HIPK3 Protein (AA 1-1192) (His tag)
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- Target Alle HIPK3 Proteine anzeigen
- HIPK3 (Homeodomain Interacting Protein Kinase 3 (HIPK3))
- Protein-Typ
- Recombinant
- Proteineigenschaft
- AA 1-1192
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Spezies
- Maus
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Quelle
- Insektenzellen
- Aufreinigungstag / Konjugat
- Dieses HIPK3 Protein ist gelabelt mit His tag.
- Applikation
- Western Blotting (WB), SDS-PAGE (SDS), ELISA, Crystallization (Crys)
- Sequenz
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MASQVLVYPP YVYQTQSSAF CSVKKLKVEP SGCVFQERTY PQIHVNGRNF GNSHPSTKGS AFQTKIPFTK PRGHSFSLQA GAIVVKDTAG ATKVLAAQAQ QAGVEAPRAV VWRNRLHFLE GPQRCGLKRK SEELENHSGA MQIVDELSIL PAMLQTNMGN PVTVVTATTG SKQNCTSGEG DYQLVQHEVL CSMKNTYEVL DFLGRGTFGQ VVKCWKRGTN EIVAIKILKN HPSYARQGQI EVSILARLST ENADEYNFVR AYECFQHRNH TCLVFEMLEQ NLYDFLKQNK FSPLPLKVIR PVLQQVATAL KKLKSLGLIH ADLKPENIML VDPVRQPYRV KVIDFGSASH VSKTVCSTYL QSRYYRAPEI ILGLPFCEAI DMWSLGCVIA ELFLGWPLYP GALEYDQIRY ISQTQGLPGE QLLNVGTKST RFFCRETDMS HSGWRLKTLE EHEAETGMKS KEARKYIFNS LDDIVHVNTV MDLEGGDLLA EKADRREFVN LLKKMLLIDA DLRITPIETL NHPFVNMKHL LDFPHSNHVK SCFHIMDICK SPSSCETNNH SKMSLLRPVA SNGTAALAAN FTKVGTLRSQ ALTTSAHSVV HHGIPLQAGT AQFGCGDAFH QTLIICPPAI QGIPAAHGKP TSYSIRVDNT VPLVTQAPAV QPLQIRPGVL SQQTWSGRTQ QMLIPAWQQV TPMAPAAATL TSEGMAGSQR LGDWGKMIPH SNHYNSVMPP PLLTNQITLS APQPISVGIA HVVWPQPATT KKNKLCQNRS NSLQNTNIPH SAFISPKIIS GKEVEEVSCV DTQDNHTSEG EAGTCREASV RQDSSVSDKQ RQTIIIADSP SPAVSVITIS SDSDDEETSP RPSLRECKGS LDCEACQSTL NIDRMCSLSS PDSTLSTSSS GQSSPSPCKR PNSMSDDEQE SGCETVDGSP TSDSSGHDSP FAENSFVEDA HQNTELGTCA GPEAKPAVGT AVEPPVGRES GLSVDEHMAN TDSTCQPLRK GQPAPGKLHQ PPALGARQQK PAAAFPQQHL NLSQVQHFGT GHQEWNGNFG HRRQQAYIPT SVTSNPFTLS HGSPNHTAVH AHLAGSTHLG GQPTLLPYPS SASLSSAAPV AHLLASPCTS RPMLQHPTYN ISHPSGIVHQ VPVGINPRLL PSPTIHQTQY KPIFPPHSYI AASPAYTGFP LSPTKLSQYP YM
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us. - Produktmerkmale
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- Made in Germany - from design to production - by highly experienced protein experts.
- Mouse Hipk3 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).
When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.
The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- Aufreinigung
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Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
- In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- Reinheit
- >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- Sterilität
- 0.22 μm filtered
- Endotoxin-Niveau
- Protein is endotoxin free.
- Güteklasse
- Crystallography grade
- Top Product
- Discover our top product HIPK3 Protein
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- Applikationshinweise
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
- Kommentare
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Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Liquid
- Buffer
- 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
- Handhabung
- Avoid repeated freeze-thaw cycles.
- Lagerung
- -80 °C
- Informationen zur Lagerung
- Store at -80°C.
- Haltbarkeit
- Unlimited (if stored properly)
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- Target
- HIPK3 (Homeodomain Interacting Protein Kinase 3 (HIPK3))
- Andere Bezeichnung
- Hipk3 (HIPK3 Produkte)
- Synonyme
- HIPK3 Protein, pky Protein, yak1 Protein, dyrk6 Protein, fist3 Protein, MGC145460 Protein, DYRK6 Protein, FIST3 Protein, PKY Protein, YAK1 Protein, ANPK Protein, homeodomain interacting protein kinase 3 Protein, homeodomain interacting protein kinase 3 L homeolog Protein, HIPK3 Protein, hipk3.L Protein, hipk3 Protein, Hipk3 Protein
- Hintergrund
- Serine/threonine-protein kinase involved in transcription regulation, apoptosis and steroidogenic gene expression. Phosphorylates JUN and RUNX2. Seems to negatively regulate apoptosis by promoting FADD phosphorylation. Enhances androgen receptor-mediated transcription. May act as a transcriptional corepressor for NK homeodomain transcription factors. The phosphorylation of NR5A1 activates SF1 leading to increased steroidogenic gene expression upon cAMP signaling pathway stimulation. In osteoblasts, supports transcription activation: phosphorylates RUNX2 that synergizes with SPEN/MINT to enhance FGFR2-mediated activation of the osteocalcin FGF-responsive element (OCFRE). {ECO:0000269|PubMed:11034606, ECO:0000269|PubMed:17210646, ECO:0000269|PubMed:20484411}.
- Molekulargewicht
- 131.0 kDa Including tag.
- UniProt
- Q9ERH7
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