COPG2 Protein (AA 1-871) (Strep Tag)

Produktnummer ABIN3137377

Produktdetails

Target: COPG2 (Coatomer Protein Complex, Subunit gamma 2 (COPG2))

Protein-Typ: Recombinant

Proteineigenschaft: AA 1-871

Spezies: Maus

Quelle: Tobacco (Nicotiana tabacum)

Aufreinigungstag / Konjugat: Dieses COPG2 Protein ist gelabelt mit Strep Tag.

Applikation: ELISA, Western Blotting (WB), SDS-PAGE (SDS)

Sequenz: MIKKFDKKDE ESGSGSNPFQ HLEKSAVLQE ARIFNETPIN PRRCLHILTK ILYLLNQGEH FGTMEATEAF FAMTRLFQSN DQTLRRMCYL TIKEMATISE DVIIVTSSLT KDMTGKEDVY RGPAIRALCR ITDGTMLQAV ERYMKQAIVD KVSSVASSAL VSSLHMMKIS YDVVKRWINE AQEAASSDNI MVQYHALGVL YHLRKNDRLA VSKMLNKFTK SGLKSQFAYC MLIRIASRLL KESEDGHESP LFDFIESCLR NKHEMVIYEA ASAIIHLPNC TARELAPAVS VLQLFCSSPK PALRYAAVRT LNKVAMKHPS AVTACNLDLE NLITDSNRSI ATLAITTLLK TGSESSVDRL MKQISSFVSE ISDEFKVVVV QAISALCHKY PRKHSVMMTF LSNMLRDDGG FEYKKAIVDC IISIVEENPE SKEAGLAHLC EFIEDCEHTV LATKILHLLG KEGPRTPVPS KYIRFIFNRV VLENEAVRAA AVSALAKFGA QNESLLPSIL VLLQRCMMDT DDEVRDRATF YLNVLQQRQM ALNATYIFNG LTVSIPGMEK ALHQYTLEPS EKPFDMKSIP LAMAPVFEQK SEITLVTPKP EKLAPSRQDI FQEQLAAIPE FMNLGPLFKS SEPVQLTEAE TEYFVRCVKH MFTDHIVFQF DCTNTLNDQL LEKVTVQMEP SDSYEVLCCI PAPSLPYNQP GICYTLVRLP DEDPTAVAGT FSCTMKFTVR DCDPNTGVPD EDGYDDEYVL EDLEVTVSDH IQKILKPNFA AAWEEVGDAF EKEETFALSS TKTLEEAVNN IITFLGMQPC ERSDKVPENK NSHSLYLAGV YRGGYDLLVR SRLALADGVT MQVTVRSKER TPVDVILASV G
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Produktmerkmale: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Aufreinigung: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Reinheit: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin-Niveau: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Güteklasse: Crystallography grade

Anwendungsinformationen

Applikationshinweise: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Kommentare:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C.

Haltbarkeit: Unlimited (if stored properly)

Antigendetails

Target: COPG2 (Coatomer Protein Complex, Subunit gamma 2 (COPG2))

Andere Bezeichnung: Copg2 (COPG2 Produkte)

Synonyme: 2-COP Protein, cb943 Protein, copg Protein, fi31b06 Protein, wu:fi31b06 Protein, AW227625 Protein, gamma-2-COP Protein, COPG Protein, COPG2 Protein, MGC83366 Protein, MGC79509 Protein, RGD1566215 Protein, coatomer protein complex subunit gamma 2 Protein, coatomer protein complex, subunit gamma 2 Protein, coatomer protein complex subunit gamma 1 Protein, coatomer protein complex subunit gamma 2 S homeolog Protein, coatomer subunit gamma-2 Protein, COPG2 Protein, copg2 Protein, Copg2 Protein, COPG1 Protein, copg2.S Protein, LOC100068740 Protein, LOC100560095 Protein, LOC100594709 Protein

Hintergrund: Coatomer subunit gamma-2 (Gamma-2-coat protein) (Gamma-2-COP),FUNCTION: The coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non-clathrin-coated vesicles, which further mediate biosynthetic protein transport from the ER, via the Golgi up to the trans Golgi network. Coatomer complex is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins. In mammals, the coatomer can only be recruited by membranes associated to ADP-ribosylation factors (ARFs), which are small GTP-binding proteins, the complex also influences the Golgi structural integrity, as well as the processing, activity, and endocytic recycling of LDL receptors (By similarity). {ECO:0000250}.

Molekulargewicht: 97.7 kDa

UniProt: Q9QXK3