CHL1 Protein (AA 26-1209) (rho-1D4 tag)

Produktnummer ABIN3134630

Produktdetails

Target: CHL1 (Cell Adhesion Molecule with Homology To L1CAM (Close Homolog of L1) (CHL1))

Protein-Typ: Recombinant

Proteineigenschaft: AA 26-1209

Spezies: Maus

Quelle: Insektenzellen

Aufreinigungstag / Konjugat: Dieses CHL1 Protein ist gelabelt mit rho-1D4 tag.

Applikation: Western Blotting (WB), SDS-PAGE (SDS), ELISA, Crystallization (Crys)

Sequenz: EIPLSVQQVP TIVKQSYVQV AFPFDEYFQI ECEAKGNPEP IFSWTKDDKP FDLSDPRIIA ANNSGTFKIP NEGHISHFQG KYRCFASNRL GTAVSEEIEF IVPGVPKFPK EKIEPIDVEE GDSIVLPCNP PKGLPPLHIY WMNIELEHIE QDERVYMSQR GDLYFANVEE NDSRNDYCCF AAFPKLRTIV QKMPMKLTVN SSNSIKQRKP KLLLPPAQMG SLSAKTVLKG DTLLLECFAE GLPTPHIQWS KPGSELPEGR ATIEVHEKTL KIENISYQDR GNYRCTANNL LGKASHDFHV TVEEPPRWKK KPQSAVYSTG SSGILLCEAE GEPQPTIKWR LNGLPIEKHP FPGDFMFPRE ISFTNLLPNH TGVYQCEASN IHGTILANAN IDVIDVIPLI KTKNEENYAT VVGYSAFLHC EYFASPKATV VWEVADETHP LEGDRYHTHE NGTLEIYRTT EEDAGSYSCW VDNAMGKAVI TANLDIRNAT KLRVSPKNPR IPKSHVLELY CESQCDSHLK HSLKLSWSKD GEAFEMNGTE DGRIVIDGAY LTISNITAED QGVYSCSAQT SLDSTSEKTQ VTVLGVPDPP GNLHLSERQN RSVRLSWEAG DDHNSKISEY IVEFEGNREE PGKWEELTRV QGEETDVVLS LAPYVRYQFR VTAVNEVGRS HASLPSDHHE TPPAAPDKNP QNIRVQASQP KEMIIKWEPL KSMEQNGPGL EYKVSWKPQG APEEWEEEIV TNHTLRVMTP TVYAPYDVKV QAINQLGSSP DPQPVTLYSG EDYPSTAPVI QRVDVMNSTL VKVTWSSIPK ETVHGLLRGY QINWWKTKSL LDGRTHPKEV NILRFSGQRN SGMVPSLDPF SEFHLTVLAY NSKGAGPESE PYIFQTPEGV PEQPSFLKVI KVDKDTATLS WGLPKKLNGN LTGYLLQYQI INDTYELGEL NEINVTTPSK SSWHLSNLNS TTKYKFYLRA CTSRGCGKPI SEEGATLGEG SKGIRKITEG VNVTQKIHPV EVLVPGAEHI VHLMTKNWGD NDSIFQDVIE TRGREYAGLY DDISTQGWFI GLMCAIALLT LILLTICFVK RNRGGKYSVK EKEDLHPDPE VQSAKDETFG EYSDSDEKPL KGSLRSLNRN MQPTESADSL VEYGEGDQSI FNEDGSFIGA YTGAKEKGSV ESNGSSTATF PLRA
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Produktmerkmale:

• Made in Germany - from design to production - by highly experienced protein experts.
• Mouse Chl1 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Aufreinigung: Three step purification of membrane proteins expressed in baculovirus infected SF9 insect cells:

1. Membrane proteins are fractioned by ultracentrifugation and subsequently solubilized with different detergents (detergent screen). Samples are analyzed by Western blot.
2. The best performing detergent is used for solubilization and the proteins are purified via their rho1D4 tag via two rho1D4 antibody columns: one DTT resistant, the other one not. Eluate fractions are analyzed by Western blot.
3. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatograph. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Reinheit: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Sterilität: 0.22 μm filtered

Endotoxin-Niveau: Protein is endotoxin-free.

Güteklasse: Crystallography grade

Anwendungsinformationen

Applikationshinweise: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Kommentare:

Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C.

Haltbarkeit: Unlimited (if stored properly)

Antigendetails

Target: CHL1 (Cell Adhesion Molecule with Homology To L1CAM (Close Homolog of L1) (CHL1))

Andere Bezeichnung: Chl1 (CHL1 Produkte)

Synonyme: CALL Protein, L1CAM2 Protein, CHL Protein, CHL1 Protein, Nrln1 Protein, VOPT Protein, A530023M13Rik Protein, AI465420 Protein, LICAM2 Protein, Call Protein, cell adhesion molecule L1 like Protein, chordin-like 1 Protein, cell adhesion molecule L1-like Protein, CHL1 Protein, Chrdl1 Protein, Chl1 Protein

Hintergrund: Extracellular matrix and cell adhesion protein that plays a role in nervous system development and in synaptic plasticity. Both soluble and membranous forms promote neurite outgrowth of cerebellar and hippocampal neurons and suppress neuronal cell death. Plays a role in neuronal positioning of pyramidal neurons as well as in regulation of both the number of interneurons and the efficacy of GABAergic synapses. May play a role in regulating cell migration in nerve regeneration and cortical development. Potentiates integrin-dependent cell migration towards extracellular matrix proteins. Recruits ANK3 to the plasma membrane. {ECO:0000269|PubMed:10022583, ECO:0000269|PubMed:10103075, ECO:0000269|PubMed:12391163, ECO:0000269|PubMed:12721290, ECO:0000269|PubMed:12812975, ECO:0000269|PubMed:14659567, ECO:0000269|PubMed:14761956, ECO:0000269|PubMed:15504324, ECO:0000269|PubMed:16623841}.

Molekulargewicht: 133.5 kDa Including tag.

UniProt: P70232