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AFF4 Protein (AA 1-1160) (His tag)

Crystallography grade AFF4 Spezies: Maus Wirt: Insektenzellen Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, Crys, SDS
Produktnummer ABIN3137229
  • Target Alle AFF4 Proteine anzeigen
    AFF4 (AF4/FMR2 Family, Member 4 (AFF4))
    Protein-Typ
    Recombinant
    Proteineigenschaft
    AA 1-1160
    Spezies
    Maus
    Quelle
    • 2
    • 1
    • 1
    Insektenzellen
    Aufreinigungstag / Konjugat
    Dieses AFF4 Protein ist gelabelt mit His tag.
    Applikation
    ELISA, Western Blotting (WB), Crystallization (Crys), SDS-PAGE (SDS)
    Sequenz
    MNREDRNVLR MKERERRNQE IQQGEDAFPP SSPLFAEPYK VTSKEDKLSS RIQSMLGNYD EMKDYIGDRS IPKLVAIPKP AVPTTTDEKA NPNFFEQRHG GSHQSSKWTP VGPAPSTSQS QKRSSALQSG HSSQRSGAGG SGASSSGQRH DRDSYSSSRK KGQHGSEHSK SRSSSPGKPQ AVSSLSSSHS RSHGNDHHSK EHQRSKSPRD PDANWDSPSR GPFSSGQHSS QSFPPSLMSK SSSMLQKPTA YVRPMDGQES VEPKLSSEHY SSQSHGNSMT ELKPSSKAHL TKLKIPSRPL DASVSGDVSC VDEILKEMTH SWPPPLTAIH TPCKTEPSKF PFPTKESQQS NFGPGEQKRY STAKTSNGHQ SKSMLKDDLK LSSSEDSDGE QDCDKTMPRS TPGSNSEPSH HNSEGADNSR DDSSSHSGSE SSSGSDSESE SSSSDSEANE PSQSASPEPE PPPTNKWQLD NWLNKVNPHK VSPASSVDSN IPSSQAYKKE GREQGTASNY TDPGGTKETS SATPGRDSKT IQKGSESGRG RQKSPAQSDS TTQRRTVGKK QPKKPEKSAA EEPRGGLKIE SETPVDMAAS MPSSRHKAAT KGSRKPNIKK ESKSSPRPTA EKKKYKSASK PSQKSREIIE TDTSSSDSDG SESLPPSSQT PKYPESNRTP VKPSSVEEED SFFRQRMFSP MEEKELLSPL SEPDDRYPLI VKIDLNLLTR IPGKPYKETE PPKGEKKNVP EKHSREVQKQ ASEKASNKGK RKHKNDDDTR ASESKKPKTE DKNSSGHKPS SSRESSKQSS TKEKDLLPSP AGPILSKDSK TEHGSRKRTV SQSSSLKSSG TSSKENSGSS SKSSSSSTAK QKKTEGKGPS SSKEAKEKAP NSSSNCPPST PTSESSKPRR TKLAFDDRNY SADHYLQEAK KLKHNADALS DRFEKAVYYL DAVVSFIECG NALEKNAQES KSPFPMYSDT VELIKYTMKL KNYLAPDATA ADKRLTVLCL RCQSLLYLRL FKLKKENALK YSKTLTEHLK NSYSNSQAPS PGLGSKAVGM PSPVSPKLSP GNSGSYSSGG SSASASGSSV TIPQKIHQMA ASYVQVTSNF LYATEIWDQA EQLSKEQKEF FAELDKVMGP LIFNASIMTD LARYTRQGLH WLRQDAKLIS
    Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
    Produktmerkmale
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Mouse Aff4 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    Aufreinigung
    Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
    1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
    2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    Reinheit
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    Sterilität
    0.22 μm filtered
    Endotoxin-Niveau
    Protein is endotoxin free.
    Güteklasse
    Crystallography grade
    Top Product
    Discover our top product AFF4 Protein
  • Applikationshinweise
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
    Kommentare

    Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Buffer
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    Handhabung
    Avoid repeated freeze-thaw cycles.
    Lagerung
    -80 °C
    Informationen zur Lagerung
    Store at -80°C.
    Haltbarkeit
    Unlimited (if stored properly)
  • Target
    AFF4 (AF4/FMR2 Family, Member 4 (AFF4))
    Andere Bezeichnung
    Aff4 (AFF4 Produkte)
    Synonyme
    AFF4 Protein, RA_m002_jsmFBA6Br Protein, AF5Q31 Protein, MCEF Protein, Alf4 Protein, Laf4l Protein, AF4/FMR2 family member 4 L homeolog Protein, AF4/FMR2 family member 4 Protein, AF4/FMR2 family, member 4 Protein, aff4.L Protein, AFF4 Protein, aff4 Protein, Aff4 Protein
    Hintergrund
    Key component of the super elongation complex (SEC), a complex required to increase the catalytic rate of RNA polymerase II transcription by suppressing transient pausing by the polymerase at multiple sites along the DNA. In the SEC complex, AFF4 acts as a central scaffold that recruits other factors through direct interactions with ELL proteins (ELL, ELL2 or ELL3) and the P-TEFb complex. In case of infection by HIV-1 virus, the SEC complex is recruited by the viral Tat protein to stimulate viral gene expression (By similarity). {ECO:0000250}.
    Molekulargewicht
    127.6 kDa Including tag.
    UniProt
    Q9ESC8
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