APLP2 Protein (AA 32-707) (rho-1D4 tag)

Produktnummer ABIN3134846

Produktdetails

Target: APLP2 (Amyloid beta (A4) Precursor-Like Protein 2 (APLP2))

Protein-Typ: Recombinant

Proteineigenschaft: AA 32-707

Spezies: Maus

Quelle: Insektenzellen

Aufreinigungstag / Konjugat: Dieses APLP2 Protein ist gelabelt mit rho-1D4 tag.

Applikation: Western Blotting (WB), SDS-PAGE (SDS), ELISA, Crystallization (Crys)

Sequenz: GYIEALAANA GTGFAVAEPQ IAMFCGKLNM HVNIQTGKWE PDPTGTKSCL GTKEEVLQYC QEIYPELQIT NVMEANQPVN IDSWCRRDKR QCKSHIVIPF KCLVGEFVSD VLLVPDNCQF FHQERMEVCE KHQRWHTLVK EACLTEGLTL YSYGMLLPCG VDQFHGTEYV CCPQTKTVDS DSTMSKEEEE EEEDEEDEEE DYDLDKSEFP TEADLEDFTE AAADEEEEDE EEGEEVVEDR DYYYDPFKGD DYNEENPTEP SSEGTISDKE IVHDVKVPPT PLPTNDVDVY FETSADDNEH ARFQKAKEQL EIRHRNRMDR VKKEWEEAEL QAKNLPKTER QTLIQHFQAM VKALEKEAAS EKQQLVETHL ARVEAMLNDR RRIALENYLA ALQSDPPRPH RILQALRRYV RAENKDRLHT IRHYQHVLAV DPEKAAQMKS QVMTHLHVIE ERRNQSLSLL YKVPYVAQEI QEEIDELLQE QRADMDQFTS SISENPVDVR VSSEESEEIP PFHPLHPFPS LSENEDTQPE LYHPMKKGSG MAEQDGGLIG AEEKVINSKN KMDENMVIDE TLDVKEMIFN AERVGGLEEE PESVGPLRED FSLSSNALIG LLVIAVAIAT VIVISLVMLR KRQYGTISHG IVEVDPMLTP EERHLNKMQN HGYENPTYKY LEQMQI
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Produktmerkmale:

• Made in Germany - from design to production - by highly experienced protein experts.
• Mouse Aplp2 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Aufreinigung: Three step purification of membrane proteins expressed in baculovirus infected SF9 insect cells:

1. Membrane proteins are fractioned by ultracentrifugation and subsequently solubilized with different detergents (detergent screen). Samples are analyzed by Western blot.
2. The best performing detergent is used for solubilization and the proteins are purified via their rho1D4 tag via two rho1D4 antibody columns: one DTT resistant, the other one not. Eluate fractions are analyzed by Western blot.
3. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatograph. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Reinheit: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Sterilität: 0.22 μm filtered

Endotoxin-Niveau: Protein is endotoxin-free.

Güteklasse: Crystallography grade

Anwendungsinformationen

Applikationshinweise: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Kommentare:

Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C.

Haltbarkeit: Unlimited (if stored properly)

Antigendetails

Target: APLP2 (Amyloid beta (A4) Precursor-Like Protein 2 (APLP2))

Andere Bezeichnung: Aplp2 (APLP2 Produkte)

Synonyme: HAI Protein, MT-SP1 Protein, MTSP1 Protein, PRSS14 Protein, SNC19 Protein, TADG15 Protein, TMPRSS14 Protein, APLP-2 Protein, APPH Protein, APPL2 Protein, CDEBP Protein, AI790698 Protein, Apph Protein, WALPLP2 Protein, aplp2 B Protein, APLP2 Protein, si:dkey-11k4.3 Protein, aplp2 A Protein, MGC86389 Protein, suppression of tumorigenicity 14 Protein, amyloid beta precursor like protein 2 Protein, amyloid beta (A4) precursor-like protein 2 Protein, amyloid beta (A4) precursor-like protein 2 S homeolog Protein, amyloid beta (A4) precursor-like protein 2 L homeolog Protein, ST14 Protein, APLP2 Protein, Aplp2 Protein, aplp2.S Protein, aplp2 Protein, aplp2.L Protein

Hintergrund: May play a role in the regulation of hemostasis. The soluble form may have inhibitory properties towards coagulation factors. May interact with cellular G-protein signaling pathways. May bind to the DNA 5'-GTCACATG-3'(CDEI box). Inhibits trypsin, chymotrypsin, plasmin, factor XIA and plasma and glandular kallikrein (By similarity). Modulates the Cu/Zn nitric oxide-catalyzed autodegradation of GPC1 heparan sulfate side chains in fibroblasts. {ECO:0000250, ECO:0000269|PubMed:15677459}.

Molekulargewicht: 78.9 kDa Including tag.

UniProt: Q06335

Pathways: EGFR Signaling Pathway, Transition Metal Ion Homeostasis, Feeding Behaviour