FRMD4B Protein (AA 1-1035) (Strep Tag)

Produktnummer ABIN3136859

Produktdetails

Target: FRMD4B (FERM Domain Containing 4B (FRMD4B))

Protein-Typ: Recombinant

Proteineigenschaft: AA 1-1035

Spezies: Maus

Quelle: Tobacco (Nicotiana tabacum)

Aufreinigungstag / Konjugat: Dieses FRMD4B Protein ist gelabelt mit Strep Tag.

Applikation: ELISA, SDS-PAGE (SDS), Western Blotting (WB)

Sequenz: MASVFMCGVE DLLFSGSRFV WNLTVSTLRR WYTERLRACH QVLRTWCGLR DVYQMTEGRH CQVHLLDDRR LELLVQPKLL SRELLDLVAS HFNLKEKEYF GITFIDDTGQ ENWLQLDHRV LEHDLPKKPG PTLLHFAVRF YIESISFLKD KNTVELFFLN AKACVHKGQI EVDSETIFKL AALVLQESKG DYTSDENARK DLKTLPVFPT KTLQEHPSLA YCEDRVIEHY LKIKGLTRGQ AVVQYMKIVE ALPTYGVHYY AVKDKQGLPW WLGISYKGIG QYDLQDKVKP RKLFQWKQLE NLYFREKKFA VEVHDPRRIS VSRRTFGQSG LFVQTWYANS SLIKSIWVMA ISQHQFYLDR KQSKAKIPSA RSLDDIAMDL TETGTQRGSK LVTLEAKSQF IMASNGSLIS SGSQDSEGME EQKREKILEL KKKEKLLQEK LLQKVEELKK ICLREAELTG RMPKEYPLNI GEKPPQVRRR VGTTFKLDDN LLPTEEDPAL QELESNFLIQ QKLVEAAKKL ASEPDLCKTV KKKRKQDYTD AVKRLQEIEN SINEYRIRCG KKPSQKAAVV PPEDIIPSES SSLSDTTTYD DPNDSFTLAG QRPSSVPHSP RILPPKSLGI ERIHFRKSSI NEQFMDTRHS REMLSTHSSP YKTLERRPQG GRSMPTTPVL TRNAYSSSHL EPDSSSQHCR QRSGSLESQS HLLSEMDSDK PFFTLSKSQR SSSTEILDDG SSYTSQSSSE YYCVTPAASP YYTTQTLDTR ARGRRRSKKH SVSTSNSGSM PNLAQKDPLR NGVYSKGQDP PPSGYYIAGY PPYAECDLYY SGGYVYENDT EGQYSVNPSY RSSAHYGYDR QRDYSRSFHE DEVDRVPHNP YATLRLPRKA AVKSEHITKN IHKALVAEHL RGWYQRASGQ KDQGHSPQTS FDSDRGSQRC LGFAGLQVPC SPSSRASSYS SVSSTNASGN WRTQLTIGLS EYENPVHSPY TSYYGSIYNP LSSPSRQYAE TTPLDGTDGS QLEDNLEGSE QRLFWHEDSK PGTLV
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Produktmerkmale: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Aufreinigung: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Reinheit: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin-Niveau: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Güteklasse: Crystallography grade

Anwendungsinformationen

Applikationshinweise: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Kommentare:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C.

Haltbarkeit: Unlimited (if stored properly)

Antigendetails

Target: FRMD4B (FERM Domain Containing 4B (FRMD4B))

Andere Bezeichnung: Frmd4b (FRMD4B Produkte)

Synonyme: FRMD4B Protein, 6030440G05Rik Protein, GRSP1 Protein, C87375 Protein, GOBLIN Protein, R74720 Protein, mKIAA1013 Protein, Grsp1 Protein, Nitzin Protein, FERM domain containing 4B Protein, FRMD4B Protein, frmd4b Protein, Frmd4b Protein

Hintergrund: FERM domain-containing protein 4B (GRP1-binding protein GRSP1) (Golgi-associated band 4.1-like protein) (GOBLIN),FUNCTION: Member of GRP1 signaling complexes that are acutely recruited to plasma membrane ruffles in response to insulin receptor signaling. May function as a scaffolding protein that regulates epithelial cell polarity by connecting ARF6 activation with the PAR3 complex (PubMed:20080746). Plays a redundant role with FRMD4A in epithelial polarization (PubMed:20080746). {ECO:0000269|PubMed:20080746}.

Molekulargewicht: 118.0 kDa

UniProt: Q920B0