TAP2 Protein (AA 1-702) (Strep Tag)

Produktnummer ABIN3133903

Produktdetails

Target: TAP2 (Transporter 2, ATP-Binding Cassette, Sub-Family B (MDR/TAP) (TAP2))

Protein-Typ: Recombinant

Proteineigenschaft: AA 1-702

Spezies: Maus

Quelle: Tobacco (Nicotiana tabacum)

Aufreinigungstag / Konjugat: Dieses TAP2 Protein ist gelabelt mit Strep Tag.

Applikation: Western Blotting (WB), SDS-PAGE (SDS), ELISA

Sequenz: MALSYLRPWV SLLLADMALL GLLQGSLGNL LPQGLPGLWI EGTLRLGVLW GLLKVGELLG LVGTLLPLLC LATPLFFSLR ALVGGTASTS VVRVASASWG WLLAGYGAVA LSWAVWAVLS PAGVQEKEPG QENRTLMKRL LKLSRPDLPF LIAAFFFLVV AVWGETLIPR YSGRVIDILG GDFDPDAFAS AIFFMCLFSV GSSFSAGCRG GSFLFTMSRI NLRIREQLFS SLLRQDLGFF QETKTGELNS RLSSDTSLMS RWLPFNANIL LRSLVKVVGL YFFMLQVSPR LTFLSLLDLP LTIAAEKVYN PRHQAVLKEI QDAVAKAGQV VREAVGGLQT VRSFGAEEQE VSHYKEALER CRQLWWRRDL EKDVYLVIRR VMALGMQVLI LNCGVQQILA GEVTRGGLLS FLLYQEEVGQ YVRNLVYMYG DMLSNVGAAE KVFSYLDRKP NLPQPGILAP PWLEGRVEFQ DVSFSYPRRP EKPVLQGLTF TLHPGTVTAL VGPNGSGKST VAALLQNLYQ PTGGQLLLDG EPLTEYDHHY LHRQVVLVGQ EPVLFSGSVK DNIAYGLRDC EDAQVMAAAQ AACADDFIGE MTNGINTEIG EKGGQLAVGQ KQRLAIARAL VRNPRVLILD EATSALDAQC EQALQNWRSQ GDRTMLVIAH RLHTVQNADQ VLVLKQGRLV EHDQLRDGQD VYAHLVQQRL EA
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Produktmerkmale: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Aufreinigung: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Reinheit: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin-Niveau: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Güteklasse: Crystallography grade

Anwendungsinformationen

Applikationshinweise: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Kommentare:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C.

Haltbarkeit: Unlimited (if stored properly)

Antigendetails

Target: TAP2 (Transporter 2, ATP-Binding Cassette, Sub-Family B (MDR/TAP) (TAP2))

Andere Bezeichnung: Tap2 (TAP2 Produkte)

Synonyme: ABC18 Protein, ABCB3 Protein, APT2 Protein, D6S217E Protein, PSF-2 Protein, PSF2 Protein, RING11 Protein, AI462429 Protein, Abcb3 Protein, Ham-2 Protein, Ham2 Protein, MTP2 Protein, Tap-2 Protein, Y1 Protein, jas Protein, Cim Protein, transporter 2, ATP binding cassette subfamily B member Protein, transporter 2, ATP-binding cassette, sub-family B (MDR/TAP) Protein, TAP2 Protein, Tap2 Protein

Hintergrund: Antigen peptide transporter 2 (APT2) (EC 7.4.2.14) (ATP-binding cassette sub-family B member 3) (Histocompatibility antigen modifier 2),FUNCTION: ABC transporter associated with antigen processing. In complex with TAP1 mediates unidirectional translocation of peptide antigens from cytosol to endoplasmic reticulum (ER) for loading onto MHC class I (MHCI) molecules. Uses the chemical energy of ATP to export peptides against the concentration gradient. During the transport cycle alternates between 'inward-facing' state with peptide binding site facing the cytosol to 'outward-facing' state with peptide binding site facing the ER lumen. Peptide antigen binding to ATP-loaded TAP1-TAP2 induces a switch to hydrolysis-competent 'outward-facing' conformation ready for peptide loading onto nascent MHCI molecules. Subsequently ATP hydrolysis resets the transporter to the 'inward facing' state for a new cycle. As a component of the peptide loading complex (PLC), acts as a molecular scaffold essential for peptide-MHCI assembly and antigen presentation. {ECO:0000250|UniProtKB:Q03519}.

Molekulargewicht: 77.4 kDa

UniProt: P36371

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process