STARD3 Protein (AA 1-446) (Strep Tag)

Produktnummer ABIN3122987

Produktdetails

Target: STARD3 (StAR-Related Lipid Transfer (START) Domain Containing 3 (STARD3))

Protein-Typ: Recombinant

Proteineigenschaft: AA 1-446

Spezies: Maus

Quelle: Tobacco (Nicotiana tabacum)

Aufreinigungstag / Konjugat: Dieses STARD3 Protein ist gelabelt mit Strep Tag.

Applikation: ELISA, Western Blotting (WB), SDS-PAGE (SDS)

Sequenz: MSKRPGDLAC DLERSLPALA SLGTSLSHSQ SLSSHFIPPP LEKRRAISDV RRTFCLFVTF DLLFISLLWI IELNTNTGIR KNLEQEVIHY SFQSSFFDIF VLAFFRFSGL LLGYAVLRLQ HWWVIAVTTL VSSAFLIVKV ILSELLSKGA FGYLLPIVSF VLAWLETWFL DFKVLPQEAE EERWYLAAQA AVARGPLLFS GALSEGQFYS PPESFAGSDN ESDEEVTGKK SFSAQEREYI RQGKEATAVV DQILAQEENW KFERSNEYGD TVYTIEVPFH GKTFILKTFL PCPAELVYQE VILQPERMVL WNKTVTACQI LQRVEDNTLV SYDVSSGAAG GVVSPRDFVN VRRIERRRDR YLSSGIATTH CSKPPTHKYV RGENGPGGFI VLKSANNPRV CTFVWILNTD LKGRLPRYLI HQSLGATMFE FAFHLRQRVG ELGARA
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Produktmerkmale: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Aufreinigung: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Reinheit: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin-Niveau: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Güteklasse: Crystallography grade

Anwendungsinformationen

Applikationshinweise: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Kommentare:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C.

Haltbarkeit: Unlimited (if stored properly)

Antigendetails

Target: STARD3 (StAR-Related Lipid Transfer (START) Domain Containing 3 (STARD3))

Andere Bezeichnung: Stard3 (STARD3 Produkte)

Synonyme: MGC68989 Protein, STARD3 Protein, DKFZp469E1839 Protein, CAB1 Protein, MLN64 Protein, es64 Protein, Mln64 Protein, fc66d12 Protein, mln64 Protein, wu:fc66d12 Protein, StAR related lipid transfer domain containing 3 L homeolog Protein, StAR related lipid transfer domain containing 3 Protein, Protein cab-1 Protein, StAR-related lipid transfer domain containing 3 Protein, START domain containing 3 Protein, StAR-related lipid transfer (START) domain containing 3 Protein, stard3.L Protein, STARD3 Protein, stard3 Protein, cab-1 Protein, Stard3 Protein

Hintergrund: StAR-related lipid transfer protein 3 (Protein ES 64) (Protein MLN 64) (START domain-containing protein 3) (StARD3),FUNCTION: Sterol-binding protein that mediates cholesterol transport from the endoplasmic reticulum to endosomes. The sterol transport mechanism is triggered by phosphorylation of FFAT motif that leads to membrane tethering between the endoplasmic reticulum and late endosomes via interaction with VAPA and VAPB. Acts as a lipid transfer protein that redirects sterol to the endosome at the expense of the cell membrane and favors membrane formation inside endosomes. May also mediate cholesterol transport between other membranes, such as mitochondria membrane or cell membrane. However, such results need additional experimental evidences, probably mainly mediates cholesterol transport from the endoplasmic reticulum to endosomes. Does not activate transcriptional cholesterol sensing. Able to bind other lipids, such as lutein, a xanthophyll carotenoids that form the macular pigment of the retina. {ECO:0000250|UniProtKB:Q14849}.

Molekulargewicht: 50.5 kDa

UniProt: Q61542

Pathways: C21-Steroid Hormone Metabolic Process