FZD5 Protein (AA 27-585) (rho-1D4 tag)

Produktnummer ABIN3137184

Produktdetails

Target: FZD5 (Frizzled Family Receptor 5 (FZD5))

Protein-Typ: Recombinant

Proteineigenschaft: AA 27-585

Spezies: Maus

Quelle: Insektenzellen

Aufreinigungstag / Konjugat: Dieses FZD5 Protein ist gelabelt mit rho-1D4 tag.

Applikation: Western Blotting (WB), ELISA, SDS-PAGE (SDS), Crystallization (Crys)

Sequenz: ASKAPVCQEI TVPMCRGIGY NLTHMPNQFN HDTQDEAGLE VHQFWPLVEI HCSPDLRFFL CSMYTPICLP DYHKPLPPCR SVCERAKAGC SPLMRQYGFA WPERMSCDRL PVLGGDAEVL CMDYNRSEAT TASPKSFPAK PTLPGPPGAP SSGGECPSGG PSVCTCREPF VPILKESHPL YNKVRTGQVP NCAVPCYQPS FSPDERTFAT FWIGLWSVLC FISTSTTVAT FLIDMERFRY PERPIIFLSA CYLCVSLGFL VRLVVGHASV ACSREHSHIH YETTGPALCT VVFLLVYFFG MASSIWWVIL SLTWFLAAGM KWGNEAIAGY AQYFHLAAWL IPSVKSITAL ALSSVDGDPV AGICYVGNQN LNSLRGFVLG PLVLYLLVGT LFLLAGFVSL FRIRSVIKQG GTKTDKLEKL MIRIGIFTLL YTVPASIVVA CYLYEQHYRE SWEAALTCAC PGPDAGQPRA KPEYWVLMLK YFMCLVVGIT SGVWIWSGKT LESWRRFTSR CCCSSRRGHK SGGAMAAGDY AEASAALTGR TGPPGPTAAY HKQVSLSHV
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Produktmerkmale:

• Made in Germany - from design to production - by highly experienced protein experts.
• Mouse Fzd5 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Aufreinigung: Three step purification of membrane proteins expressed in baculovirus infected SF9 insect cells:

1. Membrane proteins are fractioned by ultracentrifugation and subsequently solubilized with different detergents (detergent screen). Samples are analyzed by Western blot.
2. The best performing detergent is used for solubilization and the proteins are purified via their rho1D4 tag via two rho1D4 antibody columns: one DTT resistant, the other one not. Eluate fractions are analyzed by Western blot.
3. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatograph. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Reinheit: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Sterilität: 0.22 μm filtered

Endotoxin-Niveau: Protein is endotoxin-free.

Güteklasse: Crystallography grade

Anwendungsinformationen

Applikationshinweise: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Kommentare:

Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C.

Haltbarkeit: Unlimited (if stored properly)

Antigendetails

Target: FZD5 (Frizzled Family Receptor 5 (FZD5))

Andere Bezeichnung: Fzd5 (FZD5 Produkte)

Synonyme: C2orf31 Protein, HFZ5 Protein, 5330434N09Rik Protein, AI427138 Protein, Fz-5 Protein, Fz5 Protein, mFz5 Protein, Xfz5 Protein, frizzled5 Protein, frizzled5a Protein, fz5 Protein, fzd5-A Protein, fz2 Protein, fz8c Protein, fzd8c Protein, zg02 Protein, frizzled class receptor 5 Protein, frizzled class receptor 5 S homeolog Protein, FZD5 Protein, Fzd5 Protein, fzd5.S Protein, fzd5 Protein

Hintergrund: Receptor for Wnt proteins. Most of frizzled receptors are coupled to the beta-catenin canonical signaling pathway, which leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes. A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. May be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues. Plays a role in yolk sac angiogenesis and in placental vascularization. Binds to Wnt2, Wnt10B, Wnt5A, but not to Wnt2B or Wnt4.

Molekulargewicht: 62.7 kDa Including tag.

UniProt: Q9EQD0

Pathways: WNT Signalweg