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BAAT Protein (AA 1-420) (His tag)

Crystallography grade BAAT Spezies: Maus Wirt: Insektenzellen Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, Crys, SDS
Produktnummer ABIN3136808
  • Target Alle BAAT Proteine anzeigen
    BAAT (Bile Acid CoA: Amino Acid N-Acyltransferase (Glycine N-Choloyltransferase) (BAAT))
    Protein-Typ
    Recombinant
    Proteineigenschaft
    AA 1-420
    Spezies
    Maus
    Quelle
    • 1
    • 1
    • 1
    Insektenzellen
    Aufreinigungstag / Konjugat
    Dieses BAAT Protein ist gelabelt mit His tag.
    Applikation
    ELISA, Western Blotting (WB), Crystallization (Crys), SDS-PAGE (SDS)
    Sequenz
    MAKLTAVPLS ALVDEPVHIQ VTGLAPFQVV CLQASLKDEK GNLFSSQAFY RASEVGEVDL EHDPSLGGDY MGVHPMGLFW SLKPEKLLGR LIKRDVMNSP YQIHIKACHP YFPLQDIVVS PPLDSLTLER WYVAPGVKRI QVKESRIRGA LFLPPGEGPF PGVIDLFGGA GGLMEFRASL LASRGFATLA LAYWNYDDLP SRLEKVDLEY FEEGVEFLLR HPKVLGPGVG ILSVCIGAEI GLSMAINLKQ IRATVLINGP NFVSQSPHVY HGQVYPPVPS NEEFVVTNAL GLVEFYRTFQ ETADKDSKYC FPIEKAHGHF LFVVGEDDKN LNSKVHANQA IAQLMKNGKK NWTLLSYPGA GHLIEPPYTP LCQASRMPIL IPSLSWGGEV IPHAAAQEHS WKEIQKFLKQ HLLPDLSSQL
    Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
    Produktmerkmale
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Mouse Baat Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    Aufreinigung
    Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
    1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
    2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    Reinheit
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    Sterilität
    0.22 μm filtered
    Endotoxin-Niveau
    Protein is endotoxin free.
    Güteklasse
    Crystallography grade
    Top Product
    Discover our top product BAAT Protein
  • Applikationshinweise
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
    Kommentare

    Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Buffer
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    Handhabung
    Avoid repeated freeze-thaw cycles.
    Lagerung
    -80 °C
    Informationen zur Lagerung
    Store at -80°C.
    Haltbarkeit
    Unlimited (if stored properly)
  • Target
    BAAT (Bile Acid CoA: Amino Acid N-Acyltransferase (Glycine N-Choloyltransferase) (BAAT))
    Andere Bezeichnung
    Baat (BAAT Produkte)
    Synonyme
    BACAT Protein, BAT Protein, AI118337 Protein, AI158864 Protein, kan-1 Protein, BAAT Protein, bile acid-CoA:amino acid N-acyltransferase Protein, Bile acid-CoA:amino acid N-acyltransferase Protein, bile acid-Coenzyme A: amino acid N-acyltransferase Protein, bile acid CoA:amino acid N-acyltransferase Protein, BAAT Protein, RPIC_RS10270 Protein, Bcav_2277 Protein, Rpic12D_1765 Protein, Baat Protein, LOC481635 Protein, LOC100054567 Protein, LOC786798 Protein
    Hintergrund
    Involved in bile acid metabolism. In liver hepatocytes catalyzes the second step in the conjugation of C24 bile acids (choloneates) to taurine before excretion into bile canaliculi. The major components of bile are cholic acid and chenodeoxycholic acid. In a first step the bile acids are converted to an acyl-CoA thioester, either in peroxisomes (primary bile acids deriving from the cholesterol pathway), or cytoplasmic at the endoplasmic reticulum (secondary bile acids). May catalyze the conjugation of primary or secondary bile acids, or both. The conjugation increases the detergent properties of bile acids in the intestine, which facilitates lipid and fat-soluble vitamin absorption. In turn, bile acids are deconjugated by bacteria in the intestine and are recycled back to the liver for reconjugation (secondary bile acids). May also act as an acyl-CoA thioesterase that regulates intracellular levels of free fatty acids. In vitro, catalyzes the hydrolysis of long- and very long-chain saturated acyl-CoAs to the free fatty acid and coenzyme A (CoASH), and conjugates glycine to these acyl-CoAs. {ECO:0000250|UniProtKB:Q14032}.
    Molekulargewicht
    47.4 kDa Including tag.
    UniProt
    Q91X34
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