRG migrates as an approximately 75-80 kDa band in SDS-PAGE under reducing conditions.

Protein Structure: A DNA sequence encoding the human HPRG (NP_000403.1) (Met 1-Lys 525) with a C-terminal polyhistidine tag was expressed.
Predicted N-Term: Val 19
Reinheit > 97 % as determined by SDS-PAGE
Endotoxin-Niveau < 1.0 EU per μg of the protein as determined by the LAL method
Biological Activity Comment Measured by its ability to support the adhesion of MOLT-4 human acute lymphoblastic leukemia cells. (Lamb-Wharton, R.J. and W.T. Morgen, 1993, Cell Immunol. 152: 544) . Human HPRG immobilized at 1 μg/ml (100 μl/well) will induce >65% MOLT4 cell adhesion (1 x 10E5 cells/well) in the presence of 7.5 μg/ml Concanvalin A.
Hintergrund Synonyms: HPRG,HRGP,THPH11
Molekulargewicht 59 kDa, 75-80 kDa
NCBI Accession NP_000403
Pathways RTK Signalweg, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Regulation of Muscle Cell Differentiation
Applikationshinweise Optimal working dilution should be determined by the investigator.
Beschränkungen Nur für Forschungszwecke einsetzbar
Format Lyophilized
Buffer Lyophilized from sterile PBS, pH 7.4
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween80 are added as protectants before lyophilization.
Handhabung Avoid repeated freeze-thaw cycles. It is recommended that the protein be aliquoted for optimal storage.
Lagerung -20 °C,-80 °C
Informationen zur Lagerung Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Shipping: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Haltbarkeit 12 months
Bilder des Herstellers
 image for Neuregulin 1 (NRG1) (AA 1-525) (Active) protein (His tag) (ABIN3216389) Neuregulin 1 (NRG1) (AA 1-525) (Active) protein (His tag)
Allgemeine Veröffentlichungen Sørensen, Krogh-Pedersen, Petersen: "Determination of the disulphide bridge arrangement of bovine histidine-rich glycoprotein." in: FEBS letters, Vol. 328, Issue 3, pp. 285-90, 1993

Sørensen, Krogh-Pedersen, Petersen: "Determination of the disulphide bridge arrangement of bovine histidine-rich glycoprotein." in: FEBS letters, Vol. 328, Issue 3, pp. 285-90, 1993 (PubMed).

Koide, Foster, Yoshitake, Davie: "Amino acid sequence of human histidine-rich glycoprotein derived from the nucleotide sequence of its cDNA." in: Biochemistry, Vol. 25, Issue 8, pp. 2220-5, 1986 (PubMed).