ABCC2 Protein (AA 1-1543) (Strep Tag)

Produktnummer ABIN3136757

Produktdetails

Target: ABCC2 (ATP-Binding Cassette, Sub-Family C (CFTR/MRP), Member 2 (ABCC2))

Protein-Typ: Recombinant

Proteineigenschaft: AA 1-1543

Spezies: Maus

Quelle: Tobacco (Nicotiana tabacum)

Aufreinigungstag / Konjugat: Dieses ABCC2 Protein ist gelabelt mit Strep Tag.

Applikation: Western Blotting (WB), SDS-PAGE (SDS), ELISA

Sequenz: MDEFCNSTFW NLSLLKSPEA DLPLCFEQTV LVWIPLGFLW LLAPWQLYRI YRSRTKRFAI TKFYLAKQVF VVCLLILAAI DLSLALTEDT GQATIPPVKY TNPILYLCTW LLVLVIQHCR QCCIQKNSWF LSMFWILSLL CGIFQFQTLI RALLQDSKSN MTYSCLFFVS YGFQIVILIL SAFSESSDST HAPSATASFL SSVTFSWYDS TVLKGYKHPL TIEDVWDIEE NLKAKSLTSK FKTIMTKDLQ KARQALQRRL KKSQQSPEGT SHGLTKKQSQ SQDVLVLEDS KKKKKKSEAT KDFPKSWLVK ALFKTFYVVI LKSFILKLAH DILLFLNPQL LKFLIGFVKD PDSYPWVGYI YAILMFSVTL IQSFFLQCYF QFCFVLGMTV RTTIIASVYK KALTLSNLAR RQYTIGETVN LMSVDSQKLM DVTNYIHLLW SSVLQIALSI FFLWRELGPS ILAGVGLMVL LVPVNGVLAT KIRKIQVQNM KNKDKRLKIM NEILSGIKIL KYFAWEPSFK EQVNSIRKKE LRNLLRFSQL QTILIFILHL TPTLVSVITF SVYVLVDSQN VLNAEKAFTS ITLFNILRFP LAMLPMVISS VIQASVSVDR LEQYLGSDDL DLSAIRHVCH FDKAVQFSEA SFTWDRDLEA TIQDVNLDIK PGQLVAVVGT VGSGKSSLIS AMLGEMENVH GHITIKGSIA YVPQQAWIQN GTIKDNILFG SEYDEKKYQR VIEACALLPD LEMLPGGDMA EIGEKGINLS GGQKHRVSLA RATYQDADIY ILDDPLSAVD THVGKHIFNK VVGPNGLLSG KTRILVTHGI HFLPQVDEIV VLGKGTILEK GSYSDLMDKK GVFAKNWKTF MKHSGPEGEA TVDNDSEEED GDCGLIPTVE EIPDDAASLT MRRENSLRRT LSRSSRSGSR RGKSLKSSLK IKSVNALNKK EEVVKGQKLI KKEFVETGKV KFSIYLKYLQ AVGWWSLLFI VIFYVLNYVA FIGTNLWLSA WTSDSEKQNG TDNSPSQRDM RIGVFGALGI AQGIFLLSSS LWSIYACRNA SKTLHRQLLT NILRAPMSFF DTTPTGRIVN RFAGDISTVD DTLPQTLRSW LLCFFGIVST LVMICMATPI FIIIIIPLSI LYVSVQVFYV ATSRQLRRLD SVTKSPIYSH FSETVSGLPV IRAFEHQQRF LANSEKQIDT NQKCVFSWIT SNRWLAIRLE LVGNLIVFCS ALLLVIYKNS LTGDTVGFVL SNALNITQTL NWLVRMTSEV ETNIVAVERI NEYINVDNEA PWVTDKKPPA DWPKKGEIQF NNYQVRYRPE LDLVLKGITC NIKSTEKVGV VGRTGAGKSS LTNCLFRILE SAGGQIIIDG IDIASIGLHD LRGRLTIIPQ DPILFSGNLR MNLDPFNKYS DEEIWRALEL AHLKSFVAGL QLGLLHEVTE GGDNLSIGQR QLLCLGRAVL RKSKILVLDE ATAAVDLETD SLIQTTIRNE FSQCTVITIA HRLHTIMDSD KIMVLDSGKI VEYGSPEELL SNMGPFYLMA KEAGIESVNH TEL
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Produktmerkmale: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Aufreinigung: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Reinheit: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin-Niveau: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Güteklasse: Crystallography grade

Anwendungsinformationen

Applikationshinweise: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Kommentare:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C.

Haltbarkeit: Unlimited (if stored properly)

Antigendetails

Target: ABCC2 (ATP-Binding Cassette, Sub-Family C (CFTR/MRP), Member 2 (ABCC2))

Andere Bezeichnung: Abcc2 (ABCC2 Produkte)

Synonyme: ABC30 Protein, CMOAT Protein, DJS Protein, MRP2 Protein, cMRP Protein, AI173996 Protein, Abc30 Protein, Cmoat Protein, Mrp2 Protein, ATP binding cassette subfamily C member 2 Protein, ATP-binding cassette, sub-family C (CFTR/MRP), member 2 Protein, ABCC2 Protein, Abcc2 Protein

Hintergrund: ATP-binding cassette sub-family C member 2 (EC 7.6.2.-) (EC 7.6.2.2) (EC 7.6.2.3) (Canalicular multispecific organic anion transporter 1) (Multidrug resistance-associated protein 2),FUNCTION: ATP-dependent transporter of the ATP-binding cassette (ABC) family that binds and hydrolyzes ATP to enable active transport of various substrates including many drugs, toxicants and endogenous compound across cell membranes. Transports a wide variety of conjugated organic anions such as sulfate-, glucuronide- and glutathione (GSH)-conjugates of endo- and xenobiotics substrates. Mediates hepatobiliary excretion of mono- and bis-glucuronidated bilirubin molecules and therefore play an important role in bilirubin detoxification. Mediates also hepatobiliary excretion of others glucuronide conjugates such as 17beta-estradiol 17-glucosiduronic acid and leukotriene C4. Transports sulfated bile salt such as taurolithocholate sulfate. Transports various anticancer drugs, such as anthracycline, vinca alkaloid and methotrexate and HIV-drugs such as protease inhibitors. {ECO:0000250|UniProtKB:Q92887}.

Molekulargewicht: 173.7 kDa

UniProt: Q8VI47

Pathways: Hormone Transport