NRK Protein (AA 1-1455) (Strep Tag)

Produktnummer ABIN3137425

Produktdetails

Target: NRK (Nik Related Kinase (NRK))

Protein-Typ: Recombinant

Proteineigenschaft: AA 1-1455

Spezies: Maus

Quelle: Tobacco (Nicotiana tabacum)

Aufreinigungstag / Konjugat: Dieses NRK Protein ist gelabelt mit Strep Tag.

Applikation: ELISA, Western Blotting (WB), SDS-PAGE (SDS)

Sequenz: MAGPGSWRDK EVTDLGQLPD PTGIFSLDKA IGLGTYGRIF LGIHEKTGSL VAVKVMSARK TPLPEIGRRV RVNKYQKSVG WRYSDEEEDL RTELNLLRKY SFHKNIVTFY GAFFKLNPPG HQHQLWMVME LCAAGSVTDV VRMTRNQSLK EDWIAYICRE ILQGLAHLHA HQVIHRDIKG QNVLLTHDAE VKIVDFGVSA QVSRTNGRRN SFIGTPYWMA PEVIHCDEDP RCSYDYRSDV WSVGITAIEM AEGAPPLCKL QPLEALCVIL REAAPKVKSS GWSRKFQNFM ENCMIKNFLF RPTSGNMLLH PFVHDIKNER RVVESLTKHL TGIIQKREKK GIPVAFEGEE AAKEQYITRR FRGPSCTPEL LRVPTSSRCR PLRVLHGEPP QPRWLPDQED PQDQELQQLQ KAAGVFMPLH SQDNTSKLFP KQVEVAPYLR GAAQVVMPVL VQVEAPPQVS KAAQMLKSLP TQDNKATSPE VQAPVAEGQQ AQHEALETEQ PKDLDQVPEE FQGQDRAPEQ PRQGQAAEQQ QIHNPVPEQP PEEDREPEQA EVQEEAVEPP QAEIEDKEPE VVQVHAQVLL PLLSQNRHVL LPLHLDRQLL IPVGEQNEEV PRAQAWDLEA SRAVGAVQAL IEGLSRDLLR APNAFVTKPL GPLQIFLENL STDGFYTEPE PTQKKKSKVA SLRKAIAKRL RPKRFRAKAL WRLEDFEFSD VETSRRRRHR RWEDIFNQHE EQLRRVENDR EDDSSDNDEV FHSIQAEVQI EPHAANPAGN EVHERSAPMP CNRNRTHRVK FSPSVGEEEP SLEEAQPQQQ QQQPMNIRPR NCLNPQNFQA QSDSSSEEDS PVTRRKSQSS PPYSTIDQKL LIDIHVPDGF KVGKISPPVY LTNEWVGYNA LSEIFWDDWI MPTRPARPPE EDGDYVELYD ADANANGDEE VANGAYEDPR DGANGHDDMN NQLDQANGYE GHGAAGYNGG DVGGNHGAAF NGPRANYPRA GILKNGHNDG RALNRGAFGV FGDNAARAFH GAAGEAGAAF GNHGANRGNG RGNRNREANG RNEENGAFGR DQHVFPEFEH EESDRGTETS DSIALEITSF DGEQNSGRPV SSTTMGFPIG RSSPRGSDFG SDISYNSPIL HVYEKDFSSE VYCGSLWGVN LLLGTQSHLY LMDRSGKAEI VKLIKRRPFR QIQVVEQLNL LITISGKKNR LRVYHLSWLR NKILNNDPKS KKRQKAMRKK EEACKAIDKL IGCEHFSVLQ HEETTYIAVA VKSSIHLFAW APKSFDENTA IKVFPTRDLK PLTVDLAVGS EKTLKIFFSS ANGYHIIDAE SEVMSEVTLP NNNVVILPDC LGLGVMLSLN AEAASEEANE QLLKKILDVW KDIPSSVAFE CTKRITGWDQ KAIEVRSLQS TILENELKRR SIKKLRFLCA RGDKMFFAST LSNDHSRVYL MSLGKLEELH RSYAV
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Produktmerkmale: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Aufreinigung: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Reinheit: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin-Niveau: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Güteklasse: Crystallography grade

Anwendungsinformationen

Applikationshinweise: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Kommentare:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C.

Haltbarkeit: Unlimited (if stored properly)

Antigendetails

Target: NRK (Nik Related Kinase (NRK))

Andere Bezeichnung: Nrk (NRK Produkte)

Synonyme: RGD1566163 Protein, NRK Protein, NESK Protein, AA407323 Protein, AA409510 Protein, AI225895 Protein, AV168563 Protein, AW538185 Protein, Nesk Protein, Nik related kinase Protein, Nrk Protein, NRK Protein

Hintergrund: Nik-related protein kinase (EC 2.7.11.1) (Nck-interacting kinase-like embryo specific kinase) (NESK) (NIK-like embryo-specific kinase),FUNCTION: May phosphorylate cofilin-1 and induce actin polymerization through this process, during the late stages of embryogenesis. Involved in the TNF-alpha-induced signaling pathway. {ECO:0000269|PubMed:10801798, ECO:0000269|PubMed:12837278}.

Molekulargewicht: 163.6 kDa

UniProt: Q9R0G8