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ARAP3 Protein (AA 1-1538) (His tag)

Crystallography grade ARAP3 Spezies: Maus Wirt: Insektenzellen Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, Crys, SDS
Produktnummer ABIN3136685
  • Target Alle ARAP3 Proteine anzeigen
    ARAP3 (ArfGAP with RhoGAP Domain, Ankyrin Repeat and PH Domain 3 (ARAP3))
    Protein-Typ
    Recombinant
    Proteineigenschaft
    AA 1-1538
    Spezies
    Maus
    Quelle
    • 1
    • 1
    • 1
    Insektenzellen
    Aufreinigungstag / Konjugat
    Dieses ARAP3 Protein ist gelabelt mit His tag.
    Applikation
    ELISA, Western Blotting (WB), Crystallization (Crys), SDS-PAGE (SDS)
    Sequenz
    MAAPQDLDIA VWLALVHLEQ YADTFRRHGL ATAGAAQHLG HEELRHLGIS ATGHRKRILR LLRAGSAEGF LDSHLDNTME PTPSPAPDAQ PPKPVPKPRT VFGLSNPATA QRPGLSPIFW DPEVSRNSEC TQRSSPLLPS SSEQPSVPNT MEMMPNAIYF GLDLRGRAQA AQDVTPDSSQ ATVPTPAFRP TTGTVHIMDP GCLYYGVQPV GIPGASDRRD GRGVCQERAE HRQDLETRED AGYASLELPG DSILSLPTQD AETSDDLISP YASFSSTADR PVPLLSGWLD KLSPQGNYVF QRRFVQFNGR SLMYFGSDKD PFPKGVIPLT AIEMTRSSKD NKFQVITGQR VFVFRTESEA QRDLWCSTLQ SCLKEQRLLG HPRPPHPPRP LRTGTLELRG HKAKVFAALI PGELALYKSE QAFSLGIGIC FIELQGCSVR ETKSRSFDLL TPHRCFSFTA ESGGARQSWA AALQEAVTET LSDYEVAEKV WSNPANRHCA DCRASRPDWA AVNLGVVICK QCAGQHRALG SGISKVQSLK LDTSVWSNEI VQLFIVLGND RANCFWAGAL PPGEGLHPDS APGPRGEFIS RKYKLGLFRK PHPRHPDHSQ LLQALCAAMA GPNLLKNMAQ LLCVETSEGE EPLSPSALNG SLLSLLPSDS PGVYNEVVVP ATYRGFLYCG SISNKAGAPP LRRGRDAPPR LWCVLGAALE MFASESSPEP LSLLQPQDIV CLGVSPPPAD PGDLDRFPFS FELILTGGRI QHFATDGADS LEAWISAVGK WFSPLSCHQL LGPGLLRMGR LWLRSPSHAG LAPGLWLSGF GLLRGDHLFL CPAPGPGPPA PEDMVHLRRL QEISVVSAAD TPDKKEHLVL VETGRTLYLQ GEGRLDFAAW NTAIGGAAGG GGTGLQEQQM SRGDIPIIVD ACISFVTQHG LRLEGVYRKG GARARSLRLL AEFRRDARSV KLRPREHFVE DVTDTLKRFF RELDDPVTSA RLLPRWREAA ELSQKNQRLE KYKEVISCLP RVNRRTLATL IGHLYRVQKC ASLNQMCTRN LALLFAPSVF QTDGRGEHEV RVLQELIDGY ISVFDIDSDQ AAQIDLEVSL ITTWKDVQLS QAGDLIMEVY IEQQLPDNCV TLKVSPTLTA EELTNQVLEM RGAASGTDLW VTFEILEHGE LERPLHPKEK VLEQALQWCQ LPEPCSASLL LRKVSMAHAG CLFTGVRRES PRVGLLRCRE EPPRLLGNRF QERFFLVRGR CLLLLKEKKS SKPEREWSLE GAKVYLGIRK KLKPPTLWGF TLILEKMHLC LSCMDEEEMW DWTTSILKAQ HDDQQSVVLR RRSSSDLARQ KFGTMPLLPI RGDDSGATLL SANQTLRRLH NRRTLSMFFP MKSPQGSVEE QDELEEPVYE EPVYEEVGAF PELTKDTTFS STWEWSAKSD PSLTSQRSFD QPPLSKASML GHEERIPDPP PGPPSKSSSQ ARGSLEEQLL QELNNLILRK GEPASCPESS SQPTSPQAPS PTSLPTPTPS LPTQPPCTSN PPSSQPLT
    Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
    Produktmerkmale
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Mouse Arap3 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    Aufreinigung
    Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
    1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
    2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    Reinheit
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    Sterilität
    0.22 μm filtered
    Endotoxin-Niveau
    Protein is endotoxin free.
    Güteklasse
    Crystallography grade
    Top Product
    Discover our top product ARAP3 Protein
  • Applikationshinweise
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
    Kommentare

    Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Buffer
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    Handhabung
    Avoid repeated freeze-thaw cycles.
    Lagerung
    -80 °C
    Informationen zur Lagerung
    Store at -80°C.
    Haltbarkeit
    Unlimited (if stored properly)
  • Target
    ARAP3 (ArfGAP with RhoGAP Domain, Ankyrin Repeat and PH Domain 3 (ARAP3))
    Andere Bezeichnung
    Arap3 (ARAP3 Produkte)
    Synonyme
    CENTD3 Protein, ARAP3 Protein, Arap3-ps1 Protein, Centd3 Protein, RGD1304938 Protein, DRAG1 Protein, Drag1 Protein, E030006K04Rik Protein, cnt-d3 Protein, ArfGAP with RhoGAP domain, ankyrin repeat and PH domain 3 Protein, ARAP3 Protein, arap3 Protein, Arap3 Protein
    Hintergrund
    Phosphatidylinositol 3,4,5-trisphosphate-dependent GTPase-activating protein that modulates actin cytoskeleton remodeling by regulating ARF and RHO family members. Is activated by phosphatidylinositol 3,4,5-trisphosphate (PtdIns(3,4,5)P3) binding. Can be activated by phosphatidylinositol 3,4-bisphosphate (PtdIns(3,4,5)P2) binding, albeit with lower efficiency. Acts preferentially on ARF5 and on RHOA. {ECO:0000269|PubMed:15546919}.
    Molekulargewicht
    170.7 kDa Including tag.
    UniProt
    Q8R5G7
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