SEC31A Protein (AA 1-1230) (Strep Tag)

Produktnummer ABIN3151897

Produktdetails

Target: SEC31A (SEC31 Homolog A (SEC31A))

Protein-Typ: Recombinant

Proteineigenschaft: AA 1-1230

Spezies: Maus

Quelle: Tobacco (Nicotiana tabacum)

Aufreinigungstag / Konjugat: Dieses SEC31A Protein ist gelabelt mit Strep Tag.

Applikation: ELISA, Western Blotting (WB), SDS-PAGE (SDS)

Sequenz: MKLKEIDRTA MQAWSPAQNH PIYLATGTSA QQLDATFSTN ASLEIFELDL SDPSLDMKSC ATFSSSHRYH KLIWGPHKMD SKGDVSGVLI AGGENGNIIL YDPSKIIAGD KEVVIAQKDK HTGPVRALDV NIFQTNLVAS GANESEIYIW DLNNFATPMT PGAKTQPPED ISCIAWNRQV QHILASASPS GRATVWDLRK NEPIIKVSDH SNRMHCSGLA WHPDVATQMV LASEDDRLPV IQMWDLRFAS SPLRVLENHA RGILAVAWSM ADPELLLSCG KDAKILCSNP NTGEVLYELP TNTQWCFDIQ WCPRNPAVLS AASFDGRISV YSIMGGSIDG LRQKQVDKLS SSFGNLDPFG TGQPLPPLQI PQQSAQHSIV LPLKKPPKWI RRPVGASFSF GGKLVTFESV AVPLQQGAEQ QRRQPVFISQ VVTEKDFLNR SAQLQHAVQS QGFIGYCQKK IEASQTEFEK NVWSFLKVNF EEDSRGKYLE LLGYRKEDLG QKIALALNKV DGPDVALKDS DQVAQSDGEE SPAAEEQLLG ERIKEEKQEC DFLPSAGGTF NISVSGDIDG LITRALLTGN FESAVDLCLH DNRMADAIIL AIAGGQELLA QTQKKYFAKS QSKITRLITA VVMKNWREIV ESCDLKNWRE ALAAVLTYAK PDEFSALCDL LGTRLEREGD SLLRTQACLC YICAGNVERL VACWTKAQDG SSPLSLQDLI EKVVILRKAV QLTQALDTNT VGALLAEKMS QYASLLAAQG SIAAALAFLP DNTNQPNIVQ LRDRLCKAQG KPVSGQESSQ SPYERQPLSK GRPGPVAGHS QMPRVQTQQY YPHGENPPPP GFIMQGNVIP NPAAPLPTAP GHMPSQLPPY PQPQPYQPAQ QYSFGTGGAA AYRPQQPVAP PASNAYPNTP YISPVASYSG QPQMYTAQQA SSPTSSSAAS FPPPSSGASF QHGGPGAPPS SSAYALPPGT TGTPPAASEL PASQRTENQS FQDQASILEG PQNGWNDPPA LNRVPKKKKM PENFMPPVPI TSPIMNPSGD PQSQGLQQQP STPGPLSSHA SFPQQHLAGG QPFHGVQQPL AQTGMPPSFS KPNTEGAPGA PIGNTIQHVQ ALPTEKITKK PIPEEHLILK TTFEDLIQRC LSSATDPQTK RKLDDASKRL EFLYDKLREQ TLSPTIINGL HSIARSIETR NYSEGLSVHT HIVSTSNFSE TSAFMPVLKV VLSQASKLGV
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Produktmerkmale: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Aufreinigung: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Reinheit: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin-Niveau: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Güteklasse: Crystallography grade

Anwendungsinformationen

Applikationshinweise: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Kommentare:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C.

Haltbarkeit: Unlimited (if stored properly)

Antigendetails

Target: SEC31A (SEC31 Homolog A (SEC31A))

Andere Bezeichnung: Sec31a (SEC31A Produkte)

Synonyme: ABP125 Protein, ABP130 Protein, HSPC334 Protein, SEC31L1 Protein, Sec31l1 Protein, VAP1 Protein, 1810024J13Rik Protein, AU042160 Protein, HSPC275 Protein, cb354 Protein, sec31p Protein, zgc:63547 Protein, SEC31 homolog A, COPII coat complex component Protein, Sec31 homolog A (S. cerevisiae) Protein, SEC31A Protein, Sec31a Protein, sec31a Protein

Hintergrund: Protein transport protein Sec31A (SEC31-like protein 1) (SEC31-related protein A),FUNCTION: Component of the coat protein complex II (COPII) which promotes the formation of transport vesicles from the endoplasmic reticulum (ER) (By similarity). The coat has two main functions, the physical deformation of the endoplasmic reticulum membrane into vesicles and the selection of cargo molecules (By similarity). {ECO:0000250|UniProtKB:O94979, ECO:0000250|UniProtKB:Q9Z2Q1}.

Molekulargewicht: 133.6 kDa

UniProt: Q3UPL0

Pathways: ER-Nucleus Signaling