ABCA8 Protein (AA 1-1620) (Strep Tag)

Produktnummer ABIN3136596

Produktdetails

Target: ABCA8 (ATP-Binding Cassette, Sub-Family A (ABC1), Member 8 (ABCA8))

Protein-Typ: Recombinant

Proteineigenschaft: AA 1-1620

Spezies: Maus

Quelle: Tobacco (Nicotiana tabacum)

Aufreinigungstag / Konjugat: Dieses ABCA8 Protein ist gelabelt mit Strep Tag.

Applikation: ELISA, Western Blotting (WB), SDS-PAGE (SDS)

Sequenz: MIKREISVRQ QTCALLQKNL LKKWRLKRES LMEWVSSLLL LLFLYWYPHG HGATDLSSVP TKDLGRVDSF RQSGFMIGYT PVTSMTQQIM EKVAATPFMA DKKVLGLLDE ENIKELTESH AEIIRVIFSD TFSYHLKFQF DQRIPTSREL RDHNAHCDGL YEDVNCLIAI FWKEGFVALQ AAINAAIIET TTNHSVMEEL LSVSGKFMKI HPFVRQEGIL TDFFIFTCII SFSPITYYVS INVARERKRM KGLMMMMGLR DPAFWLSWGL LYAGFVFIMA LSLALVIKSV QFFILTSFMV VFSLFLLYGL SMITLAFLMS ALVRKSVLTG LSVFLLTIFW GSLGFTSLYR YLPAPVEWTL SLFSPFAFTL GMAQLLRVDY DLNSNAPPDP ASGSNLIIAT NFMLVFDAFL YLALMMYFEK VLPNEYGHQH SPLFFLKSSF WLQTRKPAHV ILEDGIDPVP SSGDSFEPVS PEFHGKESIR IRNISKEYKG KPNKIEALKD LTLDIYEGQI TAVLGHSGAG KSTLLNILSG LSVPTKGSVT IYNNNLSEMA DLENILRIAG VCPQANVQFD FLTVRENLRL FAKIRGIPPQ DVEKEVQRVL LELEMKNIQN ILAQNLSGGQ KRKLTFGIAI LGDSQIFLLD EPTAGLDPFS RHRVWNLLKE RRADRVVLFS TQFMDEADIL ADRKVFISNG RLKCAGSSLF LKKKWGVGYH LSLQLKEVCV PENITSLVKQ HIPAAKLSAE GEGKLLYTLP LETTYRFPEL CQSLDSCPGL GIENYGVSMT TLNEVFLKLE GKASIDEPEV DIVGEGQTER SGDTERLMEM EQTLSSLRET EKTDGMALWR QQTCAIAKVR LLKLKHERKT LLSVLLILVV GICPFLFENI STKIRQSSYT WELSPHDYFL APGQQPQGML TQLLIINKTE ASIDDFIHSV ERQNIALEVD ASGTRDGTDD PSYNGALIVS GNEKNHSFSF ACNTKRLNCF PVLMDILSNG LLGMVKPSAR IQTDRSTYLM DETIHPLEDL WKTAFWLILT SACPPYIAMS SVTDYKNRAW FQLRVSGLFP SAYWVGQAMV DIPLYCFVFL FMSLMDYLFR FPDTMFSIIS HVIQIPCSVG YAISLIFLTY VISFISRKGK KNSGIWSLSF YIITVFSVAV ILLAFDVDGT QYYIIFLIPP STLVGCLILS LHLFIGQIFE EGQVIEPFLV FLIPFLHVFI FIFTLRCLEW KFGKKTMRKD PIFRISPRNN DVYQNPEEPE DEDEDVQMER MRTANALVST SFDEKPVIIA SCLRKEYAGK QKHCLSKKKA KIATRNVSFC VRKGEILGLL GHNGAGKSTS LKMISGDTKV TAGQVLLKGS REGDTPGFLG YCPQENALWP NLTVKEHLEI FAAVRGLRKS HAAVAITRLA DALKLQDQLK SPVKTLSEGV KRKLCFVLSI LGNPSILLLD EPSTGLDPEG QQQIWQAIRA IIKNTDRGAL LTTHYMAEAE ALCDRVAILV SGRLRCIGSI QHLKSKFGKD YLLEMKVKTL EQVEPLNTEI LRLFPQASRQ ERYSSLMAYK LPVEAVQPLS QAFFKLEKVK QTFDLEEYSL SQSTLEQVFL ELSKEQELDG LELEELDSSI KWKLLPQEEA
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Produktmerkmale: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Aufreinigung: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Reinheit: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin-Niveau: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Güteklasse: Crystallography grade

Anwendungsinformationen

Applikationshinweise: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Kommentare:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C.

Haltbarkeit: Unlimited (if stored properly)

Antigendetails

Target: ABCA8 (ATP-Binding Cassette, Sub-Family A (ABC1), Member 8 (ABCA8))

Andere Bezeichnung: Abca8b (ABCA8 Produkte)

Synonyme: Abca8b Protein, ABCA8 Protein, abca8a Protein, Abca8 Protein, ATP binding cassette subfamily A member 8 Protein, ATP binding cassette subfamily A member 9 Protein, ATP-binding cassette sub-family A member 8 Protein, ATP-binding cassette, sub-family A (ABC1), member 8b Protein, ABCA8 Protein, ABCA9 Protein, Abca8 Protein, abca8 Protein, LOC100473721 Protein, LOC100520512 Protein, Abca8b Protein

Hintergrund: ABC-type organic anion transporter ABCA8B (EC 7.6.2.-) (ATP-binding cassette sub-family A member 8B),FUNCTION: Mediates cholesterol and taurocholate efflux (PubMed:28882873). Through the interaction with ABCA1 potentiates the cholesterol efflux to lipid-free APOA1, in turn regulates high-density lipoprotein cholesterol levels (By similarity). {ECO:0000250|UniProtKB:O94911, ECO:0000269|PubMed:28882873}.

Molekulargewicht: 183.0 kDa

UniProt: Q8K440