5HT1B Receptor Protein (AA 1-386) (Strep Tag)

Produktnummer ABIN3133566

Produktdetails

Target: 5HT1B Receptor (HTR1B) (Serotonin Receptor 1B (HTR1B))

Protein-Typ: Recombinant

Proteineigenschaft: AA 1-386

Spezies: Maus

Quelle: Tobacco (Nicotiana tabacum)

Aufreinigungstag / Konjugat: Dieses 5HT1B Receptor Protein ist gelabelt mit Strep Tag.

Applikation: Western Blotting (WB), ELISA, SDS-PAGE (SDS)

Sequenz: MEEQGIQCAP PPPAASQTGV PLTNLSHNCS ADGYIYQDSI ALPWKVLLVA LLALITLATT LSNAFVIATV YRTRKLHTPA NYLIASLAVT DLLVSILVMP ISTMYTVTGR WTLGQVVCDF WLSSDITCCT ASIMHLCVIA LDRYWAITDA VEYSAKRTPK RAAIMIVLVW VFSISISLPP FFWRQAKAEE EMLDCFVNTD HVLYTVYSTV GAFYLPTLLL IALYGRIYVE ARSRILKQTP NKTGKRLTRA QLITDSPGST SSVTSINSRA PDVPSESGSP VYVNQVKVRV SDALLEKKKL MAARERKATK TLGIILGAFI VCWLPFFIIS LVMPICKDAC WFHMAIFDFF NWLGYLNSLI NPIIYTMSNE DFKQAFHKLI RFKCAG
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Produktmerkmale: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Aufreinigung: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Reinheit: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin-Niveau: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Güteklasse: Crystallography grade

Anwendungsinformationen

Applikationshinweise: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Kommentare:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C.

Haltbarkeit: Unlimited (if stored properly)

Antigendetails

Target: 5HT1B Receptor (HTR1B) (Serotonin Receptor 1B (HTR1B))

Andere Bezeichnung: Htr1b (HTR1B Produkte)

Synonyme: 5-HT1B Protein, 5-HT1DB Protein, HTR1D2 Protein, HTR1DB Protein, S12 Protein, 5-HT1Db Protein, 5-HT1-LIKE Protein, HTR1B Protein, 5-HT-dro2B Protein, 5-HT1BDro Protein, 5-HT[1B]Dro Protein, 5-HT[[1BDro]] Protein, 5-HT[[1B]] Protein, 5-HT[[1B]]DRO Protein, 5-HT[[1B]]Dro Protein, 5-HT[[dro2B]] Protein, 5HT-R2B Protein, 5HT-dro2B Protein, 5HT-dro[[2B]] Protein, 5HT1B Protein, 5htdro2b Protein, CG15113 Protein, CG30126 Protein, DRO2B Protein, Dm5HTdro2B Protein, Dmel\\CG15113 Protein, Dmel_CG30126 Protein, d5-HT1B Protein, 5-HT-1B Protein, zgc:194666 Protein, zgc:194690 Protein, 5-hydroxytryptamine receptor 1B Protein, 5-hydroxytryptamine (serotonin) receptor 1B Protein, HTR1B Protein, Htr1b Protein, 5-HT1B Protein, htr1b Protein

Hintergrund: 5-hydroxytryptamine receptor 1B (5-HT-1B) (5-HT1B) (Serotonin receptor 1B),FUNCTION: G-protein coupled receptor for 5-hydroxytryptamine (serotonin). Also functions as a receptor for various alkaloids and psychoactive substances. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors, such as adenylate cyclase. Signaling inhibits adenylate cyclase activity. Arrestin family members inhibit signaling via G proteins and mediate activation of alternative signaling pathways. Regulates the release of 5-hydroxytryptamine, dopamine and acetylcholine in the brain, and thereby affects neural activity, nociceptive processing, pain perception, mood and behavior. Besides, plays a role in vasoconstriction of cerebral arteries. {ECO:0000269|PubMed:10651141, ECO:0000269|PubMed:1557407, ECO:0000269|PubMed:16750486, ECO:0000269|PubMed:8091214, ECO:0000269|PubMed:8742487, ECO:0000269|PubMed:9349547}.

Molekulargewicht: 43.1 kDa

UniProt: P28334

Pathways: JAK-STAT Signalweg, cAMP Metabolic Process, Regulation of G-Protein Coupled Receptor Protein Signaling, Feeding Behaviour, S100 Proteine