ABCC3 Protein (AA 1-1523) (Strep Tag)

Produktnummer ABIN3131029

Produktdetails

Target: ABCC3 (ATP-Binding Cassette, Sub-Family C (CFTR/MRP), Member 3 (ABCC3))

Protein-Typ: Recombinant

Proteineigenschaft: AA 1-1523

Spezies: Maus

Quelle: Tobacco (Nicotiana tabacum)

Aufreinigungstag / Konjugat: Dieses ABCC3 Protein ist gelabelt mit Strep Tag.

Applikation: SDS-PAGE (SDS), Western Blotting (WB), ELISA

Sequenz: MDRLCGSGEL GSKFWDSNLS IYTNTPDLTP CFQNSLLAWV PCIYLWAALP CYLFYLRHHQ LGYIVLSWLS RLKTALGVLL WCVSWVDLFY SFHGLIHGSS PAPVFFVTPL VVGITMLLAT LLIQYERLRG VQSSGVLIIF WLLCVICAII PFRSKILSAL AEGKILDPFR FTTFYIYFAL VFCALILSCF KEKPPLFSPE NLDTNPCPEA SAGFFSRLSF WWFTRLAILG YRRPLEDRDL WSLSEEDCSH KVVQRLLEAW QKQQNQASRS QTATAEPKIP GEDAVLLKPR PKSKQPSFLR ALVRTFTSSL LMSACFNLIQ NLLGFVNPQL LSILIRFISD PTAPTWWGFL LAGLMFLSST MQTLILHQYY HCIFVMALRL RTAIIGVIYR KALVITNSVK RESTVGEMVN LMSVDAQRFM DVSPFINLLW SAPLQVILAI YFLWQILGPS ALAGVAVIVL LIPLNGAVSM KMKTYQVKQM KFKDSRIKLM SEILNGIKVL KLYAWEPSFL EQVKGIRQSE LQLLRKGAYL QAISTFIWIC TPFLVTLITL GVYVYVDESN VLDAEKAFVS LSLFNILKIP LNMLPQLISG LTQASVSLKR IQDFLNQNEL DPQCVERKTI SPGYAITIHN GTFTWAQDLP PTLHSLNIQI PKGALVAVVG PVGCGKSSLV SALLGEMEKL EGVVSVKGSV AYVPQQAWIQ NCTLQENVLF GQPMNPKRYQ QALETCALLA DLDVLPGGDQ TEIGEKGINL SGGQRQRVSL ARAVYSDANI FLLDDPLSAV DSHVAKHIFD QVIGPEGVLA GKTRVLVTHG ISFLPQTDFI IVLAGGQVSE MGHYSALLQH DGSFANFLRN YAPDEDQEDH EAALQNANEE VLLLEDTLST HTDLTDNEPA IYEVRKQFMR EMSSLSSEGE VQNRTMPKKH TNSLEKEALV TKTKETGALI KEEIAETGNV KLSVYWDYAK SMGLCTTLSI CLLYGGQSAA AIGANVWLSA WSNDAEEHGQ QNKTSVRLGV YAALGILQGL LVMLSAFTMV VGAIQAARLL HEALLHNKIR SPQSFFDTTP SGRILNRFSK DIYVIDEVLA PTILMLLNSF FTSISTIMVI VASTPLFMVV VLPLAVLYGF VQRFYVATSR QLKRLESISR SPIFSHFSET VTGTSVIRAY GRIQDFKVLS DTKVDNNQKS SYPYIASNRW LGVHVEFVGN CVVLFAALFA VIGRNSLNPG LVGLSVSYAL QVTMALNWMI RMISDLESNI IAVERVKEYS KTKTEAPWVV ESNRAPEGWP TRGMVEFRNY SVRYRPGLEL VLKNVTVHVQ GGEKVGIVGR TGAGKSSMTL CLFRILEAAE GEIVIDGLNV AHIGLHDLRS QLTIIPQDPI LFSGTLRMNL DPFGRYSEED IWRALELSHL NTFVSSQPAG LDFQCAEGGD NLSVGQRQLV CLARALLRKS RVLVLDEATA AIDLETDDLI QGTIRTQFED CTVLTIAHRL NTIMDYNRVL VLDKGVVAEF DSPVNLIAAG GIFYGMAKDA GLA
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Produktmerkmale: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Aufreinigung: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Reinheit: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin-Niveau: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Güteklasse: Crystallography grade

Anwendungsinformationen

Applikationshinweise: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Kommentare:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C.

Haltbarkeit: Unlimited (if stored properly)

Antigendetails

Target: ABCC3 (ATP-Binding Cassette, Sub-Family C (CFTR/MRP), Member 3 (ABCC3))

Andere Bezeichnung: Abcc3 (ABCC3 Produkte)

Synonyme: ABC31 Protein, EST90757 Protein, MLP2 Protein, MOAT-D Protein, MRP3 Protein, cMOAT2 Protein, 1700019L09Rik Protein, Mlp2 Protein, Mrp3 Protein, ATP binding cassette subfamily C member 3 Protein, ATP-binding cassette, sub-family C (CFTR/MRP), member 3 Protein, ABCC3 Protein, Abcc3 Protein

Hintergrund: ATP-binding cassette sub-family C member 3 (EC 7.6.2.-) (EC 7.6.2.2) (EC 7.6.2.3) (Canalicular multispecific organic anion transporter 2) (Multidrug resistance-associated protein 3),FUNCTION: ATP-dependent transporter of the ATP-binding cassette (ABC) family that binds and hydrolyzes ATP to enable active transport of various substrates including many drugs, toxicants and endogenous compound across cell membranes. Transports glucuronide conjugates such as bilirubin diglucuronide, estradiol-17-beta-o-glucuronide and GSH conjugates such as leukotriene C4 (LTC4) (By similarity). Transports also various bile salts (taurocholate, glycocholate, taurochenodeoxycholate-3-sulfate, taurolithocholate- 3-sulfate) (By similarity). Does not contribute substantially to bile salt physiology but provides an alternative route for the export of bile acids and glucuronides from cholestatic hepatocytes (PubMed:15814571, PubMed:16225954). May contribute to regulate the transport of organic compounds in testes across the blood-testis-barrier (By similarity). {ECO:0000250|UniProtKB:O15438, ECO:0000269|PubMed:15814571, ECO:0000269|PubMed:16225954}.

Molekulargewicht: 169.2 kDa

UniProt: B2RX12