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GTF2IRD1 Protein (AA 1-1104) (His tag)

Crystallography grade GTF2IRD1 Spezies: Maus Wirt: Insektenzellen Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, Crys, SDS
Produktnummer ABIN3137266
  • Target Alle GTF2IRD1 Proteine anzeigen
    GTF2IRD1 (General Transcription Factor II I Repeat Domain-Containing 1 (GTF2IRD1))
    Protein-Typ
    Recombinant
    Proteineigenschaft
    AA 1-1104
    Spezies
    Maus
    Quelle
    • 2
    • 2
    • 1
    Insektenzellen
    Aufreinigungstag / Konjugat
    Dieses GTF2IRD1 Protein ist gelabelt mit His tag.
    Applikation
    ELISA, Western Blotting (WB), Crystallization (Crys), SDS-PAGE (SDS)
    Sequenz
    MALLGKHCDI PTNGCGSERW NSTFARKDEL INSLVSALDS MCSALSKLNT EVACVAVHNE SVFVMGTEKG RVFLNTRKEL QSDFLRFCRG PLWNDPEAGH PKKVQRCEGG GRSLPRSSLE QCSDVYLLQK MVEEVFDVLY SEAMGRATVV PLPYERLLRE PGLLAVQGLP EGLAFRRPAE YDPKALMAIL EHSHRIRFKL RRPPDDGGQD TKALVEMNGI SLLPKGSRDC GLHGQASKVA PQDLTPTATP SSMANFLYST SMPNHTIREL KQEVPTCPLT PSDLGMGWPV PEPHVPSTQD FSDCCGQTPA GPAGPLIQNV HASKRILFSI VHDKSEKWDP FIKEMEDINT LRECVQILFN SRYAEALGLD HMVPVPYRKI ACDPEAVEIV GIPDKIPFKR PCTYGVPKLK RILEERHSIH FIIKRMFDER IFTGNKFTKD PMKLEPASPP EDTSTEVCRD SMLDLAGTAW SDMSSVSEDC GPGTSGEIAM LRPIKIEPEE LDIIQVTVSD PSPTSEEMTD SLPGHLPSED SGYGMEMPAD KGPSEEPWSE ERPAEESPGD VIRPLRKQVE MLFNTKYAKA IGTSEPVKVP YSKFLMHPEE LFVLGLPEGI SLRRPNCFGI AKLRKILEAS NSIQFVIKRP ELLTDGVKEP VLDTQERDSW DRLVDETPKR QGLQENYNTR LSRIDIANTL REQVQDLFNK KYGEALGIKY PVQVPYKRIK SNPGSVIIEG LPPGIPFRKP CTFGSQNLER ILSVADKIKF TVTRPFQGLI PKPETKILTT GHEAGKTTRP RRLQQDTWQP DEDDANRLGE KVILREQVKE LFNEKYGEAL GLNRPVLVPY KLIRDSPDAV EVKGLPDDIP FRNPNTYDIH RLEKILKARE HVRMVIINQL QPFAEVCNDP KVPEEDDSNK LGKKVILREQ VKELFNEKYG EALGLNRPVL VPYKLIRDSP DAVEVKGLPD DIPFRNPNTY DIHRLEKILK AREHVRMVII NQLQPFGDVC NNAKVPAKDN IPKRKRKRVS EGNSVSSSSS SSSSSSNPES VASTNQISLV VKSRGSELHP NSVWPLPLPR AGPSTAPGTG RHWALRGTQP TTEGQAHPLV LPTR
    Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
    Produktmerkmale
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Mouse Gtf2ird1 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    Aufreinigung
    Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
    1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
    2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    Reinheit
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    Sterilität
    0.22 μm filtered
    Endotoxin-Niveau
    Protein is endotoxin free.
    Güteklasse
    Crystallography grade
    Top Product
    Discover our top product GTF2IRD1 Protein
  • Applikationshinweise
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
    Kommentare

    Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Buffer
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    Handhabung
    Avoid repeated freeze-thaw cycles.
    Lagerung
    -80 °C
    Informationen zur Lagerung
    Store at -80°C.
    Haltbarkeit
    Unlimited (if stored properly)
  • Target
    GTF2IRD1 (General Transcription Factor II I Repeat Domain-Containing 1 (GTF2IRD1))
    Andere Bezeichnung
    Gtf2ird1 (GTF2IRD1 Produkte)
    Synonyme
    Gtf3 Protein, 1700012P16Rik Protein, BEN Protein, Cream1 Protein, ESTM9 Protein, GTF3 Protein, Gtf2il Protein, MusTRD1 Protein, Tg(Alb1-Myc)166.8Sst Protein, WBSCR11 Protein, X83320 Protein, GTF2IRD1 Protein, CREAM1 Protein, MUSTRD1 Protein, RBAP2 Protein, WBS Protein, WBSCR12 Protein, hMusTRD1alpha1 Protein, XBEN Protein, XWBSCR11 Protein, cream1 Protein, gtf3 Protein, mustrd1 Protein, rbap2 Protein, wbs Protein, wbscr11 Protein, wbscr12 Protein, GTF2I repeat domain containing 1 Protein, general transcription factor II I repeat domain-containing 1 Protein, general transcription factor II-I repeat domain-containing protein 1 Protein, GTF2I repeat domain containing 1 S homeolog Protein, GTF2IRD1 Protein, Gtf2ird1 Protein, gtf2ird1 Protein, LOC100461122 Protein, gtf2ird1.S Protein
    Hintergrund
    May be a transcription regulator involved in cell-cycle progression and skeletal muscle differentiation. May repress GTF2I transcriptional functions, by preventing its nuclear residency, or by inhibiting its transcriptional activation. May contribute to slow-twitch fiber type specificity during myogenesis and in regenerating muscles. Binds troponin I slow-muscle fiber enhancer (USE B1). Binds specifically and with high affinity to the EFG sequences derived from the early enhancer of HOXC8. {ECO:0000269|PubMed:11438732}.
    Molekulargewicht
    124.4 kDa Including tag.
    UniProt
    Q9JI57
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