LILRB2 Protein (AA 22-461) (Fc Tag,AVI tag,Biotin)

Produktnummer ABIN3137681

Produktdetails

Target: LILRB2 (Leukocyte Immunoglobulin-Like Receptor, Subfamily B (With TM and ITIM Domains), Member 2 (LILRB2))

Protein-Typ: Recombinant

Biologische Aktivität: Active

Proteineigenschaft: AA 22-461

Spezies: Human

Quelle: HEK-293 Cells

Aufreinigungstag / Konjugat: Dieses LILRB2 Protein ist gelabelt mit Fc Tag,AVI tag,Biotin.

Sequenz: AA 22-461

Spezifität: Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

Produktmerkmale: High Quality Authentic Human LILRB2 Protein

• The production of this recombinant, biotinylated LILRB2 protein is carried out using a proprietary expression platform.
• As expression hosts, the human HEK293 cells have a variety of advantages compared to other cell types.
• Most importantly, the proteins retain their authentic post-translational processing, which often translates into high bioactivity and stability.

High Bioactivity & Detection Sensitivity

• The bioactivity of biotinylated proteins is determined both by the structure of the protein itself, and by the way how biotinylation is performed.
• For every single protein, multiple options of tags and biotinylation methods are tested and the products are evaluated in a variety of binding assays.
• Only those with the best performance are selected for production.

Low Batch-to-Batch Variation

• Products are routinely tested using rigorous quality control measures to ensure consistent performance.
• Newly produced products are subjected to side-by-side comparison with our internal standard in a variety of assays.
• Only those within an acceptable margin of difference are allowed to be released.

Reinheit: >90 % as determined by SDS-PAGE.

Endotoxin-Niveau: Less than 1.0 EU per μg by the LAL method.

Anwendungsinformationen

Kommentare:

Ready-to-use AvitagTM biotinylated protein:
The product is exclusively produced using the AvitagTM technology. Briefly, a unique 15 amino acid peptide, the Avi tag, is introduced into the recombinant protein during expression vector construction. The single lysine residue in the Avi tag is enzymatically biotinylated by the E. Coli biotin ligase BirA.

This single-point enzymatic labeling technique brings many advantages for commonly used binding assays. The biotinylation happens on the lysine residue of Avi tag, and therefore does NOT interfere with the target protein's natural binding activities. In addition, when immobilized on an avidin-coated surface, the protein orientation is uniform because the position of the Avi tag in the protein is precisely controlled.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Buffer: Tris with Glycine, Arginine and NaCl,  pH 7.5

Handhabung: Please avoid repeated freeze-thaw cycles.

Lagerung: -20 °C

Antigendetails

Target: LILRB2 (Leukocyte Immunoglobulin-Like Receptor, Subfamily B (With TM and ITIM Domains), Member 2 (LILRB2))

Andere Bezeichnung: LILRB2 (LILRB2 Produkte)

Hintergrund: Leukocyte immunoglobulin-like receptor subfamily B member 2 (LILRB2) is also known as CD85 antigen-like family member D (CD85d), Immunoglobulin-like transcript 4 (ILT-4), Monocyte / macrophage immunoglobulin-like receptor 10 (MIR-10), which is a member of the the subfamily B class of LIR receptors. LILRB2 is receptor for class I MHC antigens. LILRB2 recognizes a broad spectrum of HLA-A, HLA-B, HLA-C and HLA-G alleles. LILRB2 competes with CD8A for binding to class I MHC antigens. LILRB2 / CD85d inhibits FCGR1A-mediated phosphorylation of cellular proteins and mobilization of intracellular calcium ions.

Molekulargewicht: 76.2 kDa

Pathways: Cellular Response to Molecule of Bacterial Origin