CX3CL1 Protein (AA 2-659) (rho-1D4 tag)

Produktnummer ABIN3120441

Produktdetails

Target: CX3CL1 (Chemokine (C-X3-C Motif) Ligand 1 (CX3CL1))

Protein-Typ: Recombinant

Proteineigenschaft: AA 2-659

Spezies: Maus

Quelle: Insektenzellen

Aufreinigungstag / Konjugat: Dieses CX3CL1 Protein ist gelabelt mit rho-1D4 tag.

Applikation: Western Blotting (WB), SDS-PAGE (SDS), ELISA, Crystallization (Crys)

Sequenz: AAFSKYLTAR NTSLAGAAFL LLCLLHKRRR ALGLHGKKSG KPPLQNNEKE GKKERAVVDK VFLSRLSQIL KIMVPRTFCK ETGYLLLIAV MLVSRTYCDV WMIQNGTLIE SGIIGRSSKD FKRYLFNFIA AMPLISLVNN FLKYGLNELK LCFRVRLTRY LYEEYLQAFT YYKMGNLDNR IANPDQLLTQ DVEKFCNSVV DLYSNLSKPF LDIVLYIFKL TSAIGAQGPA SMMAYLLVSG LFLTRLRRPI GKMTIMEQKY EGEYRYVNSR LITNSEEIAF YNGNKREKQT IHSVFRKLVE HLHNFIFFRF SMGFIDSIIA KYVATVVGYL VVSRPFLDLA HPRHLHSTHS ELLEDYYQSG RMLLRMSQAL GRIVLAGREM TRLAGFTARI TELMQVLKDL NHGRYERTMV SQQEKGIEGA QASPLVPGAG EIINTDNIIK FDHVPLATPN GDILIQDLSF EVRSGANVLI CGPNGCGKSS LFRVLGELWP LFGGRLTKPE RGKLFYVPQR PYMTLGTLRD QVIYPDGKED QKKRGISDQV LKEYLDNVQL GHILEREGGW DSVQDWMDVL SGGEKQRMAM ARLFYHKPQF AILDECTSAV SVDVEDYIYS HCRKVGITLF TVSHRKSLWK HHEYYLHMDG RGNYEFKKIT EDTVEFGS
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Produktmerkmale:

• Made in Germany - from design to production - by highly experienced protein experts.
• Mouse Abcd3 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Aufreinigung: Three step purification of membrane proteins expressed in baculovirus infected SF9 insect cells:

1. Membrane proteins are fractioned by ultracentrifugation and subsequently solubilized with different detergents (detergent screen). Samples are analyzed by Western blot.
2. The best performing detergent is used for solubilization and the proteins are purified via their rho1D4 tag via two rho1D4 antibody columns: one DTT resistant, the other one not. Eluate fractions are analyzed by Western blot.
3. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatograph. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Reinheit: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Sterilität: 0.22 μm filtered

Endotoxin-Niveau: Protein is endotoxin-free.

Güteklasse: Crystallography grade

Anwendungsinformationen

Applikationshinweise: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Kommentare:

Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C.

Haltbarkeit: Unlimited (if stored properly)

Antigendetails

Target: CX3CL1 (Chemokine (C-X3-C Motif) Ligand 1 (CX3CL1))

Andere Bezeichnung: Abcd3 (CX3CL1 Produkte)

Synonyme: ABCD3 Protein, CX3CL1 Protein, DKFZp459K117 Protein, Cx3c Protein, Scyd1 Protein, ABCD-3 Protein, C3Xkine Protein, CXC3 Protein, CXC3C Protein, NTN Protein, NTT Protein, SCYD1 Protein, fractalkine Protein, neurotactin Protein, AB030188 Protein, AI848747 Protein, CX3C Protein, Cxc3 Protein, D8Bwg0439e Protein, ATP binding cassette subfamily D member 3 Protein, C-X3-C motif chemokine ligand 1 Protein, chemokine (C-X3-C motif) ligand 1 Protein, ABCD3 Protein, CX3CL1 Protein, Cx3cl1 Protein, Abcd3 Protein

Hintergrund: Probable transporter involved in the transport of branched-chain fatty acids and C27 bile acids into the peroxisome, the latter function is a crucial step in bile acid biosynthesis. The nucleotide-binding fold acts as an ATP-binding subunit with ATPase activity. {ECO:0000250|UniProtKB:P28288}.

Molekulargewicht: 76.5 kDa Including tag.

UniProt: P55096

Pathways: Synaptic Membrane