INPP5B Protein (AA 1-990) (His tag)

Produktnummer ABIN3136571

Produktdetails

Target: INPP5B (Inositol Polyphosphate-5-Phosphatase, 75kDa (INPP5B))

Protein-Typ: Recombinant

Proteineigenschaft: AA 1-990

Spezies: Maus

Quelle: Insektenzellen

Aufreinigungstag / Konjugat: Dieses INPP5B Protein ist gelabelt mit His tag.

Applikation: ELISA, Western Blotting (WB), Crystallization (Crys), SDS-PAGE (SDS)

Sequenz: MDQSVAIQET LVEGEYCVIA VQGVLCKGDS RQSRLLGLVR YRLENDAQEH ALFLYTHRRM AITGDDVSLD QIVPLSKDFM LEEVSPDGEL YILGSDVTVQ LNTAELKLVF QLPFGSHTRT FLQEVARACP GFDPETRDPE FEWLSRHTCA EPDAESPKPR EWNSDPGTRS GFAPIGGSRH QSRNARRGLE DVLPRGPGYI LLWGGAAEEP EFLLAEEMHE GGPVRGRRPL AGRRDEALEE ADWEMSAGGG SRERDCAGVS NVDSSRPNGR GPDQPSGARC PEKPENSLTR QNKSKSDMSE KVRSATVTVS DKAHILSVQK FGLRDTIVRS HLVQKEENYT YIQNFRFFVG TYNVNGQSPK ECLRPWLSHS ALAPDVYCVG FQELDLSKEA FFFHDTPKEE EWFKAVSESL HPDAKYAKVK FVRLVGIMLL LYVKQEHAAY ISEVEAETVG TGIMGRMGNK GGVAIRFQLH NTSICVVNSH LAAHTEEYER RNQDYRDICS RMQFPQVDPS QPPLTINKHD VILWLGDLNY RIEELDVGKV KKLVEEKAFQ TLYAHDQLKI QVAARTIFDG FTEGEITFQP TYKYDTGSDD WDTSEKCRAP AWCDRILWKG KNITQLSYQS HMALKTSDHK PVSSVFDIGV RVVNEELYRK TLEEIVRSLD KMENANIPSV TLSKREFCFE NVKYMQLQTE SFTIHNSQVP CQFEFINKPD EESYCKQWLT ARPSKGFLLP DSHVEIELEL FVNKSTATKL NSGKDTIEDI LVLHLERGKD YFLSVSGNYL PSCFGSPIHT LCYMREPILD LPLKTVSDLT LMSVQTADDR SQLENPMEIP KELWMMVDYL YRNAVQQEDL FQQPGLRPEF DHIRDCLDTG MIDQLCANNH SVAEALLLFL ESLPEPVICY SAYHSCLECS GNYAASKQII LTLPSFHKNV FNYLMAFLQE LLKNSANNHL DENILASIFG SLLLRNPARH QKLDMAEKKK AQEFIHQFLC
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Produktmerkmale:

• Made in Germany - from design to production - by highly experienced protein experts.
• Mouse Inpp5b Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Aufreinigung: Two step purification of proteins expressed in baculovirus infected SF9 insect cells:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Reinheit: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Sterilität: 0.22 μm filtered

Endotoxin-Niveau: Protein is endotoxin free.

Güteklasse: Crystallography grade

Anwendungsinformationen

Applikationshinweise: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Kommentare:

Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C.

Haltbarkeit: Unlimited (if stored properly)

Antigendetails

Target: INPP5B (Inositol Polyphosphate-5-Phosphatase, 75kDa (INPP5B))

Andere Bezeichnung: Inpp5b (INPP5B Produkte)

Synonyme: 75kDa Protein, AW260155 Protein, INPP5P Protein, 5PTase Protein, inositol polyphosphate-5-phosphatase B Protein, type II inositol 1,4,5-trisphosphate 5-phosphatase-like Protein, inositol polyphosphate-5-phosphatase B L homeolog Protein, inositol polyphosphate 5-phosphatase OCRL-1 Protein, INPP5B Protein, LOC100224437 Protein, inpp5b.L Protein, LOC100159919 Protein, Inpp5b Protein

Hintergrund: Hydrolyzes phosphatidylinositol 4,5-bisphosphate (PtIns(4,5)P2) and the signaling molecule phosphatidylinositol 1,4,5-trisphosphate (PtIns(1,4,5)P3), and thereby modulates cellular signaling events. {ECO:0000269|PubMed:11311145, ECO:0000269|PubMed:9525932}.

Molekulargewicht: 113.4 kDa Including tag.

UniProt: Q8K337