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L-Gulonolactone Oxidase Protein (Gulonolactone (L-) Oxidase) (AA 2-440) (rho-1D4 tag)

Crystallography grade GULO Spezies: Maus Wirt: Insektenzellen Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. Crys, ELISA, SDS, WB
Produktnummer ABIN3120589
$5,963.83
Zzgl. Versandkosten $45.00 und $20.00 Trockeneis
Lieferung in 50 bis 55 Werktagen
  • Target
    L-Gulonolactone Oxidase (GULO) (Gulonolactone (L-) Oxidase (GULO))
    Protein-Typ
    Recombinant
    Proteineigenschaft
    AA 2-440
    Spezies
    Maus
    Quelle
    Insektenzellen
    Aufreinigungstag / Konjugat
    Dieses L-Gulonolactone Oxidase Protein ist gelabelt mit rho-1D4 tag.
    Applikation
    Crystallization (Crys), ELISA, SDS-PAGE (SDS), Western Blotting (WB)
    Sequenz
    VHGYKGVQFQ NWAKTYGCSP EMYYQPTSVG EVREVLALAR QQNKKVKVVG GGHSPSDIAC TDGFMIHMGK MNRVLQVDKE KKQVTVEAGI LLTDLHPQLD KHGLALSNLG AVSDVTVGGV IGSGTHNTGI KHGILATQVV ALTLMKADGT VLECSESSNA DVFQAARVHL GCLGVILTVT LQCVPQFHLL ETSFPSTLKE VLDNLDSHLK KSEYFRFLWF PHSENVSIIY QDHTNKEPSS ASNWFWDYAI GFYLLEFLLW TSTYLPRLVG WINRFFFWLL FNCKKESSNL SHKIFSYECR FKQHVQDWAI PREKTKEALL ELKAMLEAHP KVVAHYPVEV RFTRGDDILL SPCFQRDSCY MNIIMYRPYG KDVPRLDYWL AYETIMKKFG GRPHWAKAHN CTRKDFEKMY PAFHKFCDIR EKLDPTGMFL NSYLEKVFY
    Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
    Produktmerkmale
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Mouse Gulo Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    Aufreinigung
    Three step purification of membrane proteins expressed in baculovirus infected SF9 insect cells:
    1. Membrane proteins are fractioned by ultracentrifugation and subsequently solubilized with different detergents (detergent screen). Samples are analyzed by Western blot.
    2. The best performing detergent is used for solubilization and the proteins are purified via their rho1D4 tag via two rho1D4 antibody columns: one DTT resistant, the other one not. Eluate fractions are analyzed by Western blot.
    3. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatograph. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    Reinheit
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    Sterilität
    0.22 μm filtered
    Endotoxin-Niveau
    Protein is endotoxin-free.
    Güteklasse
    Crystallography grade
  • Applikationshinweise
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
    Kommentare

    Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Buffer
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    Handhabung
    Avoid repeated freeze-thaw cycles.
    Lagerung
    -80 °C
    Informationen zur Lagerung
    Store at -80°C.
    Haltbarkeit
    Unlimited (if stored properly)
  • Target
    L-Gulonolactone Oxidase (GULO) (Gulonolactone (L-) Oxidase (GULO))
    Andere Bezeichnung
    Gulo
    Synonyme
    5730581M22, AU018375, BC028822, sfx, unh, unhip, gulonolactone (L-) oxidase, L-gulono-gamma-lactone oxidase, Gulo, GULO
    Hintergrund
    Oxidizes L-gulono-1,4-lactone to hydrogen peroxide and L-xylo-hexulonolactone which spontaneously isomerizes to L-ascorbate. {ECO:0000269|PubMed:14962674}.
    Molekulargewicht
    51.5 kDa Including tag.
    UniProt
    P58710
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