IGF1R Protein (AA 742-1373) (rho-1D4 tag)

Produktnummer ABIN3135297

Produktdetails

Target: IGF1R (Insulin-Like Growth Factor 1 Receptor (IGF1R))

Protein-Typ: Recombinant

Proteineigenschaft: AA 742-1373

Spezies: Maus

Quelle: Insektenzellen

Aufreinigungstag / Konjugat: Dieses IGF1R Protein ist gelabelt mit rho-1D4 tag.

Applikation: SDS-PAGE (SDS), Western Blotting (WB), ELISA, Crystallization (Crys)

Sequenz: DVMQVANTTM SSRSRNTTVA DTYNITDPEE FETEYPFFES RVDNKERTVI SNLRPFTLYR IDIHSCNHEA EKLGCSASNF VFARTMPAEG ADDIPGPVTW EPRPENSIFL KWPEPENPNG LILMYEIKYG SQVEDQRECV SRQEYRKYGG AKLNRLNPGN YTARIQATSL SGNGSWTDPV FFYVPAKTTY ENFMHLIIAL PVAILLIVGG LVIMLYVFHR KRNNSRLGNG VLYASVNPEY FSAADVYVPD EWEVAREKIT MNRELGQGSF GMVYEGVAKG VVKDEPETRV AIKTVNEAAS MRERIEFLNE ASVMKEFNCH HVVRLLGVVS QGQPTLVIME LMTRGDLKSY LRSLRPEVEQ NNLVLIPPSL SKMIQMAGEI ADGMAYLNAN KFVHRDLAAR NCMVAEDFTV KIGDFGMTRD IYETDYYRKG GKGLLPVRWM SPESLKDGVF TTHSDVWSFG VVLWEIATLA EQPYQGLSNE QVLRFVMEGG LLDKPDNCPD MLFELMRMCW QYNPKMRPSF LEIIGSIKDE MEPSFQEVSF YYSEENKPPE PEELEMELEM EPENMESVPL DPSASSASLP LPERHSGHKA ENGPGPGVLV LRASFDERQP YAHMNGGRAN ERALPLPQSS T
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Produktmerkmale:

• Made in Germany - from design to production - by highly experienced protein experts.
• Mouse Igf1r Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Aufreinigung: Three step purification of membrane proteins expressed in baculovirus infected SF9 insect cells:

1. Membrane proteins are fractioned by ultracentrifugation and subsequently solubilized with different detergents (detergent screen). Samples are analyzed by Western blot.
2. The best performing detergent is used for solubilization and the proteins are purified via their rho1D4 tag via two rho1D4 antibody columns: one DTT resistant, the other one not. Eluate fractions are analyzed by Western blot.
3. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatograph. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Reinheit: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Sterilität: 0.22 μm filtered

Endotoxin-Niveau: Protein is endotoxin-free.

Güteklasse: Crystallography grade

Anwendungsinformationen

Applikationshinweise: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Kommentare:

Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C.

Haltbarkeit: Unlimited (if stored properly)

Antigendetails

Target: IGF1R (Insulin-Like Growth Factor 1 Receptor (IGF1R))

Andere Bezeichnung: Igf1r (IGF1R Produkte)

Synonyme: CMT2N Protein, CD221 Protein, IGFIR Protein, IGFR Protein, JTK13 Protein, IGFIRC Protein, IGFR1 Protein, IGF-1R Protein, IGF1R Protein, DKFZp469I0618 Protein, igf-1r Protein, xIGF-1R Protein, xIGFR Protein, xigf1r Protein, A330103N21Rik Protein, D930020L01 Protein, hyft Protein, igf-ir Protein, IGF-IR Protein, IGF-IRb Protein, wu:fb03h06 Protein, IGF-IRa Protein, sb:eu1090 Protein, wu:fi20b10 Protein, alanyl-tRNA synthetase Protein, insulin like growth factor 1 receptor Protein, insulin-like growth factor 1 receptor Protein, insulin-like growth factor 1 Protein, insulin-like growth factor I receptor Protein, insulin like growth factor 1 receptor S homeolog Protein, insulin-like growth factor 1b receptor Protein, insulin-like growth factor 1a receptor Protein, AARS Protein, IGF1R Protein, Igf1r Protein, igf1r Protein, Igf1 Protein, LOC780503 Protein, LOC100136427 Protein, igf1r.S Protein, igf1rb Protein, igf1ra Protein

Hintergrund: Receptor tyrosine kinase which mediates actions of insulin-like growth factor 1 (IGF1). Binds IGF1 with high affinity and IGF2 and insulin (INS) with a lower affinity. The activated IGF1R is involved in cell growth and survival control. IGF1R is crucial for tumor transformation and survival of malignant cell. Ligand binding activates the receptor kinase, leading to receptor autophosphorylation, and tyrosines phosphorylation of multiple substrates, that function as signaling adapter proteins including, the insulin-receptor substrates (IRS1/2), Shc and 14-3-3 proteins. Phosphorylation of IRSs proteins lead to the activation of two main signaling pathways: the PI3K-AKT/PKB pathway and the Ras-MAPK pathway. The result of activating the MAPK pathway is increased cellular proliferation, whereas activating the PI3K pathway inhibits apoptosis and stimulates protein synthesis. Phosphorylated IRS1 can activate the 85 kDa regulatory subunit of PI3K (PIK3R1), leading to activation of several downstream substrates, including protein AKT/PKB. AKT phosphorylation, in turn, enhances protein synthesis through mTOR activation and triggers the antiapoptotic effects of IGFIR through phosphorylation and inactivation of BAD. In parallel to PI3K-driven signaling, recruitment of Grb2/SOS by phosphorylated IRS1 or Shc leads to recruitment of Ras and activation of the ras-MAPK pathway. In addition to these two main signaling pathways IGF1R signals also through the Janus kinase/signal transducer and activator of transcription pathway (JAK/STAT). Phosphorylation of JAK proteins can lead to phosphorylation/activation of signal transducers and activators of transcription (STAT) proteins. In particular activation of STAT3, may be essential for the transforming activity of IGF1R. The JAK/STAT pathway activates gene transcription and may be responsible for the transforming activity. JNK kinases can also be activated by the IGF1R. IGF1 exerts inhibiting activities on JNK activation via phosphorylation and inhibition of MAP3K5/ASK1, which is able to directly associate with the IGF1R (By similarity). When present in a hybrid receptor with INSR, binds IGF1 (By similarity). {ECO:0000250}.

Molekulargewicht: 72.6 kD. Including tag.

UniProt: Q60751

Pathways: RTK Signalweg, Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Autophagie