GRAMD1A Protein (AA 1-722) (Strep Tag)

Produktnummer ABIN3127079

Produktdetails

Target: GRAMD1A (GRAM Domain Containing 1A (GRAMD1A))

Protein-Typ: Recombinant

Proteineigenschaft: AA 1-722

Spezies: Maus

Quelle: Tobacco (Nicotiana tabacum)

Aufreinigungstag / Konjugat: Dieses GRAMD1A Protein ist gelabelt mit Strep Tag.

Applikation: ELISA, SDS-PAGE (SDS), Western Blotting (WB)

Sequenz: MFDTTPHSGR SSPSSSPSLR KRLQLLPPIR PPPASEPEPG TMVEKGSDSS SEKSGVSGTL STQSLGSRNF IRNSKKMQSW YSMLCPTYKQ RNEDFRKLFS KLPEAERLIV DYSCALQREI LLQGRLYLSE NWICFYSNIF RWETTISIQL KEVTCLKKEK TAKLIPNAIQ ICTESEKHFF TSFGARDRCF LLIFRLWQNA LLEKTLSPRE LWHLVHQCYG SELGLTSEDE DYVCPLQLNG LGSPKEVGDV IALSDISPSG AADHSQEPSP VGSRRGRVTP NLSRASSDAD HGAEEDKEEQ TDGLDASSSQ TVTPVAEPLS SEPTPPDGPT SSLGPLDLLS REELLTDTSN SSSSTGEEGD LAALLPDLSG RLLINSVFHM GAERLQQMLF SDSPFLQGFL QQRKFTDVTL SPWSSDSKCH QRRVLTYTIP ISNQLGPKSA SVVETQTLFR RGPQAGGCVV DSEVLTQGIP YQDYFYTAHR YCILGLARNK ARLRVSSEIR YRKQPWSLVK SLIEKNSWSG IEDYFHHLDR ELAKAEKLSL EEGGKDTRGL LSGLRRRKRP LSWRGHRDGP QHPDPDPCTQ TSMHTSGSLS SRFSEPSVDQ GPGAGIPSAL VLISIVLIVL IALNALLFYR LWSLERTAHT FESWHSLALA KGKFPQTATE WAEILALQKH FHSVEVHKWR QILRASVELL DEMKFSLEKL HQGITVPDPP LDTQPQPDDS FP
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Produktmerkmale: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Aufreinigung: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Reinheit: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin-Niveau: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Güteklasse: Crystallography grade

Anwendungsinformationen

Applikationshinweise: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Kommentare:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C.

Haltbarkeit: Unlimited (if stored properly)

Antigendetails

Target: GRAMD1A (GRAM Domain Containing 1A (GRAMD1A))

Andere Bezeichnung: Gramd1a (GRAMD1A Produkte)

Synonyme: KIAA1533 Protein, 1300003M23Rik Protein, AI415002 Protein, AW547240 Protein, D7Bwg0611e Protein, Eg1rvc Protein, RGD1311022 Protein, GRAM domain containing 1A Protein, GRAMD1A Protein, Gramd1a Protein

Hintergrund: Protein Aster-A (GRAM domain-containing protein 1A),FUNCTION: Cholesterol transporter that mediates non-vesicular transport of cholesterol from the plasma membrane (PM) to the endoplasmic reticulum (ER) (PubMed:30220461). Contains unique domains for binding cholesterol and the PM, thereby serving as a molecular bridge for the transfer of cholesterol from the PM to the ER (PubMed:30220461). Plays a crucial role in cholesterol homeostasis and has the unique ability to localize to the PM based on the level of membrane cholesterol (PubMed:30220461). In lipid-poor conditions localizes to the ER membrane and in response to excess cholesterol in the PM is recruited to the endoplasmic reticulum-plasma membrane contact sites (EPCS) which is mediated by the GRAM domain (PubMed:30220461). At the EPCS, the sterol-binding VASt/ASTER domain binds to the cholesterol in the PM and facilitates its transfer from the PM to ER (PubMed:30220461). May play a role in tumor progression (PubMed:27585821). Plays a role in autophagy regulation and is required for biogenesis of the autophagosome. This function in autophagy requires its cholesterol-transfer activity (By similarity). {ECO:0000250|UniProtKB:Q96CP6, ECO:0000269|PubMed:27585821, ECO:0000269|PubMed:30220461}.

Molekulargewicht: 80.7 kDa

UniProt: Q8VEF1