RACGAP1 Protein (AA 1-628) (Strep Tag)

Produktnummer ABIN3137551

Produktdetails

Target: RACGAP1 (Rac GTPase Activating Protein 1 (RACGAP1))

Protein-Typ: Recombinant

Proteineigenschaft: AA 1-628

Spezies: Maus

Quelle: Tobacco (Nicotiana tabacum)

Aufreinigungstag / Konjugat: Dieses RACGAP1 Protein ist gelabelt mit Strep Tag.

Applikation: Western Blotting (WB), SDS-PAGE (SDS), ELISA

Sequenz: MDTTMVNLWT LFEQLVRRME IINEGNESIE FIQVVKDFED FRKKYQRTNQ ELEKFKDLLL KAETGRSALD VKLKHARNQV DVEIKRRQRA EAECAKLEQQ IQLIRDILMC DTSGSIQLSE EQKSALAFLN RGQASSGHAG NNRLSTIDES GSILSDISFD KTDESLDWDS SLVKNFKMKK REKRRSNSRQ FIDGPPGPVK KTCSIGSTVD QANESIVAKT TVTVPSDGGP IEAVSTIETL PSWTRSRGKS GPLQPVNSDS ALNSRPLEPR TDTDNLGTPQ NTGGMRLHDF VSKTVIKPES CVPCGKRIKF GKLSLKCRDC RLVSHPECRD RCPLPCIPPL VGTPVKIGEG MLADFVSQAS PMIPAIVVSC VNEIEQRGLT EAGLYRISGC DRTVKELKEK FLKVKTVPLL SKVDDIHVIC SLLKDFLRNL KEPLLTFWLS KAFMEAAEIT DEDNSTAAMY QAVSELPQAN RDTLAFLMIH LQRVSQSPDT KMDIANLAKV FGPTIVAHTV PNPDPVTMFQ DIKRQLKVVE RLLSLPLEYW NQFMMVDQEN IDSQRGNGNS TPRTPDVKVS LLGPVTTPEF QLVKTPLSSS LSQRLYNLSK STPRFGNKSK SATNLGQQGK FFPAPYLK
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Produktmerkmale: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Aufreinigung: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Reinheit: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin-Niveau: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Güteklasse: Crystallography grade

Anwendungsinformationen

Applikationshinweise: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Kommentare:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C.

Haltbarkeit: Unlimited (if stored properly)

Antigendetails

Target: RACGAP1 (Rac GTPase Activating Protein 1 (RACGAP1))

Andere Bezeichnung: Racgap1 (RACGAP1 Produkte)

Synonyme: CYK4 Protein, HsCYK-4 Protein, ID-GAP Protein, MgcRacGAP Protein, cyk-4 Protein, id-gap Protein, mgcracgap Protein, RACGAP1 Protein, MGC121578 Protein, Gap Protein, RasGAP Protein, Rasa Protein, GAPX Protein, AI227039 Protein, AI327394 Protein, Band25 Protein, GTPase Protein, gtl11 Protein, mKIAA1478 Protein, wu:fb92c08 Protein, zgc:77839 Protein, Rac GTPase activating protein 1 Protein, rac GTPase-activating protein 1 Protein, Rac GTPase activating protein 1 L homeolog Protein, RAS p21 protein activator 1 Protein, Rac GTPase-activating protein 1 Protein, si:ch1073-416j23.1 Protein, RACGAP1 Protein, racgap1 Protein, racgap1.L Protein, racgap1.2.L Protein, racgap1.2 Protein, Rasa1 Protein, Racgap1 Protein, si:ch1073-416j23.1 Protein

Hintergrund: Rac GTPase-activating protein 1 (Male germ cell RacGap) (MgcRacGAP),FUNCTION: Component of the centralspindlin complex that serves as a microtubule-dependent and Rho-mediated signaling required for the myosin contractile ring formation during the cell cycle cytokinesis. Required for proper attachment of the midbody to the cell membrane during cytokinesis. Sequentially binds to ECT2 and RAB11FIP3 which regulates cleavage furrow ingression and abscission during cytokinesis. Plays key roles in controlling cell growth and differentiation of hematopoietic cells through mechanisms other than regulating Rac GTPase activity. Has a critical role in erythropoiesis (By similarity). Also involved in the regulation of growth-related processes in adipocytes and myoblasts. May be involved in regulating spermatogenesis and in the RACGAP1 pathway in neuronal proliferation. Shows strong GAP (GTPase activation) activity towards CDC42 and RAC1 and less towards RHOA. Essential for the early stages of embryogenesis. May play a role in regulating cortical activity through RHOA during cytokinesis. May participate in the regulation of sulfate transport in male germ cells. {ECO:0000250|UniProtKB:Q9H0H5, ECO:0000269|PubMed:10235109, ECO:0000269|PubMed:10493933, ECO:0000269|PubMed:10979956, ECO:0000269|PubMed:11287179}.

Molekulargewicht: 70.2 kDa

UniProt: Q9WVM1

Pathways: Regulation of Actin Filament Polymerization, Myometrial Relaxation and Contraction, Regulation of G-Protein Coupled Receptor Protein Signaling, Signaling of Hepatocyte Growth Factor Receptor