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PAF1/PD2 Protein (AA 1-535) (His tag)

Crystallography grade PAF1 Spezies: Maus Wirt: Escherichia coli (E. coli) Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, Crys, SDS, WB
Produktnummer ABIN3136561
  • Target Alle PAF1/PD2 (PAF1) Proteine anzeigen
    PAF1/PD2 (PAF1) (Paf1, RNA Polymerase II Associated Factor, Homolog (PAF1))
    Protein-Typ
    Recombinant
    Proteineigenschaft
    AA 1-535
    Spezies
    • 1
    • 1
    Maus
    Quelle
    • 1
    • 1
    Escherichia coli (E. coli)
    Aufreinigungstag / Konjugat
    Dieses PAF1/PD2 Protein ist gelabelt mit His tag.
    Applikation
    ELISA, Crystallization (Crys), SDS-PAGE (SDS), Western Blotting (WB)
    Sequenz
    MAPTIQTQAQ REDGHRPNSH RTLPERSGVV CRVKYCNSLP DIPFDPKFIT YPFDQNRFVQ YKATSLEKQH KHDLLTEPDL GVTIDLINPD TYRIDPNVLL DPADEKLLEE EIQAPTSSKR SQQHAKVVPW MRKTEYISTE FNRYGISNEK PEVKIGVSVK QQFTEEEIYK DRDSQITAIE KTFEDAQKSI SQHYSKPRVT PVEVMPVFPD FKMWINPCAQ VIFDSDPAPK DTSGAAALEM MSQAMIRGMM DEEGNQFVAY FLPVEETLKK RKRDQEEEMD YAPDDVYDYK IAREYNWNVK NKASKGYEEN YFFIFREGDG VYYNELETRV RLSKRRAKAG VQSGTNALLV VKHRDMNEKE LEAQEARKAQ LENHEPEEEE EEEMEAEEKE AGGSDEEQEK GSSSEKEGSE DEHSGSESDR EEGDRDEASD KSGSGEDESS EDEARAARDK EEIFGSDADS EDDADSDDED RGQAHRGSDN DSDSGSDGGG QRSRSQSRSR SRSASPFPSG SEHSAQEDGS EAAASDSSEA DSDSD
    Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
    Produktmerkmale
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Mouse Paf1 Protein (raised in E. Coli) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    Aufreinigung
    Two step purification of proteins expressed in bacterial culture:
    1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
    2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    Reinheit
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    Sterilität
    0.22 μm filtered
    Endotoxin-Niveau
    Endotoxin has not been removed. Please contact us if you require endotoxin removal.
    Güteklasse
    Crystallography grade
    Top Product
    Discover our top product PAF1 Protein
  • Applikationshinweise
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
    Kommentare

    Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Buffer
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    Handhabung
    Avoid repeated freeze-thaw cycles.
    Lagerung
    -80 °C
    Informationen zur Lagerung
    Store at -80°C.
    Haltbarkeit
    Unlimited (if stored properly)
  • Target
    PAF1/PD2 (PAF1) (Paf1, RNA Polymerase II Associated Factor, Homolog (PAF1))
    Andere Bezeichnung
    Paf1 (PAF1 Produkte)
    Synonyme
    F23149_1 Protein, PD2 Protein, 5730511K23Rik Protein, AA675045 Protein, f23149_1 Protein, pd2 Protein, im:6906070 Protein, paf1l Protein, wu:fb80a09 Protein, paf1 Protein, RGD1306219 Protein, PAF1 homolog, Paf1/RNA polymerase II complex component Protein, Paf1, RNA polymerase II complex component Protein, PAF1 homolog, Paf1/RNA polymerase II complex component L homeolog Protein, RNA polymerase II-associated factor 1 homolog pseudogene Protein, RNA polymerase II-associated factor 1 homolog Protein, paf1, RNA polymerase II associated factor, homolog (S. cerevisiae) Protein, Paf1, RNA polymerase II associated factor, homolog (S. cerevisiae) Protein, PAF1 Protein, Paf1 Protein, paf1 Protein, paf1.L Protein, LOC100019208 Protein, LOC100161171 Protein, LOC100639019 Protein, LOC100650306 Protein
    Hintergrund
    Component of the PAF1 complex (PAF1C) which has multiple functions during transcription by RNA polymerase II and is implicated in regulation of development and maintenance of embryonic stem cell pluripotency. PAF1C associates with RNA polymerase II through interaction with POLR2A CTD non-phosphorylated and 'Ser-2'- and 'Ser-5'-phosphorylated forms and is involved in transcriptional elongation, acting both indepentently and synergistically with TCEA1 and in cooperation with the DSIF complex and HTATSF1. PAF1C is required for transcription of Hox and Wnt target genes. PAF1C is involved in hematopoiesis and stimulates transcriptional activity of KMT2A/MLL1. PAF1C is involved in histone modifications such as ubiquitination of histone H2B and methylation on histone H3 'Lys-4' (H3K4me3). PAF1C recruits the RNF20/40 E3 ubiquitin-protein ligase complex and the E2 enzyme UBE2A or UBE2B to chromatin which mediate monoubiquitination of 'Lys-120' of histone H2B (H2BK120ub1), UB2A/B-mediated H2B ubiquitination is proposed to be coupled to transcription. PAF1C is involved in mRNA 3' end formation probably through association with cleavage and poly(A) factors. Connects PAF1C with the RNF20/40 E3 ubiquitin-protein ligase complex. Involved in polyadenylation of mRNA precursors (By similarity). {ECO:0000250, ECO:0000269|PubMed:19345177}.
    Molekulargewicht
    61.5 kDa Including tag.
    UniProt
    Q8K2T8
    Pathways
    Cellular Response to Molecule of Bacterial Origin, Stem Cell Maintenance
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