Telefon:
+49 (0)241 95 163 153
Fax:
+49 (0)241 95 163 155
E-Mail:
orders@antikoerper-online.de

Naip2 Protein (AA 1-1447) (His tag)

Crystallography grade NAIP2 Spezies: Maus Wirt: Insektenzellen Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. Crys, ELISA, SDS, WB
Produktnummer ABIN3137345
  • Target
    Naip2 (NAIP2) (NLR Family, Apoptosis Inhibitory Protein 2 (NAIP2))
    Protein-Typ
    Recombinant
    Proteineigenschaft
    AA 1-1447
    Spezies
    Maus
    Quelle
    Insektenzellen
    Aufreinigungstag / Konjugat
    Dieses Naip2 Protein ist gelabelt mit His tag.
    Applikation
    Crystallization (Crys), ELISA, SDS-PAGE (SDS), Western Blotting (WB)
    Sequenz
    MAAQGEAVEE IICEFDDDLV SELSTLLRVD ALSVLKRQQE EDHKTRMKMK KGFNSQMRSE AKRLKTFETY DKFRSWTPQE MAAAGFYHTG VKLGVQCFCC SLILFSTRLR KLPIENHKKL RPECEFLLGK DVGNIGKYDI RVKSPEKMLR GDKARYHEEE ARLESFEDWP FYAHGTSPRV LSAAGFVFTG KRDTVQCFSC GGCLGNWEEG DDPWKEHAKW FPKCEFLQSK KSPEEITQYV QSYEGFLHVT GEHFVNSWVR RELPMVSAYC NDSVFANEEL RMDTFKDWPH ESPGAVEALV KAGLFYTGKR DIVQCFSCGG CMEKWAEGDN PIEDHTKFFP NCVFLQTLKS SAEVIPALQS HCALPEAMET TSESNHDDAA AVHSTVVDVS PSEAQELEPA SSLVSVLCRD QDHSEAQGRG CASSGTYLPS TDLGQSEAQW LQEARSLSEQ LRDTYTKATF RHMNLPEVYS SLGTDHLLSC DVSIISKHIS QPVQGSLTIP EVFSNLNSVM CVEGEAGSGK TTFLKRIAFL WASGCCPLLY RFQLVFYLSL SSITPGQELA KIICAQLLGA GGCISEVCLS SIIQQLQHQV LFLLDDYSGL ASLPQALHTL ITKNYLSRTC LLIAVHTNKV RGIRPYLDTS LEIKEFPFYN TVSVLRKLFS HDIMRVRKFI NYFGFHEELQ GIHKTPLFVA AVCTDWFKNP SDQPFQDVAL FKAYMQYLSL KHKGAAKPLQ ATVSSCGQLA LTGLFSSCFE FNSDNLAEAG VDEDEELTTC LMSKFTAQRL RPVYRFLGPL FQEFLAAVRL TELLSSDRQE DQDLGLYYLR QINSPLKAMS IYHTFLKYVS SHPSSKAAPT VVSHLLQLVD EKESLENMSE NEDYMKLHPE ALLWIECLRG LWQLSPESFS LFISENLLRI CLNFAHESNT VAACSPVILQ FLRGRTLDLK VLSLQYFWDH PETLLLLKSI KISLNGNNWV QRIDFSLIEK SFEKVQPPTI DQDYAIAFQP INEVQKNLSE KKHIIKKYED MKHQIPLNIS TGYWKLSPKP YKIPKLEVQV TNTGPADQAL LQVLMEVFSA SQSIEFRLSD SSGFLESIRP ALELSKASVT KCSMSRLELS REDQKLLLTL PTLQSLEVSE TNQLPDQLFH NLHKFLGLKE LCVRLDSKPD VLSVLPGEFP NLHHMEKLSI RTSTESDLSK LVKLIQNSPN LHVFHLKCNF LSNCEPLMTV LASCKKLREI EFSGRCFEAM TFVNILPNFV FLKILNLRDQ QFPDKETSEK FAQALGSLRN LEKLFVPTGD GIHQVAKLIV RQCLQLPCLR VLVFAETLDD DSVLEIAKGA TRGGFQKLEN LDLTLNHKIT EEGYRNFFQV LDNLPNLKNL DISRHIPECI QIQAITVKAL GQCVSRLPSL TRLGMLSWLL DEEDIKVIND VKERHPQSKR LTVHWRWVVP FSPVIQK
    Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
    Produktmerkmale
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Mouse Naip2 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    Aufreinigung
    Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
    1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
    2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    Reinheit
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    Sterilität
    0.22 μm filtered
    Endotoxin-Niveau
    Protein is endotoxin free.
    Güteklasse
    Crystallography grade
  • Applikationshinweise
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
    Kommentare

    Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Buffer
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    Handhabung
    Avoid repeated freeze-thaw cycles.
    Lagerung
    -80 °C
    Informationen zur Lagerung
    Store at -80°C.
    Haltbarkeit
    Unlimited (if stored properly)
  • Target
    Naip2 (NAIP2) (NLR Family, Apoptosis Inhibitory Protein 2 (NAIP2))
    Andere Bezeichnung
    Naip2
    Synonyme
    Birc1b Protein, Naip-rs6 Protein, NLR family, apoptosis inhibitory protein 2 Protein, Naip2 Protein
    Hintergrund
    Sensor component of the NLRC4 inflammasome that specifically recognizes and binds type III secretion system (T3SS) rod proteins such as S.typhimurium (Salmonella) PrgJ and B.thailandensis BsaK from pathogenic bacteria. Association of pathogenic bacteria proteins drives in turn drive assembly and activation of the NLRC4 inflammasome, promoting caspase-1 activation, cytokine production and macrophage pyroptosis. The NLRC4 inflammasome is activated as part of the innate immune response to a range of intracellular bacteria. The NLRC4 inflammasome senses Gram-negative bacteria such as L.pneumophila and P.aeruginosa, enteric pathogens S.typhimurium (Salmonella) and S.flexneri. Prevents motor-neuron apoptosis induced by a variety of signals. {ECO:0000269|PubMed:21874021, ECO:0000269|PubMed:21918512}.
    Molekulargewicht
    165.0 kDa Including tag.
    UniProt
    Q9QUK4
Sie sind hier:
Kundenservice