KCNMB1 Protein (AA 2-191) (rho-1D4 tag)

Produktnummer ABIN3125809

Produktdetails

Target: KCNMB1 (Potassium Large Conductance Calcium-Activated Channel, Subfamily M, beta Member 1 (KCNMB1))

Protein-Typ: Recombinant

Proteineigenschaft: AA 2-191

Spezies: Maus

Quelle: Insektenzellen

Aufreinigungstag / Konjugat: Dieses KCNMB1 Protein ist gelabelt mit rho-1D4 tag.

Applikation: ELISA, Western Blotting (WB), Crystallization (Crys), SDS-PAGE (SDS)

Sequenz: GKKLVMAQKR GETRALCLGV AMVVCAAITY YVLGTTVLPL YQKSVWTQES ICHLIETNIK DQEELEGKKV PQYPCLWVNV SAVGRWAMLY HTEDTRDQNQ QCSYIPRNLD NYQTALADVK KVRANFYKHH EFYCLSAPQV NETSVVYQRL YGPQVLLFSF FWPTFLLTGG LLLIAMVKLN RSLSILAAQK
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Produktmerkmale:

• Made in Germany - from design to production - by highly experienced protein experts.
• Mouse Kcnmb1 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Aufreinigung: Three step purification of membrane proteins expressed in baculovirus infected SF9 insect cells:

1. Membrane proteins are fractioned by ultracentrifugation and subsequently solubilized with different detergents (detergent screen). Samples are analyzed by Western blot.
2. The best performing detergent is used for solubilization and the proteins are purified via their rho1D4 tag via two rho1D4 antibody columns: one DTT resistant, the other one not. Eluate fractions are analyzed by Western blot.
3. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatograph. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Reinheit: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Sterilität: 0.22 μm filtered

Endotoxin-Niveau: Protein is endotoxin-free.

Güteklasse: Crystallography grade

Anwendungsinformationen

Applikationshinweise: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Kommentare:

Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C.

Haltbarkeit: Unlimited (if stored properly)

Antigendetails

Target: KCNMB1 (Potassium Large Conductance Calcium-Activated Channel, Subfamily M, beta Member 1 (KCNMB1))

Andere Bezeichnung: Kcnmb1 (KCNMB1 Produkte)

Synonyme: KCNMB1 Protein, BKbeta1 Protein, K(VCA)beta Protein, SLO-BETA Protein, hbeta1 Protein, hslo-beta Protein, k(VCA)beta-1 Protein, slo-beta-1 Protein, BKBETA Protein, potassium calcium-activated channel subfamily M regulatory beta subunit 1 Protein, potassium large conductance calcium-activated channel, subfamily M, beta member 1 Protein, KCNMB1 Protein, Kcnmb1 Protein

Hintergrund: Regulatory subunit of the calcium activated potassium KCNMA1 (maxiK) channel. Modulates the calcium sensitivity and gating kinetics of KCNMA1, thereby contributing to KCNMA1 channel diversity. Increases the apparent Ca(2+)/voltage sensitivity of the KCNMA1 channel. It also modifies KCNMA1 channel kinetics and alters its pharmacological properties. It slows down the activation and the deactivation kinetics of the channel. Acts as a negative regulator of smooth muscle contraction by enhancing the calcium sensitivity to KCNMA1. Its presence is also a requirement for internal binding of the KCNMA1 channel opener dehydrosoyasaponin I (DHS-1) triterpene glycoside and for external binding of the agonist hormone 17-beta-estradiol (E2). Increases the binding activity of charybdotoxin (CTX) toxin to KCNMA1 peptide blocker by increasing the CTX association rate and decreasing the dissociation rate. {ECO:0000269|PubMed:11057658}.

Molekulargewicht: 22.9 kDa Including tag.

UniProt: Q8CAE3