TNIP1 Protein (AA 1-647) (Strep Tag)

Produktnummer ABIN3137515

Produktdetails

Target: TNIP1 (TNFAIP3 Interacting Protein 1 (TNIP1))

Protein-Typ: Recombinant

Proteineigenschaft: AA 1-647

Spezies: Maus

Quelle: Tobacco (Nicotiana tabacum)

Aufreinigungstag / Konjugat: Dieses TNIP1 Protein ist gelabelt mit Strep Tag.

Applikation: ELISA, Western Blotting (WB), SDS-PAGE (SDS)

Sequenz: MEGRGPYRIY DPGGSTPLGE VSAAFERLVE ENTRLKGKMQ GIKMLGELLE ESQMEASRLR QKAEELVKDS ELSPPTSAPS LVSFDDLAEL TGQDTKVQVH PATSTAATTT ATATTGNSME KPEPASKSPS NGASSDFEVV PTEEQNSPET GSHPTNMMDL GPPPPEDSNL KLHLQRLETT LSVCAEEPDH SQLFTHLGRM ALEFNRLASK VHKNEQRTSI LQTLCEQLRQ ENEALKAKLD KGLEQRDLAA ERLREENTEL KKLLMNSSCK EGLCGQPSSP KPEGAGKKGV AGQQQASVMA SKVPEAGAFG AAEKKVKLLE QQRMELLEVN KQWDQHFRSM KQQYEQKITE LRQKLVDLQK QVTELEAERE QKQRDFDRKL LLAKSKIEME ETDKEQLTAE AKELRQKVRY LQDQLSPLTR QREYQEKEIQ RLNKALEEAL SIQASPSSPP AAFGSPEGVG GHLRKQELVT QNELLKQQVK IFEEDFQRER SDRERMNEEK EELKKQVEKL QAQVTLTNAQ LKTLKEEEKA KEALKQQKRK AKASGERYHM EPHPEHVCGA YPYAYPPMPA MVPHHAYKDW SQIRYPPPPV PMEHPPPHPN SRLFHLPEYT WRPPCAGIRN QSSQVMDPPP DRPAEPESAD NDCDGPQ
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Produktmerkmale: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Aufreinigung: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Reinheit: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin-Niveau: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Güteklasse: Crystallography grade

Anwendungsinformationen

Applikationshinweise: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Kommentare:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C.

Haltbarkeit: Unlimited (if stored properly)

Antigendetails

Target: TNIP1 (TNFAIP3 Interacting Protein 1 (TNIP1))

Andere Bezeichnung: Tnip1 (TNIP1 Produkte)

Synonyme: ABIN-1 Protein, NAF1 Protein, VAN Protein, nip40-1 Protein, ABIN Protein, ABIN1 Protein, AU018810 Protein, Naf1 Protein, Nef Protein, im:7141452 Protein, naf1 Protein, zgc:158159 Protein, tnip1 Protein, MGC147597 Protein, TNFAIP3 interacting protein 1 Protein, TNFAIP3 interacting protein 1 S homeolog Protein, TNFAIP3-interacting protein 1 Protein, TNIP1 Protein, Tnip1 Protein, tnip1 Protein, tnip1.S Protein, LOC100078195 Protein

Hintergrund: TNFAIP3-interacting protein 1 (A20-binding inhibitor of NF-kappa-B activation 1) (ABIN) (ABIN-1) (Nef-associated factor 1) (Naf1) (Virion-associated nuclear shuttling protein) (VAN) (mVAN),FUNCTION: Inhibits NF-kappa-B activation and TNF-induced NF-kappa-B-dependent gene expression by regulating TAX1BP1 and A20/TNFAIP3-mediated deubiquitination of IKBKG, proposed to link A20/TNFAIP3 to ubiquitinated IKBKG (By similarity). Involved in regulation of EGF-induced ERK1/ERK2 signaling pathway, blocks MAPK3/MAPK1 nuclear translocation and MAPK1-dependent transcription. Increases cell surface CD4(T4) antigen expression. Involved in the anti-inflammatory response of macrophages and positively regulates TLR-induced activation of CEBPB. Involved in the prevention of autoimmunity, this function implicates binding to polyubiquitin. Involved in leukocyte integrin activation during inflammation, this function is mediated by association with SELPLG and dependent on phosphorylation by SRC-family kinases. {ECO:0000250|UniProtKB:Q15025, ECO:0000269|PubMed:12586352, ECO:0000269|PubMed:21606507, ECO:0000269|PubMed:22011580}.

Molekulargewicht: 73.1 kDa

UniProt: Q9WUU8

Pathways: Activation of Innate immune Response