DCLRE1C Protein (AA 1-705) (Strep Tag)

Produktnummer ABIN3136608

Produktdetails

Target: DCLRE1C (DNA Cross-Link Repair 1C (DCLRE1C))

Protein-Typ: Recombinant

Proteineigenschaft: AA 1-705

Spezies: Maus

Quelle: Tobacco (Nicotiana tabacum)

Aufreinigungstag / Konjugat: Dieses DCLRE1C Protein ist gelabelt mit Strep Tag.

Applikation: ELISA, Western Blotting (WB), SDS-PAGE (SDS)

Sequenz: MSSFQGQMAE YPTISIDRFD RENLKARAYF LSHCHKDHMK GLRAPSLKRR LECSLKVFLY CSPVTKELLL TSPKYRFWEN RIITIEIETP TQISLVDEAS GEKEEVVVTL LPAGHCPGSV MFLFQGSNGT VLYTGDFRLA KGEASRMELL HSGGRVKDIQ SVYLDTTFCD PRFYQIPSRE QCLRGILELV RSWVTRSPHH VVWLNCKAAY GYEYLFTNLS EELGVQVHVD KLDMFKNMPD ILHHLTTDRN TQIHACRHPK AEECFQWNKL PCGITSQNKT ALHTISIKPS TMWFGERTRK TNVIVRTGES SYRACFSFHS SFSEIKDFLS YICPVNVYPN VIPVGLTVDK VMDVLKPLCR SPQSVEPKYK PLGKLKRART IHLDSEEDDD LFDDPLPTPL RHKVPYQLTL QPELFSMKAL PLDQPELRQS PGGCKAESVW SPSLANFIDC EESNSDSGEE LETPPPSLQG GLGPSTLVQQ NADPDVDIPQ WEVFFKRRDE ITGECLEHLP SSIETGGSQS PKLCSDSPKL CSDSPKLCSD SDGDSTHISS QNSSQSTHIT DQGSQGWDSQ CDTVLLSSQE KSGGDSTSLN KGAYKPKLKE SISASQIEQD ALCPQDTHCD LKSRAEVNGA PCLVELDTLS GRKSPPEKTL LSSTRADSQS SSDFEIPSTP EAELPTPEHL QCLYRKLATG QSIVVEKRKC SLLDS
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Produktmerkmale: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Aufreinigung: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Reinheit: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin-Niveau: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Güteklasse: Crystallography grade

Anwendungsinformationen

Applikationshinweise: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Kommentare:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C.

Haltbarkeit: Unlimited (if stored properly)

Antigendetails

Target: DCLRE1C (DNA Cross-Link Repair 1C (DCLRE1C))

Andere Bezeichnung: Dclre1c (DCLRE1C Produkte)

Synonyme: artemis Protein, A-SCID Protein, DCLREC1C Protein, RS-SCID Protein, SCIDA Protein, SNM1C Protein, hSNM1C Protein, Snm1l Protein, nuclease Protein, 9930121L06Rik Protein, AI661365 Protein, Art Protein, DNA cross-link repair 1C Protein, DCLRE1C Protein, Dclre1c Protein

Hintergrund: Protein artemis (mArt) (EC 3.1.-.-) (DNA cross-link repair 1C protein) (SNM1-like protein),FUNCTION: Required for V(D)J recombination, the process by which exons encoding the antigen-binding domains of immunoglobulins and T-cell receptor proteins are assembled from individual V, (D), and J gene segments. V(D)J recombination is initiated by the lymphoid specific RAG endonuclease complex, which generates site specific DNA double strand breaks (DSBs). These DSBs present two types of DNA end structures: hairpin sealed coding ends and phosphorylated blunt signal ends. These ends are independently repaired by the non homologous end joining (NHEJ) pathway to form coding and signal joints respectively. This protein likely exhibits single-strand specific 5'-3' exonuclease activity in isolation, and may acquire endonucleolytic activity on 5' and 3' hairpins and overhangs when in a complex with PRKDC. The latter activity may be required specifically for the resolution of closed hairpins prior to the formation of the coding joint. May also be required for the repair of complex DSBs induced by ionizing radiation, which require substantial end-processing prior to religation by NHEJ. {ECO:0000269|PubMed:12504013, ECO:0000269|PubMed:12615897, ECO:0000269|PubMed:15699179}.

Molekulargewicht: 78.8 kDa

UniProt: Q8K4J0

Pathways: DNA Reparatur