Acsl3 Protein (AA 42-720) (His tag)

Produktnummer ABIN3137063

Produktdetails

Target: Acsl3 (Acyl-CoA Synthetase Long-Chain Family Member 3 (Acsl3))

Protein-Typ: Recombinant

Proteineigenschaft: AA 42-720

Spezies: Maus

Quelle: Insektenzellen

Aufreinigungstag / Konjugat: Dieses Acsl3 Protein ist gelabelt mit His tag.

Applikation: ELISA, Western Blotting (WB), SDS-PAGE (SDS), Crystallization (Crys)

Sequenz: YFLCESKQEK PNQIKAKPVS SKPDSAYRSI NSVDGLASVL YPGCDTLDKV FMYAKNKFKN KRLLGTREIL NEEDEIQPNG KIFKKVILGH YNWLSYEDVF IRALDFGNGL QMLGQKPKAN IAIFCETRAE WMIAAQACFM YNFQLVTLYA TLGGPAIVHG LNETEVTNII TSKELLQTKL KDIVSLVPRL RHIITVDGKP PTWSEFPKGV IVHTMAAVQA LGVKANVEKK AHSKPLPSDI AVIMYTSGST GIPKGVMISH SNIIASITGM ARRIPRLGEE DVYIGYLPLA HVLELSAELV CLSHGCRIGY SSPQTLADQS SKIKKGSKGD TSVLKPTLMA AVPEIMDRIY KNVMNKVNEM SAFQRNLFIL AYNYKMEQIS KGCSTPLCDR FVFRNVRRLL GGNIRLLLCG GAPLSATTQR FMNICFCCPV GQGYGLTEST GAGTITEVWD YNTGRVGAPL VCCEIKLKNW EEGGYFNTDK PHPRGEILIG GQNVTMGYYK NEAKTKTDFF EDENGQRWLC TGDIGEFDPD GCLKIIDRKK DLVKLQAGEY VSLGKVEAAL KNLPLIDNIC AYANSYHSYV IGFVVPNQKE LTELARTKGF KGTWEELCNS SEMENEVLKV LSEAAISASL EKFEIPLKIR LSPDPWTPET GLVTDAFKLK RKELKTHYQA DIERMYGRK
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Produktmerkmale:

• Made in Germany - from design to production - by highly experienced protein experts.
• Mouse Acsl3 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Aufreinigung: Two step purification of proteins expressed in baculovirus infected SF9 insect cells:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Reinheit: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Sterilität: 0.22 μm filtered

Endotoxin-Niveau: Protein is endotoxin free.

Güteklasse: Crystallography grade

Anwendungsinformationen

Applikationshinweise: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Kommentare:

Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C.

Haltbarkeit: Unlimited (if stored properly)

Antigendetails

Target: Acsl3 (Acyl-CoA Synthetase Long-Chain Family Member 3 (Acsl3))

Andere Bezeichnung: Acsl3 (Acsl3 Produkte)

Synonyme: ACSL3 Protein, acsl3 Protein, fa07a08 Protein, im:7155129 Protein, si:dkey-20f20.5 Protein, wu:fa07a08 Protein, wu:fb34c03 Protein, si:dkeyp-109h9.2 Protein, ACS3 Protein, FACL3 Protein, PRO2194 Protein, 2610510B12Rik Protein, Acs3 Protein, C85929 Protein, Facl3 Protein, Pro2194 Protein, F15H21.7 Protein, F15H21_7 Protein, long-chain acyl-CoA synthetase 3 Protein, acyl-CoA synthetase long-chain family member 3 Protein, acyl-CoA synthetase long chain family member 3 Protein, acyl-CoA synthetase long chain family member 3b Protein, acyl-CoA synthetase long chain family member 3a Protein, AcsL3 Protein, AMP-dependent synthetase and ligase family protein Protein, ACSL3 Protein, acsl3b Protein, acsl3a Protein, acsL3 Protein, acsl3 Protein, Acsl3 Protein, LACS3 Protein

Hintergrund: Acyl-CoA synthetases (ACSL) activates long-chain fatty acids for both synthesis of cellular lipids, and degradation via beta-oxidation. ACSL3 has mainly an anabolic role in energy metabolism (By similarity). Required for the incorporation of fatty acids into phosphatidylcholine, the major phospholipid located on the surface of VLDL (very low density lipoproteins) (By similarity). Mediates hepatic lipogenesis (By similarity). Preferentially uses myristate, laurate, arachidonate and eicosapentaenoate as substrates (By similarity). {ECO:0000250}.

Molekulargewicht: 76.6 kDa Including tag.

UniProt: Q9CZW4

Pathways: SARS-CoV-2 Protein Interaktom