REST Protein (AA 1-1082) (Strep Tag)

Produktnummer ABIN3136764

Produktdetails

Target: REST (RE1-Silencing Transcription Factor (REST))

Protein-Typ: Recombinant

Proteineigenschaft: AA 1-1082

Spezies: Maus

Quelle: Tobacco (Nicotiana tabacum)

Aufreinigungstag / Konjugat: Dieses REST Protein ist gelabelt mit Strep Tag.

Applikation: Western Blotting (WB), ELISA, SDS-PAGE (SDS)

Sequenz: MATQVMGQSS GGGSLFNNSA NMGMALTNDM YDLHELSKAE LAAPQLIMLA NVALTGEASG SCCDYLVGEE RQMAELMPVG DNHFSESEGE GLEESADLKG LENMELGSLE LSAVEPQPVF EASAAPEIYS ANKDPAPETP VAEDKCRSSK AKPFRCKPCQ YEAESEEQFV HHIRIHSAKK FFVEESAEKQ AKAWESGSSP AEEGEFSKGP IRCDRCGYNT NRYDHYMAHL KHHLRAGENE RIYKCIICTY TTVSEYHWRK HLRNHFPRKV YTCSKCNYFS DRKNNYVQHV RTHTGERPYK CELCPYSSSQ KTHLTRHMRT HSGEKPFKCD QCNYVASNQH EVTRHARQVH NGPKPLNCPH CDYKTADRSN FKKHVELHVN PRQFNCPVCD YAASKKCNLQ YHFKSKHPTC PSKTMDVSKV KLKKTKKREA DLLNNAVSNE KMENEQTKTK GDVSGKKNEK PVKAVGKDAS KEKKPGSSVS VVQVTTRTRK SAVAAETKAA EVKHTDGQTG NNPEKPCKAK KNKRKKDAEA HPSEEPVNEG PVTKKKKKSE CKSKIGTNVP KGGGRAEERP GVKKQSASLK KGTKKTPPKT KTSKKGGKLA PKGMGQTEPS SGALAQVGVS PDPALIQAEV TGSGSSQTEL PSPMDIAKSE PAQMEVSLTG PPPVEPAQME PSPAKPPQVE APTYPQPPQR GPAPPTGPAP PTGPAPPTEP APPTGLAEME PSPTEPSQKE PPPSMEPPCP EELPQAEPPP MEDCQKELPS PVEPAQIEVA QTAPTQVQEE PPPVSEPPRV KPTKRSSLRK DRAEKELSLL SEMARQEQVL MGVGLVPVRD SKLLKGNKSA QDPPAPPSPS PKGNSREETP KDQEMVSDGE GTIVFPLKKG GPEEAGESPA ELAALKESAR VSSSEQNSAM PEGGASHSKC QTGSSGLCDV DTEQKTDTVP MKDSAAEPVS PPTPTVDRDA GSPAVVASPP ITLAENESQE IDEDEGIHSH DGSDLSDNMS EGSDDSGLHG ARPTPPEATS KNGKAGLAGK VTEGEFVCIF CDRSFRKEKD YSKHLNRHLV NVYFLEEAAE EQEEQEEREE QE
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Produktmerkmale: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Aufreinigung: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Reinheit: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin-Niveau: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Güteklasse: Crystallography grade

Anwendungsinformationen

Applikationshinweise: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Kommentare:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C.

Haltbarkeit: Unlimited (if stored properly)

Antigendetails

Target: REST (RE1-Silencing Transcription Factor (REST))

Andere Bezeichnung: Rest (REST Produkte)

Synonyme: NRSF Protein, XBR Protein, 2610008J04Rik Protein, AA407358 Protein, xbr Protein, nrsf Protein, rest Protein, resta Protein, REST Protein, RE1 silencing transcription factor Protein, RE1-silencing transcription factor Protein, RE1-silencing transcription factor S homeolog Protein, REST Protein, Rest Protein, rest.S Protein, rest Protein, LOC615792 Protein

Hintergrund: RE1-silencing transcription factor (Neural-restrictive silencer factor),FUNCTION: Transcriptional repressor which binds neuron-restrictive silencer element (NRSE) and represses neuronal gene transcription in non-neuronal cells (PubMed:29961578, PubMed:9771705). Restricts the expression of neuronal genes by associating with two distinct corepressors, SIN3A and RCOR1, which in turn recruit histone deacetylase to the promoters of REST-regulated genes (By similarity). Mediates repression by recruiting the BHC complex at RE1/NRSE sites which acts by deacetylating and demethylating specific sites on histones, thereby acting as a chromatin modifier (By similarity). Transcriptional repression by REST-CDYL via the recruitment of histone methyltransferase EHMT2 may be important in transformation suppression (By similarity). Represses the expression of SRRM4 in non-neural cells to prevent the activation of neural-specific splicing events and to prevent production of REST isoform 2 (PubMed:21884984). Repressor activity may be inhibited by forming heterodimers with isoform 2, thereby preventing binding to NRSE or binding to corepressors and leading to derepression of target genes (PubMed:10490617, PubMed:11039732). Also maintains repression of neuronal genes in neural stem cells, and allows transcription and differentiation into neurons by dissociation from RE1/NRSE sites of target genes (PubMed:15907476). Thereby is involved in maintaining the quiescent state of adult neural stem cells and preventing premature differentiation into mature neurons (PubMed:27819263). Plays a role in the developmental switch in synaptic NMDA receptor composition during postnatal development, by repressing GRIN2B expression and thereby altering NMDA receptor properties from containing primarily GRIN2B to primarily GRIN2A subunits (By similarity). Acts as a regulator of osteoblast differentiation (PubMed:25727884). Key repressor of gene expression in hypoxia, represses genes in hypoxia by direct binding to an RE1/NRSE site on their promoter regions (By similarity). May also function in stress resistance in the brain during aging, possibly by regulating expression of genes involved in cell death and in the stress response (PubMed:24670762). Repressor of gene expression in the hippocampus after ischemia by directly binding to RE1/NRSE sites and recruiting SIN3A and RCOR1 to promoters of target genes, thereby promoting changes in chromatin modifications and ischemia-induced cell death (By similarity). After ischemia, might play a role in repression of miR-132 expression in hippocampal neurons, thereby leading to neuronal cell death (By similarity). {ECO:0000250|UniProtKB:O54963, ECO:0000250|UniProtKB:Q13127, ECO:0000269|PubMed:10490617, ECO:0000269|PubMed:11039732, ECO:0000269|PubMed:15907476, ECO:0000269|PubMed:21884984, ECO:0000269|PubMed:24670762, ECO:0000269|PubMed:25727884, ECO:0000269|PubMed:27819263, ECO:0000269|PubMed:29961578, ECO:0000269|PubMed:9771705}., FUNCTION: [Isoform 2]: Binds to the 3' region of the neuron-restrictive silencer element (NRSE), with lower affinity than isoform 1 (PubMed:11039732). Exhibits weaker repressor activity compared to isoform 1 (By similarity). May negatively regulate the repressor activity of isoform 1 by binding to isoform 1, thereby preventing its binding to NRSE and leading to derepression of target genes (PubMed:11039732, PubMed:10490617). However, in another study, does not appear to be implicated in repressor activity of a NRSE motif-containing reporter construct nor in inhibitory activity on the isoform 1 transcriptional repressor activity (By similarity). Post-transcriptional inactivation of REST by SRRM4-dependent alternative splicing into isoform 2 is required in mechanosensory hair cells in the inner ear for derepression of neuronal genes, maintenance of hair cells and hearing (PubMed:29961578). {ECO:0000250|UniProtKB:Q13127, ECO:0000269|PubMed:10490617, ECO:0000269|PubMed:11039732, ECO:0000269|PubMed:29961578}.

Molekulargewicht: 117.8 kDa

UniProt: Q8VIG1

Pathways: Negative Regulation of Hormone Secretion, Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Chromatin Binding, Positive Regulation of Endopeptidase Activity