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OPTN Protein (AA 1-584) (His tag)

Crystallography grade OPTN Spezies: Maus Wirt: Insektenzellen Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Produktnummer ABIN3136583
  • Target Alle OPTN Proteine anzeigen
    OPTN (Optineurin (OPTN))
    Protein-Typ
    Recombinant
    Proteineigenschaft
    AA 1-584
    Spezies
    • 6
    • 1
    • 1
    Maus
    Quelle
    • 3
    • 2
    • 1
    • 1
    • 1
    Insektenzellen
    Aufreinigungstag / Konjugat
    Dieses OPTN Protein ist gelabelt mit His tag.
    Applikation
    Western Blotting (WB), SDS-PAGE (SDS), ELISA, Crystallization (Crys)
    Sequenz
    MSHQPLSCLT EKGDSPCETP GNGPSNMVHP SLDTFTPEEL LQQMKELLVE NHQLKEAMKL NNQAMKGRFE ELSAWTEKQK EERLLFEMQS KEVKERLKAL THENERLKEE LGKFKEKSEK PLEDLTGGYR YPRALEEEVE KLKTQVEQEV EHLKIQVMRL RAEKADLLGI VSELQLKLNS GGSSEDSFVE IRMTEGETEG AMKEMKNCPT PTRTDPISLS NCTEDARSCA EFEELTVSQL LLCLREGNQK VERLEVALRE AKERISDFEK KANGHSSTEK QTARRADREK EDKGQESVGS EVETLSIQVT SLFKELQEAH TKLSEAELMK KRLQEKCQAL ERKNSATPSE LNEKQELVYS NKKLELQVES MRSEIKMEQA KTEEEKSRLA TLQATHNKLL QEHNKALKTI EELTKQQAEK VDKMLLQELS EKLELAEQAL ASKQLQMDEM KQTLAKQEED LETMAVLRAQ MEVYCSDFHA ERAAREKIHE EKEQLALQLA ILLKENNDIE EGGSRQSLME MQCRHGARTS DSDQQTYLFQ RGAEDRSWQH GQQPRSIPIH SCPKCGEVLP DIDTLQIHVM DCII
    Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
    Produktmerkmale
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Mouse Optn Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    Aufreinigung
    Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
    1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
    2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    Reinheit
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    Sterilität
    0.22 μm filtered
    Endotoxin-Niveau
    Protein is endotoxin free.
    Güteklasse
    Crystallography grade
    Top Product
    Discover our top product OPTN Protein
  • Applikationshinweise
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
    Kommentare

    Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Buffer
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    Handhabung
    Avoid repeated freeze-thaw cycles.
    Lagerung
    -80 °C
    Informationen zur Lagerung
    Store at -80°C.
    Haltbarkeit
    Unlimited (if stored properly)
  • Target
    OPTN (Optineurin (OPTN))
    Andere Bezeichnung
    Optn (OPTN Produkte)
    Synonyme
    nrp Protein, fip2 Protein, hip7 Protein, hypl Protein, glc1e Protein, tfiiia-intp Protein, ALS12 Protein, FIP2 Protein, GLC1E Protein, HIP7 Protein, HYPL Protein, NRP Protein, TFIIIA-INTP Protein, Fip2 Protein, 4930441O07Rik Protein, fj52f04 Protein, si:ch211-240l19.3 Protein, wu:fj52f04 Protein, zgc:66386 Protein, zgc:77868 Protein, optineurin Protein, optineurin L homeolog Protein, OPTN Protein, optn Protein, Optn Protein, optn.L Protein
    Hintergrund
    Plays an important role in the maintenance of the Golgi complex, in membrane trafficking, in exocytosis, through its interaction with myosin VI and Rab8. Links myosin VI to the Golgi complex and plays an important role in Golgi ribbon formation. Negatively regulates the induction of IFNB in response to RNA virus infection. Plays a neuroprotective role in the eye and optic nerve. Probably part of the TNF-alpha signaling pathway that can shift the equilibrium toward induction of cell death. May act by regulating membrane trafficking and cellular morphogenesis via a complex that contains Rab8 and hungtingtin (HD). Mediates the interaction of Rab8 with the probable GTPase-activating protein TBC1D17 during Rab8-mediated endocytic trafficking, such as of transferrin receptor (TFRC/TfR), regulates Rab8 recruitnment to tubules emanating from the endocytic recycling compartment. Autophagy receptor that interacts directly with both the cargo to become degraded and an autophagy modifier of the MAP1 LC3 family, targets ubiquitin-coated bacteria (xenophagy) and appears to function in the same pathway as SQSTM1 and CALCOCO2/NDP52 (By similarity). {ECO:0000250}.
    Molekulargewicht
    68.0 kDa Including tag.
    UniProt
    Q8K3K8
    Pathways
    M Phase
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