PEX2 Protein (AA 1-305) (Strep Tag)

Produktnummer ABIN3120443

Produktdetails

Target: PEX2 (Peroxisomal Biogenesis Factor 2 (PEX2))

Protein-Typ: Recombinant

Proteineigenschaft: AA 1-305

Spezies: Maus

Quelle: Tobacco (Nicotiana tabacum)

Aufreinigungstag / Konjugat: Dieses PEX2 Protein ist gelabelt mit Strep Tag.

Applikation: Western Blotting (WB), SDS-PAGE (SDS), ELISA

Sequenz: MAAREESTQS ANRVLRISQL DALELNKALE QLVWSQFTQC FHGFKPGLLA RFEPEVKAFL WLFLWRFTIY SKNATVGQSV LNIQHKNDSS PNPVYQPPSK NQKLLYAVCT IGGRWLEERC YDLFRNRHLA SFGKAKQCMN FVVGLLKLGE LMNFLIFLQK GKFATLTERL LGIHSVFCKP QNMREVGFEY MNRELLWHGF AEFLIFLLPL INIQKLKAKL SSWCTLCTGA AGHDSTLGSS GKECALCGEW PTMPHTIGCE HVFCYYCVKS SFLFDIYFTC PKCGTEVHSV QPLKAGIQMS EVNAL
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Produktmerkmale: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Aufreinigung: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Reinheit: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin-Niveau: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Güteklasse: Crystallography grade

Anwendungsinformationen

Applikationshinweise: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Kommentare:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C.

Haltbarkeit: Unlimited (if stored properly)

Antigendetails

Target: PEX2 (Peroxisomal Biogenesis Factor 2 (PEX2))

Andere Bezeichnung: Pex2 (PEX2 Produkte)

Synonyme: PAF1 Protein, PBD5A Protein, PBD5B Protein, PMP3 Protein, PMP35 Protein, PXMP3 Protein, RNF72 Protein, ZWS3 Protein, D3Ertd138e Protein, PAF-1 Protein, Pxmp3 Protein, pxmp3 Protein, An18g04150 Protein, DDBDRAFT_0168812 Protein, DDBDRAFT_0238045 Protein, DDB_0168812 Protein, DDB_0238045 Protein, peroxisomal biogenesis factor 2 Protein, RING zinc finger-containing protein Protein, PEX2 Protein, Pex2 Protein, pex2 Protein, ANI_1_1336164 Protein, HCAG_04580 Protein, CC1G_00418 Protein, PTRG_10616 Protein, PAAG_05772 Protein, MGYG_06097 Protein

Hintergrund: Peroxisome biogenesis factor 2 (EC 2.3.2.27) (EC 2.3.2.36) (Peroxin-2) (Peroxisomal membrane protein 3) (Peroxisome assembly factor 1) (PAF-1),FUNCTION: E3 ubiquitin-protein ligase component of a retrotranslocation channel required for peroxisome organization by mediating export of the PEX5 receptor from peroxisomes to the cytosol, thereby promoting PEX5 recycling (By similarity). The retrotranslocation channel is composed of PEX2, PEX10 and PEX12, each subunit contributing transmembrane segments that coassemble into an open channel that specifically allows the passage of PEX5 through the peroxisomal membrane (By similarity). PEX2 also regulates peroxisome organization by acting as a E3 ubiquitin-protein ligase (By similarity). PEX2 ubiquitinates PEX5 during its passage through the retrotranslocation channel: catalyzes monoubiquitination of PEX5 at 'Cys-11', a modification that acts as a signal for PEX5 extraction into the cytosol (By similarity). Required for pexophagy in response to starvation by mediating ubiquitination of peroxisomal proteins, such as PEX5 and ABCD3/PMP70 (By similarity). Also involved in the response to reactive oxygen species (ROS) by mediating 'Lys-48'-linked polyubiquitination and subsequent degradation of PNPLA2/ATGL, thereby regulating lipolysis (PubMed:34903883). {ECO:0000250|UniProtKB:P28328, ECO:0000250|UniProtKB:P32800, ECO:0000269|PubMed:34903883}.

Molekulargewicht: 34.7 kDa

UniProt: P55098

Pathways: Monocarboxylic Acid Catabolic Process