Telefon:
+49 (0)241 95 163 153
Fax:
+49 (0)241 95 163 155
E-Mail:
orders@antikoerper-online.de

MMP25 Protein (AA 163-593) (rho-1D4 tag)

Crystallography grade MMP25 Spezies: Maus Wirt: Insektenzellen Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, Crys, ELISA
Produktnummer ABIN3121955
  • Target Alle MMP25 Proteine anzeigen
    MMP25 (Matrix Metallopeptidase 25 (MMP25))
    Protein-Typ
    Recombinant
    Proteineigenschaft
    AA 163-593
    Spezies
    Maus
    Quelle
    • 2
    • 1
    Insektenzellen
    Aufreinigungstag / Konjugat
    Dieses MMP25 Protein ist gelabelt mit rho-1D4 tag.
    Applikation
    SDS-PAGE (SDS), Western Blotting (WB), Crystallization (Crys), ELISA
    Sequenz
    YSLSGSVWKK RTLTWSIRSF SQKSQLSPQI VRTLLSYALA VWATESGLTF QEVNSQYQEP DIIIHFARAY HQDSYPFDGS GGTLAHAFFP GEHPISGDTH FDDEETWTFG STDDNGIDLF AVAVHEFGHA LGLGHSSAPN SIMRPFYQGP VGDPATYRLP QDDRDGLQQL YGRVSQNPNA RPTRKPLVPP PQPPAMPPDS PATPVPDRCE GNFDAVANIR GEIFLFKGPW FWRLQPSGQL VSPRPAGLHR FWEGLPTHVK VIQAAYARPL DGRIILFSGP QFWVFQERQL EGAARPLVEF GLPPGEDVDA VFSWPHNGKT YLIRGQKYWR YDEVAARPDP GYPRALSLWD GAPFAPDDVT ISNTGDTYFF KGTHFWRFAE GSVKAESDSP QPIGPKWLDC PAPNSDPRVT SPPKTTSKTR SCDCHCELNQ A
    Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
    Produktmerkmale
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Mouse Mmp25 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    Aufreinigung
    Three step purification of membrane proteins expressed in baculovirus infected SF9 insect cells:
    1. Membrane proteins are fractioned by ultracentrifugation and subsequently solubilized with different detergents (detergent screen). Samples are analyzed by Western blot.
    2. The best performing detergent is used for solubilization and the proteins are purified via their rho1D4 tag via two rho1D4 antibody columns: one DTT resistant, the other one not. Eluate fractions are analyzed by Western blot.
    3. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatograph. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    Reinheit
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    Sterilität
    0.22 μm filtered
    Endotoxin-Niveau
    Protein is endotoxin-free.
    Güteklasse
    Crystallography grade
    Top Product
    Discover our top product MMP25 Protein
  • Applikationshinweise
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
    Kommentare

    Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Buffer
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    Handhabung
    Avoid repeated freeze-thaw cycles.
    Lagerung
    -80 °C
    Informationen zur Lagerung
    Store at -80°C.
    Haltbarkeit
    Unlimited (if stored properly)
  • Target
    MMP25 (Matrix Metallopeptidase 25 (MMP25))
    Andere Bezeichnung
    Mmp25 (MMP25 Produkte)
    Synonyme
    MMP-25 Protein, MMP20 Protein, MMP20A Protein, MMPL1 Protein, MT-MMP 6 Protein, MT-MMP6 Protein, MT6-MMP Protein, MT6MMP Protein, MTMMP6 Protein, F730048C11Rik Protein, Leukolysin Protein, matrix metallopeptidase 25 Protein, MMP25 Protein, Mmp25 Protein
    Hintergrund
    May activate progelatinase A. {ECO:0000250}.
    Molekulargewicht
    49.2 kDa Including tag.
    UniProt
    Q3U435
Sie sind hier:
Kundenservice