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LMAN1 Protein (AA 31-517) (rho-1D4 tag)

Crystallography grade LMAN1 Spezies: Maus Wirt: Insektenzellen Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
Produktnummer ABIN3128789
  • Target Alle LMAN1 Proteine anzeigen
    LMAN1 (Lectin, Mannose-Binding, 1 (LMAN1))
    Protein-Typ
    Recombinant
    Proteineigenschaft
    AA 31-517
    Spezies
    • 4
    • 1
    Maus
    Quelle
    • 3
    • 1
    • 1
    Insektenzellen
    Aufreinigungstag / Konjugat
    Dieses LMAN1 Protein ist gelabelt mit rho-1D4 tag.
    Applikation
    SDS-PAGE (SDS), Western Blotting (WB), ELISA, Crystallization (Crys)
    Sequenz
    DGTGGDAAAP GAAGTQAELP HRRFEYKYSF KGPHLVQSDG TVPFWAHAGN AIPSADQIRI APSLKSQRGS VWTKAKAAFE NWEVEVTFRV TGRGRIGADG LAIWYTENQG LDGPVFGSAD TWNGVGIFFD SFDNDGKKNN PAIVVIGNNG QINYDHQNDG ATQALASCQR DFRNKPYPVR AKITYYQKTL TVMINNGFTP DKNDYEFCAK VENMVIPTQG HFGISAATGG LADDHDVLSF LTFQLTEPGK EPPTAEKDIS EKEKEKYQEE FEHFQQELDK KKEEFQKGHP DLQGQPADDI FESIGDRELR QVFEGQNRIH LEIKQLNRQL DMILDEQRRY VSSLTEEISR RGAGTPGQPG QVSQQELDTV VKSQQEILRQ VNEVKNSMSE TVRLVSGIQH PGSAGVYETT QHFMDIKEHL HVVKRDIDSL AQRSMPSNEK PKCPDLPPFP SCLSTIHFVI FVVVQTVLFV GYIMYRTQQE AAAKKFF
    Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
    Produktmerkmale
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Mouse Lman1 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    Aufreinigung
    Three step purification of membrane proteins expressed in baculovirus infected SF9 insect cells:
    1. Membrane proteins are fractioned by ultracentrifugation and subsequently solubilized with different detergents (detergent screen). Samples are analyzed by Western blot.
    2. The best performing detergent is used for solubilization and the proteins are purified via their rho1D4 tag via two rho1D4 antibody columns: one DTT resistant, the other one not. Eluate fractions are analyzed by Western blot.
    3. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatograph. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    Reinheit
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    Sterilität
    0.22 μm filtered
    Endotoxin-Niveau
    Protein is endotoxin-free.
    Güteklasse
    Crystallography grade
    Top Product
    Discover our top product LMAN1 Protein
  • Applikationshinweise
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
    Kommentare

    Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Buffer
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    Handhabung
    Avoid repeated freeze-thaw cycles.
    Lagerung
    -80 °C
    Informationen zur Lagerung
    Store at -80°C.
    Haltbarkeit
    Unlimited (if stored properly)
  • Target
    LMAN1 (Lectin, Mannose-Binding, 1 (LMAN1))
    Andere Bezeichnung
    Lman1 (LMAN1 Produkte)
    Synonyme
    ERGIC-53 Protein, ERGIC53 Protein, F5F8D Protein, FMFD1 Protein, MCFD1 Protein, MR60 Protein, gp58 Protein, 2610020P13Rik Protein, AI326273 Protein, AU043785 Protein, C730041J05 Protein, P58 Protein, p58 Protein, LMAN1 Protein, cpx-iii Protein, cpxiii Protein, lman1 Protein, wu:fc54c09 Protein, wu:fi36e01 Protein, Xp58 Protein, lman1-a Protein, lectin, mannose binding 1 Protein, lectin, mannose-binding, 1 Protein, complexin 3 Protein, lectin, mannose binding 1 S homeolog Protein, LMAN1 Protein, Lman1 Protein, cplx3 Protein, lman1 Protein, lman1.S Protein
    Hintergrund
    Mannose-specific lectin. May recognize sugar residues of glycoproteins, glycolipids, or glycosylphosphatidyl inositol anchors and may be involved in the sorting or recycling of proteins, lipids, or both. The LMAN1-MCFD2 complex forms a specific cargo receptor for the ER-to-Golgi transport of selected proteins (By similarity). {ECO:0000250}.
    Molekulargewicht
    55.7 kDa Including tag.
    UniProt
    Q9D0F3
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