ERC1 Protein (AA 1-1120) (Strep Tag)

Produktnummer ABIN3136965

Produktdetails

Target: ERC1 (ELKS/RAB6-Interacting/CAST Family Member 1 (ERC1))

Protein-Typ: Recombinant

Proteineigenschaft: AA 1-1120

Spezies: Maus

Quelle: Tobacco (Nicotiana tabacum)

Aufreinigungstag / Konjugat: Dieses ERC1 Protein ist gelabelt mit Strep Tag.

Applikation: ELISA, Western Blotting (WB), SDS-PAGE (SDS)

Sequenz: MYGSARSVGK VEPSSQSPGR SPRLPRSPRL GHRRTNSTGG SSGNSVGGGS GKTLSMENIQ SLNAAYATSG PMYLSDHENV GAETPKSTMT LGRSGGRLPY GVRMTAMGSS PNIASSGVAS DTIAFGEHHL PPVSMASTVP HSLRQARDNT IMDLQTQLKE VLRENDLLRK DVEVKESKLS SSMNSIKTFW SPELKKERAL RKDEASKITI WKEQYRVVQE ENQHMQMTIQ ALQDELRIQR DLNQLFQQDS SSRTGEPCVA ELTEENFQRL HAEHERQAKE LFLLRKTLEE MELRIETQKQ TLNARDESIK KLLEMLQSKG LSAKATEEDH ERTRRLAEAE MHVHHLESLL EQKEKENNML REEMHRRFEN APDSAKTKAL QTVIEMKDSK ISSMERGLRD LEEEIQMLKS NGALSSEERE EEMKQMEVYR SHSKFMKNKV EQLKEELSSK DAQGEELKKR AAGLQSEIGQ VKQELSRKDT ELLALQTKLE TLTNQFSDSK QHIEVLKESL TAKEQRAAIL QTEVDALRLR LEEKETMLNK KTKQIQDMAE EKGTQAGEIH DLKDMLDVKE RKVNVLQKKI ENLQEQLRDK EKQMSSLKER VKSLQADTTN TDTALTTLEE ALADKERTIE RLKEQRDRDE REKQEEIDTY KKDLKDLREK VSLLQGDLSE KEASLLDIKE HASSLASSGL KKDSRLKTLE IALEQKKEEC LKMESQLKKA HEATLEARAS PEMSDRIQQL EREISRYKDE SSKAQTEVDR LLEILKEVEN EKNDKDKKIA ELESLTSRQV KDQNKKVANL KHKEQVEKKK SAQMLEEARR REDSLSDSSQ QLQDSLRKKD DRIEELEEAL RESVQITAER EMVLAQEESA RTNAEKQVEE LLMAMEKVKQ ELESMKAKLS STQQSLAEKE THLTNLRAER RKHLEEVLEM KQEALLAAIS EKDANIALLE LSSSKKKTQE EVAALKREKD RLVQQLKQQT QNRMKLMADN YEDDHFRSSR SNQTNHKPSP DQIIQPLLEL DQNRSKLKLY IGHLTALCHD RDPLILRGLT PPASYNADGE QAAWENELQQ MTQEQLQNEL EKVEGDNAEL QEFANTILQQ IADHCPDILE QVVNALEESS
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Produktmerkmale: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Aufreinigung: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Reinheit: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin-Niveau: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Güteklasse: Crystallography grade

Anwendungsinformationen

Applikationshinweise: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Kommentare:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C.

Haltbarkeit: Unlimited (if stored properly)

Antigendetails

Target: ERC1 (ELKS/RAB6-Interacting/CAST Family Member 1 (ERC1))

Andere Bezeichnung: Erc1 (ERC1 Produkte)

Synonyme: Cast2 Protein, ELKS Protein, RAB6IP2 Protein, 4930404L01Rik Protein, 5033405M01Rik Protein, 9630025C19Rik Protein, B430107L16Rik Protein, RAB6IP2A Protein, RAB6IP2B Protein, Rab6ip2 Protein, mKIAA1081 Protein, CAST Protein, ERC Protein, ERC1b Protein, Elks Protein, ELKS/RAB6-interacting/CAST family member 1 Protein, ELKS/Rab6-interacting/CAST family member 1 Protein, ERC1 Protein, erc1 Protein, LOC100540472 Protein, Erc1 Protein

Hintergrund: ELKS/Rab6-interacting/CAST family member 1 (ERC-1) (CAZ-associated structural protein 2) (CAST2) (Rab6-interacting protein 2),FUNCTION: Regulatory subunit of the IKK complex. Probably recruits IkappaBalpha/NFKBIA to the complex (By similarity). May be involved in the organization of the cytomatrix at the nerve terminals active zone (CAZ) which regulates neurotransmitter release. May be involved in vesicle trafficking at the CAZ. May be involved in Rab-6 regulated endosomes to Golgi transport. {ECO:0000250}.

Molekulargewicht: 128.3 kDa

UniProt: Q99MI1

Pathways: SARS-CoV-2 Protein Interaktom, Phosphorylierungen bei SARS-CoV-2 Infektion