ABCC10 Protein (AA 1-1501) (Strep Tag)

Produktnummer ABIN3136675

Produktdetails

Target: ABCC10 (ATP-Binding Cassette, Sub-Family C (CFTR/MRP), Member 10 (ABCC10))

Protein-Typ: Recombinant

Proteineigenschaft: AA 1-1501

Spezies: Maus

Quelle: Tobacco (Nicotiana tabacum)

Aufreinigungstag / Konjugat: Dieses ABCC10 Protein ist gelabelt mit Strep Tag.

Applikation: Western Blotting (WB), SDS-PAGE (SDS), ELISA

Sequenz: MEGLLAQLCG TDAARPLPLW EGDTTGHCFT QLVLSALPHA LLAVLSACHL GTPRTTNHSP ALNPGWRLRL AASFLLSIFP LLDLLPVVLP PGSRPGPLWL EVLAGCVTAV AWFTHSLALW ALVHSPHGRS RGPLALALAA FLPTPALVLT LLWHCQRGTF LPPLLPGPLG RVCLLILQLA AVLAYGLGWA APGGPQEPWT HDPFLSSESQ ETEVAEDGES WLSRFSYAWL APLLARGVRG ELQQPRDTCR LPRRLHPAFL ARVFQAHWKE GAQLWRALYR AFGCCYLALG LLKMVGTMLG FSGPLLLSLL VGFLEEGQEP LSHGLLYVLG LAGGTVISAV LQNQYGYEVR KVTLQARVAV LSTLYRKALK LGPSRPPTGE VLNLLGTDSE RLLNFAGSFH EAWGLPLQLA ITLYLLYQQV GMAFLAGLVL ALLLVPVNKV IATRIMASNQ EMLRHKDARV KLMTELLSGI RVIKFFRWEQ ALGDRVKACR TKELGRLRVI KYLDAACVYL WAALPVVICI TIFITYVLMG HQLTATKVFT ALALVRMLIL PLNNFPWVIN GLLESKVSLD RIQRFLDLPS YSPEAYYSPD PPAEPSTALE LHEALFSWDP IGASQKTFIS HLQVKKGMLV GIVGKVGCGK SSLLAAITGE LHRLCGWVAV SELSKGFGLA TQEPWIQCAT IRDNILFGKT FDAQLYREVL EACALNDDLS ILPAGDQTEV GEKGVTLSGG QRARIALARA VYQEKALYLL DDPLAAVDAD VANHLLHRCI LGVLSHTTRL LCTHRTEYLE RADVVLLMEA GQLVRTGPPS EILPLVQAVP TAWAEKEQVA TSGQSPSVCD LERTTEEELE VEQSTCGCLV QEESKSEGAV ALHVYRAYWR AMGSGLAAAI LVSLLLMQAT RNGADWWLAH WLSQLKAGRN GSREDPASCS PGSTALFSPR LLLFSPGNLY TPLLSTPLHK AASNGTADVH FYLIVYATIA GVNSLCTLLR AVLFAAGALQ AAASLHHRLL HRLLMAPVTF YDSTPSGRVL NRFSSDVACV DDSLPFLLNI LLANSVGLLG LLAVLGSGLP WLLLLLPPLS FVYYSVQGYY RASFRELRRL GSLTWSPLYS HLADTLAGLP VLRAAGATYR FEEENQRLLE LNQRCQFASY ATMQWLDIRL QLMGAAVVSA IAGIALVQHQ QGLANPGLVG LVLSYALSLT GLLSGLVSSF TQTEAMMVSV ERLEEYSCDV PQEPHSQPLQ SPHQQRISWL TQGSVEFQDV VLVYRPGLPN ALDGVTFRVE PGEKLGIVGR TGSGKSSLFL VLFRLLEPNA GRVLLDNVDT SQLELAELRS QLAVIPQEPF LFSGTIRENL DPQGLHEDRA LWQALEQCHL SEVAVAMGGL DGELGERGQN LSLGQRQLLC LARALLTDAK ILCIDEATAS VDQKTDQLLQ QTICKRFANK TVLTIAHRLN TILNSDRVLV LQAGRVVELD SPSALRNQPH SLFQQLLQSS QQGAHSGPSG C
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Produktmerkmale: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Aufreinigung: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Reinheit: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin-Niveau: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Güteklasse: Crystallography grade

Anwendungsinformationen

Applikationshinweise: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Kommentare:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C.

Haltbarkeit: Unlimited (if stored properly)

Antigendetails

Target: ABCC10 (ATP-Binding Cassette, Sub-Family C (CFTR/MRP), Member 10 (ABCC10))

Andere Bezeichnung: Abcc10 (ABCC10 Produkte)

Synonyme: EST182763 Protein, MRP7 Protein, SIMRP7 Protein, Mrp7 Protein, mFLJ00002 Protein, ATP binding cassette subfamily C member 10 Protein, ATP-binding cassette, sub-family C (CFTR/MRP), member 10 Protein, ABCC10 Protein, Abcc10 Protein

Hintergrund: ATP-binding cassette sub-family C member 10 (EC 7.6.2.2) (EC 7.6.2.3) (Multidrug resistance-associated protein 7),FUNCTION: ATP-dependent transporter of the ATP-binding cassette (ABC) family that actively extrudes physiological compounds, and xenobiotics from cells. Lipophilic anion transporter that mediates ATP-dependent transport of glucuronide conjugates such as estradiol-17-beta-o-glucuronide and GSH conjugates such as leukotriene C4 (LTC4). Does not transport glycocholic acid, taurocholic acid, MTX, folic acid, cAMP, or cGMP. May contribute to regulate the transport of organic compounds in testes across the blood-testis-barrier (By similarity). {ECO:0000250|UniProtKB:Q5T3U5}.

Molekulargewicht: 163.7 kDa

UniProt: Q8R4P9