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MGAT4A Protein (AA 28-535) (His tag)

Crystallography grade MGAT4A Spezies: Maus Wirt: Escherichia coli (E. coli) Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, Crys, SDS
Produktnummer ABIN3153340
  • Target Alle MGAT4A Proteine anzeigen
    MGAT4A (Mannosyl (Alpha-1,3-)-Glycoprotein beta-1,4-N-Acetylglucosaminyltransferase, Isozyme A (MGAT4A))
    Protein-Typ
    Recombinant
    Proteineigenschaft
    AA 28-535
    Spezies
    • 4
    • 3
    • 1
    Maus
    Quelle
    • 4
    • 2
    • 1
    • 1
    Escherichia coli (E. coli)
    Aufreinigungstag / Konjugat
    Dieses MGAT4A Protein ist gelabelt mit His tag.
    Applikation
    ELISA, Western Blotting (WB), Crystallization (Crys), SDS-PAGE (SDS)
    Sequenz
    QNGKEKLIAY QREFLALKER LRVAEHRISQ RSSELNTIVQ QFRRAGAETN GSKTALSTIS DNTIKLLKEL TSKKSLRVPS IYYHLPHLLQ NERSLQPAVQ IGSGRTGVSI VMGIPTVKRE VKSYLVETLH SLIDNLYPEE KLDCVIVVFI GETDLDYVHS VVANLEKEFS REISSGLLEI ISPPESYYPD LTNLKETFGD SKERVRWRTK QNLDYCFLMM YAQEKGIYYI QLEDDIIVKQ NYFNTIKNFA LQLSSEEWMI LEFSQLGFIG KMFQAPDLAL VVEFILMFYK EKPIDWLLDH ILWVKVCNPE KDAKHCDRQK ANLRIRFRPS LFQHVGLHSS LSGKIQKLTD KDYMKPLLLK VHVNPPAEVS TSLKVYQGHT LEKTYMGEDF FWAITPTAGD YILFKFDKPV NVESYLFHSG NQEHPGDILL NTTVDVLPLK SDSLEISKET KDKRLEDGYF RIGKFEYGVA EGIVDPGLNP ISAFRLSVIQ NSAVWAILNE IHIKKVTS
    Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
    Produktmerkmale
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Mouse Mgat4a Protein (raised in E. Coli) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    Aufreinigung
    Two step purification of proteins expressed in bacterial culture:
    1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
    2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    Reinheit
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    Sterilität
    0.22 μm filtered
    Endotoxin-Niveau
    Endotoxin has not been removed. Please contact us if you require endotoxin removal.
    Güteklasse
    Crystallography grade
    Top Product
    Discover our top product MGAT4A Protein
  • Applikationshinweise
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
    Kommentare

    Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Buffer
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    Handhabung
    Avoid repeated freeze-thaw cycles.
    Lagerung
    -80 °C
    Informationen zur Lagerung
    Store at -80°C.
    Haltbarkeit
    Unlimited (if stored properly)
  • Target
    MGAT4A (Mannosyl (Alpha-1,3-)-Glycoprotein beta-1,4-N-Acetylglucosaminyltransferase, Isozyme A (MGAT4A))
    Andere Bezeichnung
    Mgat4a (MGAT4A Produkte)
    Synonyme
    GNT-IV Protein, GNT-IVA Protein, GnT-4a Protein, 9530018I07Rik Protein, GnT-IVa Protein, glcNAc-T-IVa Protein, mannosyl (alpha-1,3-)-glycoprotein beta-1,4-N-acetylglucosaminyltransferase, isozyme A Protein, mannoside acetylglucosaminyltransferase 4, isoenzyme A Protein, MGAT4A Protein, Mgat4a Protein
    Hintergrund
    Glycosyltransferase that participates in the transfer of N-acetylglucosamine (GlcNAc) to the core mannose residues of N-linked glycans. Catalyzes the formation of the GlcNAcbeta1-4 branch on the GlcNAcbeta1-2Manalpha1-3 arm of the core structure of N-linked glycans. Essential for the production of tri- and tetra-antennary N-linked sugar chains. Involved in glucose transport by mediating SLC2A2/GLUT2 glycosylation, thereby controlling cell-surface expression of SLC2A2 in pancreatic beta cells. {ECO:0000269|PubMed:16377570}.
    Molekulargewicht
    59.3 kDa Including tag.
    UniProt
    Q812G0
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