ATP13A3 Protein (AA 1-1219) (Strep Tag)

Produktnummer ABIN3135252

Produktdetails

Target: ATP13A3 (ATPase Type 13A3 (ATP13A3))

Protein-Typ: Recombinant

Proteineigenschaft: AA 1-1219

Spezies: Maus

Quelle: Tobacco (Nicotiana tabacum)

Aufreinigungstag / Konjugat: Dieses ATP13A3 Protein ist gelabelt mit Strep Tag.

Applikation: SDS-PAGE (SDS), Western Blotting (WB), ELISA

Sequenz: MDKEERKTIN KGQEDEMEIH GYNLCRWKLA MVFVGVICTG GFLLLLLYWL PEWRVKATCV RAAVKDCEVV LLRTTDEFRV WFCAKIHFLP VENQPNLNAK CLVNEVSNGH AVHLTEENRC EMNKYSQSQS QQMRYFTHHS IRYFWNDAIH NFDFLKGLDE GVSCASLYEK HSAGLTQGMH AYRKLIYGVN EIAVKVPSVF KLLIKEVLNP FYIFQLFSVI LWSVDEYYYY ALAIVIMSVV SIISSLYSIR KQYVMLHDMV ATHSTVRVSV CRENEEIEEI FSTDLVPGDV MIIPLNGTVM PCDAVLINGT CIVNESMLTG ESVPVTKTNL PNPSVDVKGM GEEQYSPETH KRHTLFCGTT VIQTRFYTGE LVKAIVVRTG FSTSKGQLVR SILYPKPTDF KLYRDAYLFL LCLVVVAGIG FIYTIINSIL NEKEVQEIII KSLDIITITV PPALPAAMTA GIVYAQRRLK KVGIFCISPQ RINICGQLNL VCFDKTGTLT EDGLDLWGIQ RVENTRFLLP EDNVCSEMLV KSQFVACMAT CHSLTKIEGV LSGDPLDLKM FEAIGWILEE ATEEETALHN RIMPTVVRPS KQLLPEPTTA GNQEMELFEL PAIYEIGIVR QFPFSSALQR MSVVARTLGE KRMDAYMKGA PEVVASLCKP ETVPVDFEKV LEDYTKQGFR VIALAHRKLE SKLTWHKVQH ISRDAIENNM DFMGLIIMQN KLKQETPAVL EDLHKANIRT VMVTGDNMLT AVSVARDCGM ILPQDKVIIA EALPPKDGKV AKINWHYTDS LSQCSESSAI DSEAIPIKLA HDSLEDLEVT RYHFAMNGKS FSVILEHFQD LVPKLMLHGT VFARMAPDQK TQLVEALQNV DYFVGMCGDG ANDCGALKRA HGGISLSELE ASVASPFTSK TPSISCVPNL IREGRAALMT SFCVFKFMAL YSIIQYFSVT LLYSILSNLG DFQFLFIDLA IILVVVFTMS LNPAWKELVA QRPPSGLISG ALLFSVLSQI VISVGFQSLG FFWVKQYKVC DPNSDVCNTT RSACWNSSHL YNGTELDSCK IQNYENTTVF FISSFQYLTV AVAFSKGKPF RQPCYKNYFF VISVIILYVF ILFIMLHPVA SVDQVLEIMC VPYQWRIYML IIVLINAFVS ITVEESVDRW GKCCLSWALS CRKKTPKAKY MYLAQELRFD PEWPPKPQTT TEAKAVVKEN GSCQIITIA
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Produktmerkmale: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Aufreinigung: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Reinheit: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin-Niveau: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Güteklasse: Crystallography grade

Anwendungsinformationen

Applikationshinweise: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Kommentare:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C.

Haltbarkeit: Unlimited (if stored properly)

Antigendetails

Target: ATP13A3 (ATPase Type 13A3 (ATP13A3))

Andere Bezeichnung: Atp13a3 (ATP13A3 Produkte)

Synonyme: AFURS1 Protein, AU022875 Protein, Gm1745 Protein, Gm541 Protein, Gm542 Protein, ATPase 13A3 Protein, probable cation-transporting ATPase 13A3 Protein, ATPase type 13A3 Protein, ATP13A3 Protein, LOC577790 Protein, LOC100163879 Protein, LOC100202365 Protein, atp13a3 Protein, LOC100561002 Protein, Atp13a3 Protein

Hintergrund: Polyamine-transporting ATPase 13A3 (Putrescine transporting ATPase) (EC 7.6.2.16),FUNCTION: ATP-driven pump involved in endocytosis-dependent polyamine transport. Uses ATP as an energy source to transfer polyamine precursor putrescine from the endosomal compartment to the cytosol. {ECO:0000250|UniProtKB:Q9H7F0}.

Molekulargewicht: 137.5 kDa

UniProt: Q5XF89

Pathways: SARS-CoV-2 Protein Interaktom, Phosphorylierungen bei SARS-CoV-2 Infektion